Genetic networks responsive to low-intensity pulsed ultrasound in human lymphoma U937 cells

Yoshiaki Tabuchi; Ichiro Takasaki; Qing Li Zhao; Shigehito Wada; Takeshi Hori; Loreto B. Feril; Katsuro Tachibana; Takaharu Nomura; Takashi Kondo

doi:10.1016/j.canlet.2008.05.018

Genetic networks responsive to low-intensity pulsed ultrasound in human lymphoma U937 cells

Yoshiaki Tabuchi^*, Ichiro Takasaki, Qing Li Zhao, Shigehito Wada, Takeshi Hori, Loreto B. Feril, Katsuro Tachibana, Takaharu Nomura, Takashi Kondo

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

To clarify the detailed molecular mechanism underlying cellular responses to nonthermal low-intensity pulsed ultrasound (LIPUS), gene expression patterns and genetic networks in human lymphoma U937 cells were examined using global-scale microarrays and computational gene expression analysis tools. Six hours after LIPUS treatment (0.3 W/cm² for 1 min), apoptosis (14 ± 3.8%, mean ± SD) without no cell lysis was observed. Of 22,283 probe sets analyzed, LIPUS down-regulated 193 genes and up-regulated 201 genes by >1.5-fold. For down-regulated genes, the significant genetic network D was associated with cellular growth and proliferation, gene expression, or cellular development. For up-regulated genes, the significant genetic network U was associated with cellular movement, cell morphology, and cell death. The present results indicate that LIPUS affects the expression of many genes and will provide novel insight into the biomolecular mechanisms of LIPUS in therapeutic application for cancer therapy.

Original language	English
Pages (from-to)	286-294
Number of pages	9
Journal	Cancer Letters
Volume	270
Issue number	2
DOIs	https://doi.org/10.1016/j.canlet.2008.05.018
State	Published - 2008/11/08

Keywords

Apoptosis
Gene expression
Genetic network
Human lymphoma U937 cells
Low-intensity pulsed ultrasound

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.canlet.2008.05.018

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@article{19595de86808444a90512f71cfffb6b6,

title = "Genetic networks responsive to low-intensity pulsed ultrasound in human lymphoma U937 cells",

abstract = "To clarify the detailed molecular mechanism underlying cellular responses to nonthermal low-intensity pulsed ultrasound (LIPUS), gene expression patterns and genetic networks in human lymphoma U937 cells were examined using global-scale microarrays and computational gene expression analysis tools. Six hours after LIPUS treatment (0.3 W/cm2 for 1 min), apoptosis (14 ± 3.8%, mean ± SD) without no cell lysis was observed. Of 22,283 probe sets analyzed, LIPUS down-regulated 193 genes and up-regulated 201 genes by >1.5-fold. For down-regulated genes, the significant genetic network D was associated with cellular growth and proliferation, gene expression, or cellular development. For up-regulated genes, the significant genetic network U was associated with cellular movement, cell morphology, and cell death. The present results indicate that LIPUS affects the expression of many genes and will provide novel insight into the biomolecular mechanisms of LIPUS in therapeutic application for cancer therapy.",

keywords = "Apoptosis, Gene expression, Genetic network, Human lymphoma U937 cells, Low-intensity pulsed ultrasound",

author = "Yoshiaki Tabuchi and Ichiro Takasaki and Zhao, {Qing Li} and Shigehito Wada and Takeshi Hori and Feril, {Loreto B.} and Katsuro Tachibana and Takaharu Nomura and Takashi Kondo",

note = "Funding Information: This study was supported in part by a Grant-in-Aid for Scientific Research 17310032 and 19310040 from Japanese Ministry of Education, Culture, Sports, Science, and Technology. ",

year = "2008",

month = nov,

day = "8",

doi = "10.1016/j.canlet.2008.05.018",

language = "英語",

volume = "270",

pages = "286--294",

journal = "Cancer Letters",

issn = "0304-3835",

publisher = "Elsevier Ireland Ltd",

number = "2",

}

TY - JOUR

T1 - Genetic networks responsive to low-intensity pulsed ultrasound in human lymphoma U937 cells

AU - Tabuchi, Yoshiaki

AU - Takasaki, Ichiro

AU - Zhao, Qing Li

AU - Wada, Shigehito

AU - Hori, Takeshi

AU - Feril, Loreto B.

AU - Tachibana, Katsuro

AU - Nomura, Takaharu

AU - Kondo, Takashi

N1 - Funding Information: This study was supported in part by a Grant-in-Aid for Scientific Research 17310032 and 19310040 from Japanese Ministry of Education, Culture, Sports, Science, and Technology.

PY - 2008/11/8

Y1 - 2008/11/8

N2 - To clarify the detailed molecular mechanism underlying cellular responses to nonthermal low-intensity pulsed ultrasound (LIPUS), gene expression patterns and genetic networks in human lymphoma U937 cells were examined using global-scale microarrays and computational gene expression analysis tools. Six hours after LIPUS treatment (0.3 W/cm2 for 1 min), apoptosis (14 ± 3.8%, mean ± SD) without no cell lysis was observed. Of 22,283 probe sets analyzed, LIPUS down-regulated 193 genes and up-regulated 201 genes by >1.5-fold. For down-regulated genes, the significant genetic network D was associated with cellular growth and proliferation, gene expression, or cellular development. For up-regulated genes, the significant genetic network U was associated with cellular movement, cell morphology, and cell death. The present results indicate that LIPUS affects the expression of many genes and will provide novel insight into the biomolecular mechanisms of LIPUS in therapeutic application for cancer therapy.

AB - To clarify the detailed molecular mechanism underlying cellular responses to nonthermal low-intensity pulsed ultrasound (LIPUS), gene expression patterns and genetic networks in human lymphoma U937 cells were examined using global-scale microarrays and computational gene expression analysis tools. Six hours after LIPUS treatment (0.3 W/cm2 for 1 min), apoptosis (14 ± 3.8%, mean ± SD) without no cell lysis was observed. Of 22,283 probe sets analyzed, LIPUS down-regulated 193 genes and up-regulated 201 genes by >1.5-fold. For down-regulated genes, the significant genetic network D was associated with cellular growth and proliferation, gene expression, or cellular development. For up-regulated genes, the significant genetic network U was associated with cellular movement, cell morphology, and cell death. The present results indicate that LIPUS affects the expression of many genes and will provide novel insight into the biomolecular mechanisms of LIPUS in therapeutic application for cancer therapy.

KW - Apoptosis

KW - Gene expression

KW - Genetic network

KW - Human lymphoma U937 cells

KW - Low-intensity pulsed ultrasound

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U2 - 10.1016/j.canlet.2008.05.018

DO - 10.1016/j.canlet.2008.05.018

M3 - 学術論文

C2 - 18571840

AN - SCOPUS:52149102271

SN - 0304-3835

VL - 270

SP - 286

EP - 294

JO - Cancer Letters

JF - Cancer Letters

IS - 2

ER -

Genetic networks responsive to low-intensity pulsed ultrasound in human lymphoma U937 cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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