High-resolution label-free 3D mapping of extracellular pH of single living cells

Yanjun Zhang; Yasufumi Takahashi; Sung Pil Hong; Fengjie Liu; Joanna Bednarska; Philip S. Goff; Pavel Novak; Andrew Shevchuk; Sahana Gopal; Iros Barozzi; Luca Magnani; Hideki Sakai; Yoshimoto Suguru; Takuto Fujii; Alexander Erofeev; Peter Gorelkin; Alexander Majouga; Dominik J. Weiss; Christopher Edwards; Aleksandar P. Ivanov; David Klenerman; Elena V. Sviderskaya; Joshua B. Edel; Yuri Korchev

doi:10.1038/s41467-019-13535-1

High-resolution label-free 3D mapping of extracellular pH of single living cells

Yanjun Zhang^*, Yasufumi Takahashi, Sung Pil Hong, Fengjie Liu, Joanna Bednarska, Philip S. Goff, Pavel Novak, Andrew Shevchuk, Sahana Gopal, Iros Barozzi, Luca Magnani, Hideki Sakai, Yoshimoto Suguru, Takuto Fujii, Alexander Erofeev, Peter Gorelkin, Alexander Majouga, Dominik J. Weiss, Christopher Edwards, Aleksandar P. IvanovDavid Klenerman, Elena V. Sviderskaya, Joshua B. Edel, Yuri Korchev

^*Corresponding author for this work

Pharmaceutical Physiology

Research output: Contribution to journal › Article › peer-review

81 Scopus citations

Abstract

Dynamic mapping of extracellular pH (pHe) at the single-cell level is critical for understanding the role of H⁺ in cellular and subcellular processes, with particular importance in cancer. While several pHe sensing techniques have been developed, accessing this information at the single-cell level requires improvement in sensitivity, spatial and temporal resolution. We report on a zwitterionic label-free pH nanoprobe that addresses these long-standing challenges. The probe has a sensitivity > 0.01 units, 2 ms response time, and 50 nm spatial resolution. The platform was integrated into a double-barrel nanoprobe combining pH sensing with feedback-controlled distance dependance via Scanning Ion Conductance Microscopy. This allows for the simultaneous 3D topographical imaging and pHe monitoring of living cancer cells. These classes of nanoprobes were used for real-time high spatiotemporal resolution pHe mapping at the subcellular level and revealed tumour heterogeneity of the peri-cellular environments of melanoma and breast cancer cells.

Original language	English
Article number	5610
Journal	Nature Communications
Volume	10
Issue number	1
DOIs	https://doi.org/10.1038/s41467-019-13535-1
State	Published - 2019/12/01

ASJC Scopus subject areas

General Chemistry
General Biochemistry, Genetics and Molecular Biology
General Physics and Astronomy

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Zhang, Y., Takahashi, Y., Hong, S. P., Liu, F., Bednarska, J., Goff, P. S., Novak, P., Shevchuk, A., Gopal, S., Barozzi, I., Magnani, L., Sakai, H., Suguru, Y., Fujii, T., Erofeev, A., Gorelkin, P., Majouga, A., Weiss, D. J., Edwards, C., ... Korchev, Y. (2019). High-resolution label-free 3D mapping of extracellular pH of single living cells. Nature Communications, 10(1), Article 5610. https://doi.org/10.1038/s41467-019-13535-1

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title = "High-resolution label-free 3D mapping of extracellular pH of single living cells",

abstract = "Dynamic mapping of extracellular pH (pHe) at the single-cell level is critical for understanding the role of H+ in cellular and subcellular processes, with particular importance in cancer. While several pHe sensing techniques have been developed, accessing this information at the single-cell level requires improvement in sensitivity, spatial and temporal resolution. We report on a zwitterionic label-free pH nanoprobe that addresses these long-standing challenges. The probe has a sensitivity > 0.01 units, 2 ms response time, and 50 nm spatial resolution. The platform was integrated into a double-barrel nanoprobe combining pH sensing with feedback-controlled distance dependance via Scanning Ion Conductance Microscopy. This allows for the simultaneous 3D topographical imaging and pHe monitoring of living cancer cells. These classes of nanoprobes were used for real-time high spatiotemporal resolution pHe mapping at the subcellular level and revealed tumour heterogeneity of the peri-cellular environments of melanoma and breast cancer cells.",

author = "Yanjun Zhang and Yasufumi Takahashi and Hong, {Sung Pil} and Fengjie Liu and Joanna Bednarska and Goff, {Philip S.} and Pavel Novak and Andrew Shevchuk and Sahana Gopal and Iros Barozzi and Luca Magnani and Hideki Sakai and Yoshimoto Suguru and Takuto Fujii and Alexander Erofeev and Peter Gorelkin and Alexander Majouga and Weiss, {Dominik J.} and Christopher Edwards and Ivanov, {Aleksandar P.} and David Klenerman and Sviderskaya, {Elena V.} and Edel, {Joshua B.} and Yuri Korchev",

note = "Publisher Copyright: {\textcopyright} 2019, The Author(s).",

year = "2019",

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doi = "10.1038/s41467-019-13535-1",

