Identification of cellular proteins interacting with octaarginine (R8) cell-penetrating peptide by photo-crosslinking

Yoshimasa Kawaguchi; Gen Tanaka; Ikuhiko Nakase; Miki Imanishi; Junya Chiba; Yasumaru Hatanaka; Shiroh Futaki

doi:10.1016/j.bmcl.2013.05.008

Identification of cellular proteins interacting with octaarginine (R8) cell-penetrating peptide by photo-crosslinking

Yoshimasa Kawaguchi, Gen Tanaka, Ikuhiko Nakase, Miki Imanishi, Junya Chiba, Yasumaru Hatanaka, Shiroh Futaki^*

^*Corresponding author for this work

Chemical Biology

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Octaarginine (R8) is a representative cell-penetrating peptide. Lanthionine synthetase component C-like protein 1 (LanCL1) was identified as a potential intracellular target of R8 by using a photo-crosslinking assay that utilized a phenyl-trifluoromethyl diazirine moiety and peptide mass fingerprinting. Increased cellular uptake of R8 by LanCL1-overexpressing cells was observed.

Original language	English
Pages (from-to)	3738-3740
Number of pages	3
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	23
Issue number	13
DOIs	https://doi.org/10.1016/j.bmcl.2013.05.008
State	Published - 2013/07/01

Keywords

Arginine-rich cell-penetrating peptide
C-like protein 1
Lanthionine synthetase component
Octaarginine
Phenyl-trifluoromethyl diazirine
Photo-crosslinking

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

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10.1016/j.bmcl.2013.05.008

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title = "Identification of cellular proteins interacting with octaarginine (R8) cell-penetrating peptide by photo-crosslinking",

abstract = "Octaarginine (R8) is a representative cell-penetrating peptide. Lanthionine synthetase component C-like protein 1 (LanCL1) was identified as a potential intracellular target of R8 by using a photo-crosslinking assay that utilized a phenyl-trifluoromethyl diazirine moiety and peptide mass fingerprinting. Increased cellular uptake of R8 by LanCL1-overexpressing cells was observed.",

keywords = "Arginine-rich cell-penetrating peptide, C-like protein 1, Lanthionine synthetase component, Octaarginine, Phenyl-trifluoromethyl diazirine, Photo-crosslinking",

author = "Yoshimasa Kawaguchi and Gen Tanaka and Ikuhiko Nakase and Miki Imanishi and Junya Chiba and Yasumaru Hatanaka and Shiroh Futaki",

note = "Funding Information: This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan . ",

year = "2013",

month = jul,

day = "1",

doi = "10.1016/j.bmcl.2013.05.008",

language = "英語",

volume = "23",

pages = "3738--3740",

journal = "Bioorganic and Medicinal Chemistry Letters",

issn = "0960-894X",

publisher = "Elsevier Ltd.",

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TY - JOUR

T1 - Identification of cellular proteins interacting with octaarginine (R8) cell-penetrating peptide by photo-crosslinking

AU - Kawaguchi, Yoshimasa

AU - Tanaka, Gen

AU - Nakase, Ikuhiko

AU - Imanishi, Miki

AU - Chiba, Junya

AU - Hatanaka, Yasumaru

AU - Futaki, Shiroh

N1 - Funding Information: This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan .

PY - 2013/7/1

Y1 - 2013/7/1

N2 - Octaarginine (R8) is a representative cell-penetrating peptide. Lanthionine synthetase component C-like protein 1 (LanCL1) was identified as a potential intracellular target of R8 by using a photo-crosslinking assay that utilized a phenyl-trifluoromethyl diazirine moiety and peptide mass fingerprinting. Increased cellular uptake of R8 by LanCL1-overexpressing cells was observed.

AB - Octaarginine (R8) is a representative cell-penetrating peptide. Lanthionine synthetase component C-like protein 1 (LanCL1) was identified as a potential intracellular target of R8 by using a photo-crosslinking assay that utilized a phenyl-trifluoromethyl diazirine moiety and peptide mass fingerprinting. Increased cellular uptake of R8 by LanCL1-overexpressing cells was observed.

KW - Arginine-rich cell-penetrating peptide

KW - C-like protein 1

KW - Lanthionine synthetase component

KW - Octaarginine

KW - Phenyl-trifluoromethyl diazirine

KW - Photo-crosslinking

UR - http://www.scopus.com/inward/record.url?scp=84878871991&partnerID=8YFLogxK

U2 - 10.1016/j.bmcl.2013.05.008

DO - 10.1016/j.bmcl.2013.05.008

M3 - 学術論文

C2 - 23726025

AN - SCOPUS:84878871991

SN - 0960-894X

VL - 23

SP - 3738

EP - 3740

JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

IS - 13

ER -

Identification of cellular proteins interacting with octaarginine (R8) cell-penetrating peptide by photo-crosslinking

Abstract

Keywords

ASJC Scopus subject areas

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