HSP90 has neurite-promoting activity in vitro for telencephalic and spinal neurons of chick embryos

Tetsuya Ishimoto; Akihito Kamei; Satoshi Koyanagi; Naoki Nishide; Atsuko Uyeda; Michiki Kasai; Takahisa Taguchi

doi:10.1006/bbrc.1998.9701

HSP90 has neurite-promoting activity in vitro for telencephalic and spinal neurons of chick embryos

Tetsuya Ishimoto, Akihito Kamei, Satoshi Koyanagi, Naoki Nishide, Atsuko Uyeda, Michiki Kasai, Takahisa Taguchi^*

^*Corresponding author for this work

Molecular Neuroscience

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

We purified a protein from the extract of denervated chick muscle. The protein had neurite-promoting activity in vitro for the chick telencephalic neurons and spinal neurons. Partial amino acid sequencing and immunoblotting revealed that the protein was chick heat shock protein 90 (HSP90). Commercially available bovine HSP90 and recombinant chick HSP90 expressed in Escherichia coli also showed the same activity. Since HSP10 (GroES) and HSP60 (GroEL) exhibited no activity for neurite outgrowth in the same culture, this activity was specific to HSP90 among the heat shock proteins.

Original language	English
Pages (from-to)	283-287
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	253
Issue number	2
DOIs	https://doi.org/10.1006/bbrc.1998.9701
State	Published - 1998/12/18

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "HSP90 has neurite-promoting activity in vitro for telencephalic and spinal neurons of chick embryos",

abstract = "We purified a protein from the extract of denervated chick muscle. The protein had neurite-promoting activity in vitro for the chick telencephalic neurons and spinal neurons. Partial amino acid sequencing and immunoblotting revealed that the protein was chick heat shock protein 90 (HSP90). Commercially available bovine HSP90 and recombinant chick HSP90 expressed in Escherichia coli also showed the same activity. Since HSP10 (GroES) and HSP60 (GroEL) exhibited no activity for neurite outgrowth in the same culture, this activity was specific to HSP90 among the heat shock proteins.",

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AU - Ishimoto, Tetsuya

AU - Kamei, Akihito

AU - Koyanagi, Satoshi

AU - Nishide, Naoki

AU - Uyeda, Atsuko

AU - Kasai, Michiki

AU - Taguchi, Takahisa

PY - 1998/12/18

Y1 - 1998/12/18

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AB - We purified a protein from the extract of denervated chick muscle. The protein had neurite-promoting activity in vitro for the chick telencephalic neurons and spinal neurons. Partial amino acid sequencing and immunoblotting revealed that the protein was chick heat shock protein 90 (HSP90). Commercially available bovine HSP90 and recombinant chick HSP90 expressed in Escherichia coli also showed the same activity. Since HSP10 (GroES) and HSP60 (GroEL) exhibited no activity for neurite outgrowth in the same culture, this activity was specific to HSP90 among the heat shock proteins.

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HSP90 has neurite-promoting activity in vitro for telencephalic and spinal neurons of chick embryos

Abstract

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