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Zhang, Y, Takahashi, Y, Hong, SP, Liu, F, Bednarska, J, Goff, PS, Novak, P, Shevchuk, A, Gopal, S, Barozzi, I, Magnani, L, Sakai, H, Suguru, Y, Fujii, T, Erofeev, A, Gorelkin, P, Majouga, A, Weiss, DJ, Edwards, C, Ivanov, AP, Klenerman, D, Sviderskaya, EV, Edel, JB & Korchev, Y 2019, 'High-resolution label-free 3D mapping of extracellular pH of single living cells', Nature Communications, vol. 10, no. 1, 5610. https://doi.org/10.1038/s41467-019-13535-1

TY - JOUR

T1 - High-resolution label-free 3D mapping of extracellular pH of single living cells

AU - Zhang, Yanjun

AU - Takahashi, Yasufumi

AU - Hong, Sung Pil

AU - Liu, Fengjie

AU - Bednarska, Joanna

AU - Goff, Philip S.

AU - Novak, Pavel

AU - Shevchuk, Andrew

AU - Gopal, Sahana

AU - Barozzi, Iros

AU - Magnani, Luca

AU - Sakai, Hideki

AU - Suguru, Yoshimoto

AU - Fujii, Takuto

AU - Erofeev, Alexander

AU - Gorelkin, Peter

AU - Majouga, Alexander

AU - Weiss, Dominik J.

AU - Edwards, Christopher

AU - Ivanov, Aleksandar P.

AU - Klenerman, David

AU - Sviderskaya, Elena V.

AU - Edel, Joshua B.

AU - Korchev, Yuri

PY - 2019/12/1

Y1 - 2019/12/1

N2 - Dynamic mapping of extracellular pH (pHe) at the single-cell level is critical for understanding the role of H+ in cellular and subcellular processes, with particular importance in cancer. While several pHe sensing techniques have been developed, accessing this information at the single-cell level requires improvement in sensitivity, spatial and temporal resolution. We report on a zwitterionic label-free pH nanoprobe that addresses these long-standing challenges. The probe has a sensitivity > 0.01 units, 2 ms response time, and 50 nm spatial resolution. The platform was integrated into a double-barrel nanoprobe combining pH sensing with feedback-controlled distance dependance via Scanning Ion Conductance Microscopy. This allows for the simultaneous 3D topographical imaging and pHe monitoring of living cancer cells. These classes of nanoprobes were used for real-time high spatiotemporal resolution pHe mapping at the subcellular level and revealed tumour heterogeneity of the peri-cellular environments of melanoma and breast cancer cells.

AB - Dynamic mapping of extracellular pH (pHe) at the single-cell level is critical for understanding the role of H+ in cellular and subcellular processes, with particular importance in cancer. While several pHe sensing techniques have been developed, accessing this information at the single-cell level requires improvement in sensitivity, spatial and temporal resolution. We report on a zwitterionic label-free pH nanoprobe that addresses these long-standing challenges. The probe has a sensitivity > 0.01 units, 2 ms response time, and 50 nm spatial resolution. The platform was integrated into a double-barrel nanoprobe combining pH sensing with feedback-controlled distance dependance via Scanning Ion Conductance Microscopy. This allows for the simultaneous 3D topographical imaging and pHe monitoring of living cancer cells. These classes of nanoprobes were used for real-time high spatiotemporal resolution pHe mapping at the subcellular level and revealed tumour heterogeneity of the peri-cellular environments of melanoma and breast cancer cells.

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DO - 10.1038/s41467-019-13535-1

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AN - SCOPUS:85076310223

SN - 2041-1723

VL - 10

JO - Nature Communications

JF - Nature Communications

IS - 1

M1 - 5610

ER -

High-resolution label-free 3D mapping of extracellular pH of single living cells

Abstract

ASJC Scopus subject areas

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