Immuno-FET sensor

Masao Gotoh; Masayasu Suzuki; Izumi Kubo; Eiichi Tamiya; Isao Karube

doi:10.1016/0304-5102(89)80063-X

Immuno-FET sensor

Masao Gotoh^*, Masayasu Suzuki, Izumi Kubo, Eiichi Tamiya, Isao Karube

^*Corresponding author for this work

Electric and Electronic Engineering

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

An immuno-FET sensor was constructed using immobilized anti-human serum albumin, polyvinylbutyral membrane and an Ion Sensitive Field Effect Transistor (ISFET). The characteristics of this sensor system were investigated using human serum albumin (HSA) as a standard substrate. A linear relationship was obtained between the initial rate of the differential gate output voltage change and the logarithmic value of the HSA concentration. HSA was determined in the range 0.01 - 1 mg ml^-1. The effect of pH was investigated. In the alkaline range, the response increased in proportion to pH value (pH 7.0, 9.0). In contrast, in the acid range, the response was not observed (pH 3.0, 4.0). The reusability was also investigated. After five cycles, the relative activity had decreased by approximately 80%.

Original language	English
Pages (from-to)	285-292
Number of pages	8
Journal	Journal of Molecular Catalysis
Volume	53
Issue number	3
DOIs	https://doi.org/10.1016/0304-5102(89)80063-X
State	Published - 1989/08/15

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General Engineering

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title = "Immuno-FET sensor",

abstract = "An immuno-FET sensor was constructed using immobilized anti-human serum albumin, polyvinylbutyral membrane and an Ion Sensitive Field Effect Transistor (ISFET). The characteristics of this sensor system were investigated using human serum albumin (HSA) as a standard substrate. A linear relationship was obtained between the initial rate of the differential gate output voltage change and the logarithmic value of the HSA concentration. HSA was determined in the range 0.01 - 1 mg ml-1. The effect of pH was investigated. In the alkaline range, the response increased in proportion to pH value (pH 7.0, 9.0). In contrast, in the acid range, the response was not observed (pH 3.0, 4.0). The reusability was also investigated. After five cycles, the relative activity had decreased by approximately 80%.",

author = "Masao Gotoh and Masayasu Suzuki and Izumi Kubo and Eiichi Tamiya and Isao Karube",

year = "1989",

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T1 - Immuno-FET sensor

AU - Gotoh, Masao

AU - Suzuki, Masayasu

AU - Kubo, Izumi

AU - Tamiya, Eiichi

AU - Karube, Isao

PY - 1989/8/15

Y1 - 1989/8/15

N2 - An immuno-FET sensor was constructed using immobilized anti-human serum albumin, polyvinylbutyral membrane and an Ion Sensitive Field Effect Transistor (ISFET). The characteristics of this sensor system were investigated using human serum albumin (HSA) as a standard substrate. A linear relationship was obtained between the initial rate of the differential gate output voltage change and the logarithmic value of the HSA concentration. HSA was determined in the range 0.01 - 1 mg ml-1. The effect of pH was investigated. In the alkaline range, the response increased in proportion to pH value (pH 7.0, 9.0). In contrast, in the acid range, the response was not observed (pH 3.0, 4.0). The reusability was also investigated. After five cycles, the relative activity had decreased by approximately 80%.

AB - An immuno-FET sensor was constructed using immobilized anti-human serum albumin, polyvinylbutyral membrane and an Ion Sensitive Field Effect Transistor (ISFET). The characteristics of this sensor system were investigated using human serum albumin (HSA) as a standard substrate. A linear relationship was obtained between the initial rate of the differential gate output voltage change and the logarithmic value of the HSA concentration. HSA was determined in the range 0.01 - 1 mg ml-1. The effect of pH was investigated. In the alkaline range, the response increased in proportion to pH value (pH 7.0, 9.0). In contrast, in the acid range, the response was not observed (pH 3.0, 4.0). The reusability was also investigated. After five cycles, the relative activity had decreased by approximately 80%.

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VL - 53

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JO - Journal of Molecular Catalysis

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Immuno-FET sensor

Abstract

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