Identification of genes responsive to low intensity pulsed ultrasound in a human leukemia cell line Molt-4

Yoshiaki Tabuchi; Hidetaka Ando; Ichiro Takasaki; Loreto B. Feril; Qing Li Zhao; Ryohei Ogawa; Nobuki Kudo; Katsuro Tachibana; Takashi Kondo

doi:10.1016/j.canlet.2006.02.011

Identification of genes responsive to low intensity pulsed ultrasound in a human leukemia cell line Molt-4

Yoshiaki Tabuchi^*, Hidetaka Ando, Ichiro Takasaki, Loreto B. Feril, Qing Li Zhao, Ryohei Ogawa, Nobuki Kudo, Katsuro Tachibana, Takashi Kondo

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

61 Scopus citations

Abstract

We examined the gene expression of human leukemia Molt-4 cells treated with non-thermal low intensity pulsed ultrasound. Six hours after 0.3 W/cm² pulsed ultrasound treatment, apoptosis (24±3.3%, mean±SD) with minimal cell lysis was observed. Of approximately 16,600 genes analyzed, BCL2-associated athanogene 3 (BAG3), DnaJ (Hsp40) homolog, subfamily B, member 1 (DNAJB1), heat shock 70 kDa protein 1B (HSPA1B), and heat shock 70 kDa protein 6 (HSPA6) showed increased levels of expression while isopentenyl-diphosphate delta isomerase (IDI1) and 3-hydroxy-3-methylglutaryl-coenzyme A synthase 1 (HMGCS1) showed decreased levels in the cells 3 h after the ultrasound treatment. The expression levels of these six genes were confirmed by a real-time quantitative polymerase chain reaction. To our knowledge, this is the first report of DNA microarray analysis of genes that are differentially expressed in response to apoptosis induced by non-thermal low intensity pulsed ultrasound in human leukemia cells. The present results will provide a basis for further understanding of the molecular mechanisms of effects of not only low intensity pulsed ultrasound but also that of mechanical shear stress in the cells.

Original language	English
Pages (from-to)	149-156
Number of pages	8
Journal	Cancer Letters
Volume	246
Issue number	1-2
DOIs	https://doi.org/10.1016/j.canlet.2006.02.011
State	Published - 2007/02/08

Keywords

DNA microarray
Gene expression
Low intensity pulsed ultrasound

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.canlet.2006.02.011

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@article{f38cb62f3e24485aa726cd7e94a084f4,

title = "Identification of genes responsive to low intensity pulsed ultrasound in a human leukemia cell line Molt-4",

abstract = "We examined the gene expression of human leukemia Molt-4 cells treated with non-thermal low intensity pulsed ultrasound. Six hours after 0.3 W/cm2 pulsed ultrasound treatment, apoptosis (24±3.3%, mean±SD) with minimal cell lysis was observed. Of approximately 16,600 genes analyzed, BCL2-associated athanogene 3 (BAG3), DnaJ (Hsp40) homolog, subfamily B, member 1 (DNAJB1), heat shock 70 kDa protein 1B (HSPA1B), and heat shock 70 kDa protein 6 (HSPA6) showed increased levels of expression while isopentenyl-diphosphate delta isomerase (IDI1) and 3-hydroxy-3-methylglutaryl-coenzyme A synthase 1 (HMGCS1) showed decreased levels in the cells 3 h after the ultrasound treatment. The expression levels of these six genes were confirmed by a real-time quantitative polymerase chain reaction. To our knowledge, this is the first report of DNA microarray analysis of genes that are differentially expressed in response to apoptosis induced by non-thermal low intensity pulsed ultrasound in human leukemia cells. The present results will provide a basis for further understanding of the molecular mechanisms of effects of not only low intensity pulsed ultrasound but also that of mechanical shear stress in the cells.",

keywords = "DNA microarray, Gene expression, Low intensity pulsed ultrasound",

author = "Yoshiaki Tabuchi and Hidetaka Ando and Ichiro Takasaki and Feril, {Loreto B.} and Zhao, {Qing Li} and Ryohei Ogawa and Nobuki Kudo and Katsuro Tachibana and Takashi Kondo",

note = "Funding Information: The authors express their appreciation to Drs Shin-ichiro Umemura and Hideki Yoshikawa, Central Research Laboratory, Hitachi Co. Ltd, Tokyo, Japan for their support on measurement of peak pressure amplitude of the ultrasonic apparatus. This study is supported in part by the Grant in Aid for Scientific Research on Priority Areas (12217049) from the Ministry of Education, Culture, Sports, Sciences and Technology; in part by the 21st Century COE Program of the Toyama Medical and Pharmaceutical University, Japan; and also in part by Research and Development Committee Program of the Japan Society of Ultrasonics in Medicine.",

year = "2007",

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day = "8",

doi = "10.1016/j.canlet.2006.02.011",

language = "英語",

volume = "246",

pages = "149--156",

journal = "Cancer Letters",

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TY - JOUR

T1 - Identification of genes responsive to low intensity pulsed ultrasound in a human leukemia cell line Molt-4

AU - Tabuchi, Yoshiaki

AU - Ando, Hidetaka

AU - Takasaki, Ichiro

AU - Feril, Loreto B.

