TY - JOUR
T1 - Heterogeneity of the atp6 presequences in normal and different sources of male-sterile cytoplasms of sugar beet
AU - Onodera, Yasuyuki
AU - Yamamoto, Masayuki P.
AU - Kubo, Tomohiko
AU - Mikami, Tetsuo
N1 - Funding Information:
We gratefully acknowledge Drs. H. Kimura and M. Senda for valuable suggestions, and T. Sakai and M. Ushioda for technical assistance. This work was done in part at the Research Center for Molecular Genetics, Hokkaido University, and was supported in part by Grants in Aid for Scientific Research from the Ministry of Education, Science and Culture, Japan and a Grant from the Program for Promotion of Basic Research Activities for Innovative Biosciences, Japan. Y. O. and M. P. Y. are the recipients of the JSPS Fellowship for Japanese Junior Scientists.
PY - 1999/10
Y1 - 1999/10
N2 - We have characterized the mitochondrial atp6 loci from male-fertile (TK81-O), Owen CMS (TK81MS), and wild beet-derived CMS (I-12CMS (3)) cytoplasms of sugar beet (Beta vulgaris L.). The TK81-O atp6 lacks a presequence characteristic of the atp6 genes from a variety of organisms. Interestingly, RNA editing was found to create an initiation codon in TK81-O atp6 mRNA, resulting in a 5 amino acid presequence. On the other hand, TK81-MS atp6 and I-12CMS (3) atp6 have presequences of 387 and 389 residues, respectively. Western blot analysis failed to reveal polymorphisms in size and abundance of the accumulated ATP6 polypeptide among the three genotypes. Our results thus favour the view that the presequence is proteolytically discarded upon maturation of the gene product and that atp6 itself is not the cause of the sterility phenotype.
AB - We have characterized the mitochondrial atp6 loci from male-fertile (TK81-O), Owen CMS (TK81MS), and wild beet-derived CMS (I-12CMS (3)) cytoplasms of sugar beet (Beta vulgaris L.). The TK81-O atp6 lacks a presequence characteristic of the atp6 genes from a variety of organisms. Interestingly, RNA editing was found to create an initiation codon in TK81-O atp6 mRNA, resulting in a 5 amino acid presequence. On the other hand, TK81-MS atp6 and I-12CMS (3) atp6 have presequences of 387 and 389 residues, respectively. Western blot analysis failed to reveal polymorphisms in size and abundance of the accumulated ATP6 polypeptide among the three genotypes. Our results thus favour the view that the presequence is proteolytically discarded upon maturation of the gene product and that atp6 itself is not the cause of the sterility phenotype.
KW - Anti-ATP6 antibody
KW - Atp6
KW - Beta vulgaris
KW - Cytoplasmic male-sterility
UR - http://www.scopus.com/inward/record.url?scp=0032697926&partnerID=8YFLogxK
U2 - 10.1016/S0176-1617(99)80069-8
DO - 10.1016/S0176-1617(99)80069-8
M3 - 学術論文
AN - SCOPUS:0032697926
SN - 0176-1617
VL - 155
SP - 656
EP - 660
JO - Journal of Plant Physiology
JF - Journal of Plant Physiology
IS - 4-5
ER -