TY - JOUR
T1 - Identification of 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin-responsive genes in mouse liver by serial analysis of gene expression
AU - Kurachi, Makoto
AU - Hashimoto, Shin ichi
AU - Obata, Aya
AU - Nagai, Shigenori
AU - Nagahata, Tomoyuki
AU - Matsushima, Kouji
AU - Kurachi, Makoto
AU - Kaneko, Shuichi
AU - Kobayashi, Ken ichi
AU - Inadera, Hidekuni
AU - Sone, Hideko
AU - Tohyama, Chiharu
PY - 2002
Y1 - 2002
N2 - 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD) is one of the most toxic environmental pollutants that causes various biological effects on mammals. To identify the genes involved in hepatotoxicity and hepatocarcinogenesis induced by TCDD, we have conducted here serial analysis of gene expression of mouse liver 7 days after treatment with a single oral dose of 20 μg TCDD/kg body weight. We have sequenced total of 113, 067 tags, including 56, 420 tags and 56, 647 tags from normal liver and TCDD-treated liver library, respectively. Statistical analysis showed that TCDD significantly altered 346 transcripts (p < 0.05) including 94 ESTs. The genes regulated by TCDD were not only the genes encoding drug metabolizing enzymes and stress response genes but also a wide variety of genes encoding cytoskeleton related proteins, signal transduction, and plasma proteins. This comprehensive gene expression analysis would provide novel genes that may help to clarify the mechanism of TCDD effects on mammalian liver, and also give a new approach for prevention and treatment.
AB - 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD) is one of the most toxic environmental pollutants that causes various biological effects on mammals. To identify the genes involved in hepatotoxicity and hepatocarcinogenesis induced by TCDD, we have conducted here serial analysis of gene expression of mouse liver 7 days after treatment with a single oral dose of 20 μg TCDD/kg body weight. We have sequenced total of 113, 067 tags, including 56, 420 tags and 56, 647 tags from normal liver and TCDD-treated liver library, respectively. Statistical analysis showed that TCDD significantly altered 346 transcripts (p < 0.05) including 94 ESTs. The genes regulated by TCDD were not only the genes encoding drug metabolizing enzymes and stress response genes but also a wide variety of genes encoding cytoskeleton related proteins, signal transduction, and plasma proteins. This comprehensive gene expression analysis would provide novel genes that may help to clarify the mechanism of TCDD effects on mammalian liver, and also give a new approach for prevention and treatment.
KW - 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin
KW - Aryl hydrocarbon receptor
KW - Gene expression profile
KW - Mouse liver transcriptome
KW - Serial analysis of gene expression
UR - http://www.scopus.com/inward/record.url?scp=0036295446&partnerID=8YFLogxK
U2 - 10.1006/bbrc.2002.6669
DO - 10.1006/bbrc.2002.6669
M3 - 学術論文
C2 - 11906171
AN - SCOPUS:0036295446
SN - 0006-291X
VL - 292
SP - 368
EP - 377
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -