Identification of human ELOVL5 enhancer regions controlled by SREBP

Akito Shikama; Haruna Shinozaki; Yoshinori Takeuchi; Takashi Matsuzaka; Yuichi Aita; Tomoki Murayama; Yoshikazu Sawada; Xiaoying Piao; Naoki Toya; Yukari Oya; Ayako Takarada; Yukari Masuda; Makiko Nishi; Midori Kubota; Yoshihiko Izumida; Yoshimi Nakagawa; Hitoshi Iwasaki; Kazuto Kobayashi; Shigeru Yatoh; Hiroaki Suzuki; Hiroaki Yagyu; Yasushi Kawakami; Nobuhiro Yamada; Hitoshi Shimano; Naoya Yahagi

doi:10.1016/j.bbrc.2015.08.101

Identification of human ELOVL5 enhancer regions controlled by SREBP

Akito Shikama, Haruna Shinozaki, Yoshinori Takeuchi, Takashi Matsuzaka, Yuichi Aita, Tomoki Murayama, Yoshikazu Sawada, Xiaoying Piao, Naoki Toya, Yukari Oya, Ayako Takarada, Yukari Masuda, Makiko Nishi, Midori Kubota, Yoshihiko Izumida, Yoshimi Nakagawa, Hitoshi Iwasaki, Kazuto Kobayashi, Shigeru Yatoh, Hiroaki SuzukiHiroaki Yagyu, Yasushi Kawakami, Nobuhiro Yamada, Hitoshi Shimano, Naoya Yahagi^*

^*Corresponding author for this work

Complex Biosystem Research, Institute of Natural Medicine

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Fatty acid elongase 5 (ELOVL5) is an enzyme involved in the synthesis of polyunsaturated fatty acids. Sterol Regulatory Element-binding Protein (SREBP)-1 activates ELOVL5 and increases polyunsaturated fatty acid synthesis, which in turn negatively affects SREBP-1 expression. Thus, ELOVL5 has been established as an SREBP-1 target gene and an important component of the negative feedback loop of de novo lipogenesis. However, the human ELOVL5 promoter/enhancer has not been fully analyzed and the location of SREBP biding sites around the ELOVL5 gene has yet to be defined. Here we performed a detailed promoter/enhancer analysis of human ELOVL5 gene, and identified two new SREBP binding sites, one in the 10 kb upstream region and one in the exon 1. These two SRE motifs are conserved among mammals and the mechanism found in the present study by which SREBP activates ELOVL5 is considered to be common in mammals. Through these findings, we clarified the molecular mechanism how SREBP activates ELOVL5, an important regulator of de novo lipogenesis.

Original language	English
Pages (from-to)	857-863
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	465
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2015.08.101
State	Published - 2015/10/02

Keywords

Fatty acid synthesis
Polyunsaturated fatty acids
Transcription

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2015.08.101

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Shikama, A., Shinozaki, H., Takeuchi, Y., Matsuzaka, T., Aita, Y., Murayama, T., Sawada, Y., Piao, X., Toya, N., Oya, Y., Takarada, A., Masuda, Y., Nishi, M., Kubota, M., Izumida, Y., Nakagawa, Y., Iwasaki, H., Kobayashi, K., Yatoh, S., ... Yahagi, N. (2015). Identification of human ELOVL5 enhancer regions controlled by SREBP. Biochemical and Biophysical Research Communications, 465(4), 857-863. https://doi.org/10.1016/j.bbrc.2015.08.101

@article{3b83f318da7e4f7ea38069ab5d3ee972,

title = "Identification of human ELOVL5 enhancer regions controlled by SREBP",

abstract = "Fatty acid elongase 5 (ELOVL5) is an enzyme involved in the synthesis of polyunsaturated fatty acids. Sterol Regulatory Element-binding Protein (SREBP)-1 activates ELOVL5 and increases polyunsaturated fatty acid synthesis, which in turn negatively affects SREBP-1 expression. Thus, ELOVL5 has been established as an SREBP-1 target gene and an important component of the negative feedback loop of de novo lipogenesis. However, the human ELOVL5 promoter/enhancer has not been fully analyzed and the location of SREBP biding sites around the ELOVL5 gene has yet to be defined. Here we performed a detailed promoter/enhancer analysis of human ELOVL5 gene, and identified two new SREBP binding sites, one in the 10 kb upstream region and one in the exon 1. These two SRE motifs are conserved among mammals and the mechanism found in the present study by which SREBP activates ELOVL5 is considered to be common in mammals. Through these findings, we clarified the molecular mechanism how SREBP activates ELOVL5, an important regulator of de novo lipogenesis.",

