Glucose sensing by a carbon-paste electrode containing chitin modified with glucose oxidase

Kazuharu Sugawara; Tomohiro Takano; Hiroshi Fukushi; Suwaru Hoshi; Kunihiko Akatsuka; Hideki Kuramitz; Shunitz Tanaka

doi:10.1016/S0022-0728(00)00004-8

Glucose sensing by a carbon-paste electrode containing chitin modified with glucose oxidase

Kazuharu Sugawara, Tomohiro Takano, Hiroshi Fukushi, Suwaru Hoshi, Kunihiko Akatsuka, Hideki Kuramitz, Shunitz Tanaka

Department of Natural and Environmental Science

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

As one application for chitin, the development of a glucose sensor was attempted at a carbon paste electrode containing platinum powder and chitin powder with glucose oxidase. Immobilization of the enzyme is based on an electrostatic interaction between the protonated acetylamide group of the chitin and functional groups having a negative charge on the enzyme. Glucose oxidase was simply immobilized on the chitin in 0.1 M acetate buffer (pH 5.0). An oxidation peak of H₂O₂ produced from the enzyme reaction was observed at about +0.60 V (vs. Ag | AgCl) in 0.1 M acetate buffer (pH 6.2). The calibration curve of glucose was linear in the range from 3 × 10^-6 to 4 × 10^-4 M. This method was applied to determination of glucose in a sports drink.

Original language	English
Pages (from-to)	81-86
Number of pages	6
Journal	Journal of Electroanalytical Chemistry
Volume	482
Issue number	1
DOIs	https://doi.org/10.1016/S0022-0728(00)00004-8
State	Published - 2000/02/29

ASJC Scopus subject areas

Analytical Chemistry
General Chemical Engineering
Electrochemistry

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10.1016/S0022-0728(00)00004-8

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title = "Glucose sensing by a carbon-paste electrode containing chitin modified with glucose oxidase",

abstract = "As one application for chitin, the development of a glucose sensor was attempted at a carbon paste electrode containing platinum powder and chitin powder with glucose oxidase. Immobilization of the enzyme is based on an electrostatic interaction between the protonated acetylamide group of the chitin and functional groups having a negative charge on the enzyme. Glucose oxidase was simply immobilized on the chitin in 0.1 M acetate buffer (pH 5.0). An oxidation peak of H2O2 produced from the enzyme reaction was observed at about +0.60 V (vs. Ag | AgCl) in 0.1 M acetate buffer (pH 6.2). The calibration curve of glucose was linear in the range from 3 × 10-6 to 4 × 10-4 M. This method was applied to determination of glucose in a sports drink.",

author = "Kazuharu Sugawara and Tomohiro Takano and Hiroshi Fukushi and Suwaru Hoshi and Kunihiko Akatsuka and Hideki Kuramitz and Shunitz Tanaka",

note = "Funding Information: The authors thank the Ministry of Education, Science and Culture of Japan for support of this work under a Grant-in-Aid for Scientific Research (no. 09740548).",

year = "2000",

month = feb,

day = "29",

doi = "10.1016/S0022-0728(00)00004-8",

language = "英語",

volume = "482",

pages = "81--86",

journal = "Journal of Electroanalytical Chemistry",

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TY - JOUR

T1 - Glucose sensing by a carbon-paste electrode containing chitin modified with glucose oxidase

AU - Sugawara, Kazuharu

AU - Takano, Tomohiro

AU - Fukushi, Hiroshi

AU - Hoshi, Suwaru

AU - Akatsuka, Kunihiko

AU - Kuramitz, Hideki

AU - Tanaka, Shunitz

N1 - Funding Information: The authors thank the Ministry of Education, Science and Culture of Japan for support of this work under a Grant-in-Aid for Scientific Research (no. 09740548).

PY - 2000/2/29

Y1 - 2000/2/29

N2 - As one application for chitin, the development of a glucose sensor was attempted at a carbon paste electrode containing platinum powder and chitin powder with glucose oxidase. Immobilization of the enzyme is based on an electrostatic interaction between the protonated acetylamide group of the chitin and functional groups having a negative charge on the enzyme. Glucose oxidase was simply immobilized on the chitin in 0.1 M acetate buffer (pH 5.0). An oxidation peak of H2O2 produced from the enzyme reaction was observed at about +0.60 V (vs. Ag | AgCl) in 0.1 M acetate buffer (pH 6.2). The calibration curve of glucose was linear in the range from 3 × 10-6 to 4 × 10-4 M. This method was applied to determination of glucose in a sports drink.

AB - As one application for chitin, the development of a glucose sensor was attempted at a carbon paste electrode containing platinum powder and chitin powder with glucose oxidase. Immobilization of the enzyme is based on an electrostatic interaction between the protonated acetylamide group of the chitin and functional groups having a negative charge on the enzyme. Glucose oxidase was simply immobilized on the chitin in 0.1 M acetate buffer (pH 5.0). An oxidation peak of H2O2 produced from the enzyme reaction was observed at about +0.60 V (vs. Ag | AgCl) in 0.1 M acetate buffer (pH 6.2). The calibration curve of glucose was linear in the range from 3 × 10-6 to 4 × 10-4 M. This method was applied to determination of glucose in a sports drink.

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U2 - 10.1016/S0022-0728(00)00004-8

DO - 10.1016/S0022-0728(00)00004-8

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SN - 0022-0728

VL - 482

SP - 81

EP - 86

JO - Journal of Electroanalytical Chemistry

JF - Journal of Electroanalytical Chemistry

IS - 1

ER -

Glucose sensing by a carbon-paste electrode containing chitin modified with glucose oxidase

Abstract

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