AU - Zhao, Qing Li

AU - Ogawa, Ryohei

AU - Kudo, Nobuki

AU - Tachibana, Katsuro

AU - Kondo, Takashi

N1 - Funding Information: The authors express their appreciation to Drs Shin-ichiro Umemura and Hideki Yoshikawa, Central Research Laboratory, Hitachi Co. Ltd, Tokyo, Japan for their support on measurement of peak pressure amplitude of the ultrasonic apparatus. This study is supported in part by the Grant in Aid for Scientific Research on Priority Areas (12217049) from the Ministry of Education, Culture, Sports, Sciences and Technology; in part by the 21st Century COE Program of the Toyama Medical and Pharmaceutical University, Japan; and also in part by Research and Development Committee Program of the Japan Society of Ultrasonics in Medicine.

PY - 2007/2/8

Y1 - 2007/2/8

N2 - We examined the gene expression of human leukemia Molt-4 cells treated with non-thermal low intensity pulsed ultrasound. Six hours after 0.3 W/cm2 pulsed ultrasound treatment, apoptosis (24±3.3%, mean±SD) with minimal cell lysis was observed. Of approximately 16,600 genes analyzed, BCL2-associated athanogene 3 (BAG3), DnaJ (Hsp40) homolog, subfamily B, member 1 (DNAJB1), heat shock 70 kDa protein 1B (HSPA1B), and heat shock 70 kDa protein 6 (HSPA6) showed increased levels of expression while isopentenyl-diphosphate delta isomerase (IDI1) and 3-hydroxy-3-methylglutaryl-coenzyme A synthase 1 (HMGCS1) showed decreased levels in the cells 3 h after the ultrasound treatment. The expression levels of these six genes were confirmed by a real-time quantitative polymerase chain reaction. To our knowledge, this is the first report of DNA microarray analysis of genes that are differentially expressed in response to apoptosis induced by non-thermal low intensity pulsed ultrasound in human leukemia cells. The present results will provide a basis for further understanding of the molecular mechanisms of effects of not only low intensity pulsed ultrasound but also that of mechanical shear stress in the cells.

AB - We examined the gene expression of human leukemia Molt-4 cells treated with non-thermal low intensity pulsed ultrasound. Six hours after 0.3 W/cm2 pulsed ultrasound treatment, apoptosis (24±3.3%, mean±SD) with minimal cell lysis was observed. Of approximately 16,600 genes analyzed, BCL2-associated athanogene 3 (BAG3), DnaJ (Hsp40) homolog, subfamily B, member 1 (DNAJB1), heat shock 70 kDa protein 1B (HSPA1B), and heat shock 70 kDa protein 6 (HSPA6) showed increased levels of expression while isopentenyl-diphosphate delta isomerase (IDI1) and 3-hydroxy-3-methylglutaryl-coenzyme A synthase 1 (HMGCS1) showed decreased levels in the cells 3 h after the ultrasound treatment. The expression levels of these six genes were confirmed by a real-time quantitative polymerase chain reaction. To our knowledge, this is the first report of DNA microarray analysis of genes that are differentially expressed in response to apoptosis induced by non-thermal low intensity pulsed ultrasound in human leukemia cells. The present results will provide a basis for further understanding of the molecular mechanisms of effects of not only low intensity pulsed ultrasound but also that of mechanical shear stress in the cells.

KW - DNA microarray

KW - Gene expression

KW - Low intensity pulsed ultrasound

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U2 - 10.1016/j.canlet.2006.02.011

DO - 10.1016/j.canlet.2006.02.011

M3 - 学術論文

C2 - 16678341

AN - SCOPUS:33845639657

SN - 0304-3835

VL - 246

SP - 149

EP - 156

JO - Cancer Letters

JF - Cancer Letters

IS - 1-2

ER -

Identification of genes responsive to low intensity pulsed ultrasound in a human leukemia cell line Molt-4

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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