keywords = "Fatty acid synthesis, Polyunsaturated fatty acids, Transcription",

author = "Akito Shikama and Haruna Shinozaki and Yoshinori Takeuchi and Takashi Matsuzaka and Yuichi Aita and Tomoki Murayama and Yoshikazu Sawada and Xiaoying Piao and Naoki Toya and Yukari Oya and Ayako Takarada and Yukari Masuda and Makiko Nishi and Midori Kubota and Yoshihiko Izumida and Yoshimi Nakagawa and Hitoshi Iwasaki and Kazuto Kobayashi and Shigeru Yatoh and Hiroaki Suzuki and Hiroaki Yagyu and Yasushi Kawakami and Nobuhiro Yamada and Hitoshi Shimano and Naoya Yahagi",

year = "2015",

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day = "2",

doi = "10.1016/j.bbrc.2015.08.101",

language = "英語",

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Shikama, A, Shinozaki, H, Takeuchi, Y, Matsuzaka, T, Aita, Y, Murayama, T, Sawada, Y, Piao, X, Toya, N, Oya, Y, Takarada, A, Masuda, Y, Nishi, M, Kubota, M, Izumida, Y, Nakagawa, Y, Iwasaki, H, Kobayashi, K, Yatoh, S, Suzuki, H, Yagyu, H, Kawakami, Y, Yamada, N, Shimano, H & Yahagi, N 2015, 'Identification of human ELOVL5 enhancer regions controlled by SREBP', Biochemical and Biophysical Research Communications, vol. 465, no. 4, pp. 857-863. https://doi.org/10.1016/j.bbrc.2015.08.101

TY - JOUR

T1 - Identification of human ELOVL5 enhancer regions controlled by SREBP

AU - Shikama, Akito

AU - Shinozaki, Haruna

AU - Takeuchi, Yoshinori

AU - Matsuzaka, Takashi

AU - Aita, Yuichi

AU - Murayama, Tomoki

AU - Sawada, Yoshikazu

AU - Piao, Xiaoying

AU - Toya, Naoki

AU - Oya, Yukari

AU - Takarada, Ayako

AU - Masuda, Yukari

AU - Nishi, Makiko

AU - Kubota, Midori

AU - Izumida, Yoshihiko

AU - Nakagawa, Yoshimi

AU - Iwasaki, Hitoshi

AU - Kobayashi, Kazuto

AU - Yatoh, Shigeru

AU - Suzuki, Hiroaki

AU - Yagyu, Hiroaki

AU - Kawakami, Yasushi

AU - Yamada, Nobuhiro

AU - Shimano, Hitoshi

AU - Yahagi, Naoya

PY - 2015/10/2

Y1 - 2015/10/2

N2 - Fatty acid elongase 5 (ELOVL5) is an enzyme involved in the synthesis of polyunsaturated fatty acids. Sterol Regulatory Element-binding Protein (SREBP)-1 activates ELOVL5 and increases polyunsaturated fatty acid synthesis, which in turn negatively affects SREBP-1 expression. Thus, ELOVL5 has been established as an SREBP-1 target gene and an important component of the negative feedback loop of de novo lipogenesis. However, the human ELOVL5 promoter/enhancer has not been fully analyzed and the location of SREBP biding sites around the ELOVL5 gene has yet to be defined. Here we performed a detailed promoter/enhancer analysis of human ELOVL5 gene, and identified two new SREBP binding sites, one in the 10 kb upstream region and one in the exon 1. These two SRE motifs are conserved among mammals and the mechanism found in the present study by which SREBP activates ELOVL5 is considered to be common in mammals. Through these findings, we clarified the molecular mechanism how SREBP activates ELOVL5, an important regulator of de novo lipogenesis.

AB - Fatty acid elongase 5 (ELOVL5) is an enzyme involved in the synthesis of polyunsaturated fatty acids. Sterol Regulatory Element-binding Protein (SREBP)-1 activates ELOVL5 and increases polyunsaturated fatty acid synthesis, which in turn negatively affects SREBP-1 expression. Thus, ELOVL5 has been established as an SREBP-1 target gene and an important component of the negative feedback loop of de novo lipogenesis. However, the human ELOVL5 promoter/enhancer has not been fully analyzed and the location of SREBP biding sites around the ELOVL5 gene has yet to be defined. Here we performed a detailed promoter/enhancer analysis of human ELOVL5 gene, and identified two new SREBP binding sites, one in the 10 kb upstream region and one in the exon 1. These two SRE motifs are conserved among mammals and the mechanism found in the present study by which SREBP activates ELOVL5 is considered to be common in mammals. Through these findings, we clarified the molecular mechanism how SREBP activates ELOVL5, an important regulator of de novo lipogenesis.

KW - Fatty acid synthesis

KW - Polyunsaturated fatty acids

KW - Transcription

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U2 - 10.1016/j.bbrc.2015.08.101

DO - 10.1016/j.bbrc.2015.08.101

M3 - 学術論文

C2 - 26321664

AN - SCOPUS:84941317250

SN - 0006-291X

VL - 465

SP - 857

EP - 863

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

Identification of human ELOVL5 enhancer regions controlled by SREBP

Abstract

Keywords

ASJC Scopus subject areas

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