TY - JOUR
T1 - Hyperdry human amniotic membrane is useful material for tissue engineering
T2 - Physical, morphological properties, and safety as the new biological material
AU - Okabe, Motonori
AU - Kitagawa, Kiyotaka
AU - Yoshida, Toshiko
AU - Suzuki, Takuma
AU - Waki, Hiroki
AU - Koike, Chika
AU - Furuichi, Etsuko
AU - Katou, Kiyoshi
AU - Nomura, Yoshihiro
AU - Uji, Yoshinori
AU - Hayashi, Atsushi
AU - Saito, Shigeru
AU - Nikaido, Toshio
PY - 2014/3
Y1 - 2014/3
N2 - Human amniotic membrane (AM) has been used widely as graft biomaterial for a variety of clinical applications. But, there are some persistent problems related to the preparation, storage, and sterilization. To resolve these problems, we developed hyperdry AM (HD-AM) using far-infrared rays, depression of air, and microwaves and then sterilized by γ-ray irradiation. To elucidate the benefit of HD-AM as biological materials, compare with the physical and histological properties of HD-AM with a freeze-dried AM (FD-AM) as typical freeze-dried methods, evaluate the safety of HD-AM in vivo experiment used nude mice, and demonstrate the feasibility of HD-AM transplant in pterygium. The water permeability and the sieving coefficient of HD-AM were significantly lower than that of FD-AM. HD-AM has kept the morphological structure of epithelium and connective tissues. At 18 months after transplanted, single and multilayers of HD-AM in the intraperitoneal cavity was degraded without any infiltrated cells. For clinical treatment, recurrence of pterygium and regrowth of the subconjunctival fibrosis were not observed during the 6-month follow-up periods after the surgery. It was proposed that HD-AM was a safe and effective new biological material for clinical use including treatment for recurrent pterygium. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 862-870, 2014.
AB - Human amniotic membrane (AM) has been used widely as graft biomaterial for a variety of clinical applications. But, there are some persistent problems related to the preparation, storage, and sterilization. To resolve these problems, we developed hyperdry AM (HD-AM) using far-infrared rays, depression of air, and microwaves and then sterilized by γ-ray irradiation. To elucidate the benefit of HD-AM as biological materials, compare with the physical and histological properties of HD-AM with a freeze-dried AM (FD-AM) as typical freeze-dried methods, evaluate the safety of HD-AM in vivo experiment used nude mice, and demonstrate the feasibility of HD-AM transplant in pterygium. The water permeability and the sieving coefficient of HD-AM were significantly lower than that of FD-AM. HD-AM has kept the morphological structure of epithelium and connective tissues. At 18 months after transplanted, single and multilayers of HD-AM in the intraperitoneal cavity was degraded without any infiltrated cells. For clinical treatment, recurrence of pterygium and regrowth of the subconjunctival fibrosis were not observed during the 6-month follow-up periods after the surgery. It was proposed that HD-AM was a safe and effective new biological material for clinical use including treatment for recurrent pterygium. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 862-870, 2014.
KW - biological material
KW - clinical use
KW - freeze-dried human amniotic membrane
KW - hyperdry human amniotic membrane
KW - recurrent pterygium
UR - http://www.scopus.com/inward/record.url?scp=84892569870&partnerID=8YFLogxK
U2 - 10.1002/jbm.a.34753
DO - 10.1002/jbm.a.34753
M3 - 学術論文
C2 - 23589398
AN - SCOPUS:84892569870
SN - 1549-3296
VL - 102
SP - 862
EP - 870
JO - Journal of Biomedical Materials Research - Part A
JF - Journal of Biomedical Materials Research - Part A
IS - 3
ER -