Impaired expression of innate immunity-related genes in IgG4-related disease: A possible mechanism in the pathogenesis of IgG4-RD

Takuji Nakamura; Tomomi Satoh-Nakamura; Akio Nakajima; Takafumi Kawanami; Tomoyuki Sakai; Yoshimasa Fujita; Haruka Iwao; Miyuki Miki; Yasufumi Masaki; Toshiro Okazaki; Yasuhito Ishigaki; Mitsuhiro Kawano; Kazunori Yamada; Shoko Matsui; Takako Saeki; Terumi Kamisawa; Motohisa Yamamoto; Hideaki Hamano; Tomoki Origuchi; Shintaro Hirata; Yoshiya Tanaka; Hiroto Tsuboi; Takayuki Sumida; Kazuichi Okazaki; Masao Tanaka; Tsutomu Chiba; Tsuneyo Mimori; Hisanori Umehara

doi:10.1080/14397595.2019.1621475

Impaired expression of innate immunity-related genes in IgG4-related disease: A possible mechanism in the pathogenesis of IgG4-RD

Takuji Nakamura^*, Tomomi Satoh-Nakamura, Akio Nakajima, Takafumi Kawanami, Tomoyuki Sakai, Yoshimasa Fujita, Haruka Iwao, Miyuki Miki, Yasufumi Masaki, Toshiro Okazaki, Yasuhito Ishigaki, Mitsuhiro Kawano, Kazunori Yamada, Shoko Matsui, Takako Saeki, Terumi Kamisawa, Motohisa Yamamoto, Hideaki Hamano, Tomoki Origuchi, Shintaro HirataYoshiya Tanaka, Hiroto Tsuboi, Takayuki Sumida, Kazuichi Okazaki, Masao Tanaka, Tsutomu Chiba, Tsuneyo Mimori, Hisanori Umehara

^*Corresponding author for this work

Center for Health Care and Human Sciences

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Background: IgG4-related disease (IgG4-RD) is characterized by elevated serum IgG4 and tissue infiltration by IgG4-positive plasma cells. The pathogenesis of this disease is not clear. Transcriptome analysis was performed to identify genes over- and under-expressed in patients with IgG4-RD. Method: DNA microarray analysis was performed using RNA from peripheral blood mononuclear cells of two patients with IgG4-RD and four healthy individuals. Genes showing a greater than threefold change in expression in IgG4-RD patients following steroid therapy were identified. Four genes related to innate immunity such as transcobalamin I (TCN1), secretory leukocyte peptidase inhibitor (SLPI), bactericidal/permeability-increasing protein (BPI) and lactotransferrin (LTF) were assessed by real-time PCR in 15 IgG4-RD patients and 13 healthy individuals. Result: DNA microarray analysis identified 30 genes showing a greater than threefold change in expression in IgG4-RD patients following steroid therapy. Real-time RT-PCR showed that the levels of mRNAs encoding TCNI and SLPI, except for BPI and LTF, were significantly lower in patients with IgG4-RD than in healthy people. The levels of all four mRNAs in patients with IgG4-RD were significantly increased after steroid treatment. Conclusion: These results indicate that reduction in expression of innate immunity-related genes may participate in the pathogenesis of IgG4-RD that steroid treatment may rectify impaired innate immunity as well as acquired immunity.

Original language	English
Pages (from-to)	551-557
Number of pages	7
Journal	Modern Rheumatology
Volume	30
Issue number	3
DOIs	https://doi.org/10.1080/14397595.2019.1621475
State	Published - 2020/05/03

Keywords

DNA microarray analysis
IgG4-related disease
acquired immunity
innate immunity
innate immunity related gene

ASJC Scopus subject areas

General Medicine

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10.1080/14397595.2019.1621475

Cite this

Nakamura, T., Satoh-Nakamura, T., Nakajima, A., Kawanami, T., Sakai, T., Fujita, Y., Iwao, H., Miki, M., Masaki, Y., Okazaki, T., Ishigaki, Y., Kawano, M., Yamada, K., Matsui, S., Saeki, T., Kamisawa, T., Yamamoto, M., Hamano, H., Origuchi, T., ... Umehara, H. (2020). Impaired expression of innate immunity-related genes in IgG4-related disease: A possible mechanism in the pathogenesis of IgG4-RD. Modern Rheumatology, 30(3), 551-557. https://doi.org/10.1080/14397595.2019.1621475

@article{4bf8e29c9d0f4b15acf6b49604ce6825,

title = "Impaired expression of innate immunity-related genes in IgG4-related disease: A possible mechanism in the pathogenesis of IgG4-RD",

abstract = "Background: IgG4-related disease (IgG4-RD) is characterized by elevated serum IgG4 and tissue infiltration by IgG4-positive plasma cells. The pathogenesis of this disease is not clear. Transcriptome analysis was performed to identify genes over- and under-expressed in patients with IgG4-RD. Method: DNA microarray analysis was performed using RNA from peripheral blood mononuclear cells of two patients with IgG4-RD and four healthy individuals. Genes showing a greater than threefold change in expression in IgG4-RD patients following steroid therapy were identified. Four genes related to innate immunity such as transcobalamin I (TCN1), secretory leukocyte peptidase inhibitor (SLPI), bactericidal/permeability-increasing protein (BPI) and lactotransferrin (LTF) were assessed by real-time PCR in 15 IgG4-RD patients and 13 healthy individuals. Result: DNA microarray analysis identified 30 genes showing a greater than threefold change in expression in IgG4-RD patients following steroid therapy. Real-time RT-PCR showed that the levels of mRNAs encoding TCNI and SLPI, except for BPI and LTF, were significantly lower in patients with IgG4-RD than in healthy people. The levels of all four mRNAs in patients with IgG4-RD were significantly increased after steroid treatment. Conclusion: These results indicate that reduction in expression of innate immunity-related genes may participate in the pathogenesis of IgG4-RD that steroid treatment may rectify impaired innate immunity as well as acquired immunity.",

keywords = "DNA microarray analysis, IgG4-related disease, acquired immunity, innate immunity, innate immunity related gene",

author = "Takuji Nakamura and Tomomi Satoh-Nakamura and Akio Nakajima and Takafumi Kawanami and Tomoyuki Sakai and Yoshimasa Fujita and Haruka Iwao and Miyuki Miki and Yasufumi Masaki and Toshiro Okazaki and Yasuhito Ishigaki and Mitsuhiro Kawano and Kazunori Yamada and Shoko Matsui and Takako Saeki and Terumi Kamisawa and Motohisa Yamamoto and Hideaki Hamano and Tomoki Origuchi and Shintaro Hirata and Yoshiya Tanaka and Hiroto Tsuboi and Takayuki Sumida and Kazuichi Okazaki and Masao Tanaka and Tsutomu Chiba and Tsuneyo Mimori and Hisanori Umehara",

note = "Publisher Copyright: {\textcopyright} 2019, {\textcopyright} 2019 Japan College of Rheumatology.",

year = "2020",

month = may,

day = "3",

doi = "10.1080/14397595.2019.1621475",

language = "英語",

volume = "30",

pages = "551--557",

journal = "Modern Rheumatology",

issn = "1439-7595",

publisher = "Oxford University Press",

number = "3",

}

Nakamura, T, Satoh-Nakamura, T, Nakajima, A, Kawanami, T, Sakai, T, Fujita, Y, Iwao, H, Miki, M, Masaki, Y, Okazaki, T, Ishigaki, Y, Kawano, M, Yamada, K, Matsui, S, Saeki, T, Kamisawa, T, Yamamoto, M, Hamano, H, Origuchi, T, Hirata, S, Tanaka, Y, Tsuboi, H, Sumida, T, Okazaki, K, Tanaka, M, Chiba, T, Mimori, T & Umehara, H 2020, 'Impaired expression of innate immunity-related genes in IgG4-related disease: A possible mechanism in the pathogenesis of IgG4-RD', Modern Rheumatology, vol. 30, no. 3, pp. 551-557. https://doi.org/10.1080/14397595.2019.1621475

TY - JOUR

T1 - Impaired expression of innate immunity-related genes in IgG4-related disease

T2 - A possible mechanism in the pathogenesis of IgG4-RD

AU - Nakamura, Takuji

AU - Satoh-Nakamura, Tomomi

AU - Nakajima, Akio

AU - Kawanami, Takafumi

AU - Sakai, Tomoyuki

AU - Fujita, Yoshimasa

AU - Iwao, Haruka

AU - Miki, Miyuki

AU - Masaki, Yasufumi

AU - Okazaki, Toshiro

AU - Ishigaki, Yasuhito

AU - Kawano, Mitsuhiro

AU - Yamada, Kazunori

AU - Matsui, Shoko

AU - Saeki, Takako

AU - Kamisawa, Terumi

AU - Yamamoto, Motohisa

AU - Hamano, Hideaki

AU - Origuchi, Tomoki

AU - Hirata, Shintaro

AU - Tanaka, Yoshiya

AU - Tsuboi, Hiroto

AU - Sumida, Takayuki

AU - Okazaki, Kazuichi

AU - Tanaka, Masao

AU - Chiba, Tsutomu

AU - Mimori, Tsuneyo

AU - Umehara, Hisanori

PY - 2020/5/3

Y1 - 2020/5/3

N2 - Background: IgG4-related disease (IgG4-RD) is characterized by elevated serum IgG4 and tissue infiltration by IgG4-positive plasma cells. The pathogenesis of this disease is not clear. Transcriptome analysis was performed to identify genes over- and under-expressed in patients with IgG4-RD. Method: DNA microarray analysis was performed using RNA from peripheral blood mononuclear cells of two patients with IgG4-RD and four healthy individuals. Genes showing a greater than threefold change in expression in IgG4-RD patients following steroid therapy were identified. Four genes related to innate immunity such as transcobalamin I (TCN1), secretory leukocyte peptidase inhibitor (SLPI), bactericidal/permeability-increasing protein (BPI) and lactotransferrin (LTF) were assessed by real-time PCR in 15 IgG4-RD patients and 13 healthy individuals. Result: DNA microarray analysis identified 30 genes showing a greater than threefold change in expression in IgG4-RD patients following steroid therapy. Real-time RT-PCR showed that the levels of mRNAs encoding TCNI and SLPI, except for BPI and LTF, were significantly lower in patients with IgG4-RD than in healthy people. The levels of all four mRNAs in patients with IgG4-RD were significantly increased after steroid treatment. Conclusion: These results indicate that reduction in expression of innate immunity-related genes may participate in the pathogenesis of IgG4-RD that steroid treatment may rectify impaired innate immunity as well as acquired immunity.

AB - Background: IgG4-related disease (IgG4-RD) is characterized by elevated serum IgG4 and tissue infiltration by IgG4-positive plasma cells. The pathogenesis of this disease is not clear. Transcriptome analysis was performed to identify genes over- and under-expressed in patients with IgG4-RD. Method: DNA microarray analysis was performed using RNA from peripheral blood mononuclear cells of two patients with IgG4-RD and four healthy individuals. Genes showing a greater than threefold change in expression in IgG4-RD patients following steroid therapy were identified. Four genes related to innate immunity such as transcobalamin I (TCN1), secretory leukocyte peptidase inhibitor (SLPI), bactericidal/permeability-increasing protein (BPI) and lactotransferrin (LTF) were assessed by real-time PCR in 15 IgG4-RD patients and 13 healthy individuals. Result: DNA microarray analysis identified 30 genes showing a greater than threefold change in expression in IgG4-RD patients following steroid therapy. Real-time RT-PCR showed that the levels of mRNAs encoding TCNI and SLPI, except for BPI and LTF, were significantly lower in patients with IgG4-RD than in healthy people. The levels of all four mRNAs in patients with IgG4-RD were significantly increased after steroid treatment. Conclusion: These results indicate that reduction in expression of innate immunity-related genes may participate in the pathogenesis of IgG4-RD that steroid treatment may rectify impaired innate immunity as well as acquired immunity.

KW - DNA microarray analysis

KW - IgG4-related disease

KW - acquired immunity

KW - innate immunity

KW - innate immunity related gene

UR - http://www.scopus.com/inward/record.url?scp=85068799182&partnerID=8YFLogxK

U2 - 10.1080/14397595.2019.1621475

DO - 10.1080/14397595.2019.1621475

M3 - 学術論文

C2 - 31116057

AN - SCOPUS:85068799182

SN - 1439-7595

VL - 30

SP - 551

EP - 557

JO - Modern Rheumatology

JF - Modern Rheumatology

IS - 3

ER -

Impaired expression of innate immunity-related genes in IgG4-related disease: A possible mechanism in the pathogenesis of IgG4-RD

Abstract

Keywords

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