Identification of Chemo and Radio-Resistant Sub-Population of Stem Cells in Human Cervical Cancer HeLa Cells

Nabil Mohie Abdel-Hamid; Moustafa Fathy; Chika Koike; Toshiko Yoshida; Motonori Okabe; Kaixuan Zho; Mekky Abouzied; Toshio Nikaido

doi:10.1080/07357907.2021.1931875

Identification of Chemo and Radio-Resistant Sub-Population of Stem Cells in Human Cervical Cancer HeLa Cells

Nabil Mohie Abdel-Hamid^*, Moustafa Fathy, Chika Koike, Toshiko Yoshida, Motonori Okabe, Kaixuan Zho, Mekky Abouzied, Toshio Nikaido

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Background: Cervical cancer ranks the second female malignancy after breast cancer. Cancer stem cells (CSCs) are hard to be eradicated, so can recur. We aim to isolate and characterize CSCs from HeLa cells. Methods: These cells express clusters of differentiation (CDs), 44 and 24, to be sorted by fluorescence-activated cell sorting (FACS). Results: CD44⁺CD24⁺ cells showed potential to form spheres, tumorigenicity, stemness genes and higher resistance to cisplatin, X-ray. Conclusion: CD44⁺CD24⁺ HeLa cells hold characteristics of CSCs, in vitro, in vivo studies, suggesting that targeting may lead to screening of new anti-cancer therapies.

Original language	English
Pages (from-to)	661-674
Number of pages	14
Journal	Cancer Investigation
Volume	39
Issue number	8
DOIs	https://doi.org/10.1080/07357907.2021.1931875
State	Published - 2021

Keywords

CD24
CD44
Cancer stem cells
HeLa cells
chemoresistance
radio-resistance
stemness markers
tumorigenicity

ASJC Scopus subject areas

Oncology
Cancer Research

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10.1080/07357907.2021.1931875

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title = "Identification of Chemo and Radio-Resistant Sub-Population of Stem Cells in Human Cervical Cancer HeLa Cells",

abstract = "Background: Cervical cancer ranks the second female malignancy after breast cancer. Cancer stem cells (CSCs) are hard to be eradicated, so can recur. We aim to isolate and characterize CSCs from HeLa cells. Methods: These cells express clusters of differentiation (CDs), 44 and 24, to be sorted by fluorescence-activated cell sorting (FACS). Results: CD44+CD24+ cells showed potential to form spheres, tumorigenicity, stemness genes and higher resistance to cisplatin, X-ray. Conclusion: CD44+CD24+ HeLa cells hold characteristics of CSCs, in vitro, in vivo studies, suggesting that targeting may lead to screening of new anti-cancer therapies.",

keywords = "CD24, CD44, Cancer stem cells, HeLa cells, chemoresistance, radio-resistance, stemness markers, tumorigenicity",

author = "Abdel-Hamid, {Nabil Mohie} and Moustafa Fathy and Chika Koike and Toshiko Yoshida and Motonori Okabe and Kaixuan Zho and Mekky Abouzied and Toshio Nikaido",

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year = "2021",

doi = "10.1080/07357907.2021.1931875",

language = "英語",

volume = "39",

pages = "661--674",

journal = "Cancer Investigation",

issn = "0735-7907",

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TY - JOUR

T1 - Identification of Chemo and Radio-Resistant Sub-Population of Stem Cells in Human Cervical Cancer HeLa Cells

AU - Abdel-Hamid, Nabil Mohie

AU - Fathy, Moustafa

AU - Koike, Chika

AU - Yoshida, Toshiko

AU - Okabe, Motonori

AU - Zho, Kaixuan

AU - Abouzied, Mekky

AU - Nikaido, Toshio

PY - 2021

Y1 - 2021

N2 - Background: Cervical cancer ranks the second female malignancy after breast cancer. Cancer stem cells (CSCs) are hard to be eradicated, so can recur. We aim to isolate and characterize CSCs from HeLa cells. Methods: These cells express clusters of differentiation (CDs), 44 and 24, to be sorted by fluorescence-activated cell sorting (FACS). Results: CD44+CD24+ cells showed potential to form spheres, tumorigenicity, stemness genes and higher resistance to cisplatin, X-ray. Conclusion: CD44+CD24+ HeLa cells hold characteristics of CSCs, in vitro, in vivo studies, suggesting that targeting may lead to screening of new anti-cancer therapies.

AB - Background: Cervical cancer ranks the second female malignancy after breast cancer. Cancer stem cells (CSCs) are hard to be eradicated, so can recur. We aim to isolate and characterize CSCs from HeLa cells. Methods: These cells express clusters of differentiation (CDs), 44 and 24, to be sorted by fluorescence-activated cell sorting (FACS). Results: CD44+CD24+ cells showed potential to form spheres, tumorigenicity, stemness genes and higher resistance to cisplatin, X-ray. Conclusion: CD44+CD24+ HeLa cells hold characteristics of CSCs, in vitro, in vivo studies, suggesting that targeting may lead to screening of new anti-cancer therapies.

KW - CD24

KW - CD44

KW - Cancer stem cells

KW - HeLa cells

KW - chemoresistance

KW - radio-resistance

KW - stemness markers

KW - tumorigenicity

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DO - 10.1080/07357907.2021.1931875

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SN - 0735-7907

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JF - Cancer Investigation

IS - 8

ER -

Identification of Chemo and Radio-Resistant Sub-Population of Stem Cells in Human Cervical Cancer HeLa Cells

Abstract

Keywords

ASJC Scopus subject areas

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