TY - JOUR
T1 - Gene expression and physiologic responses of the heart to the initiation and withdrawal of caloric restriction
AU - Dhahbi, Joseph M.
AU - Tsuchiya, Tomoshi
AU - Kim, Hyon Jeen
AU - Mote, Patricia L.
AU - Spindler, Stephen R.
N1 - Funding Information:
ACKNOWLEDGMENTS This work was supported by unrestricted gifts from the Life Extension Foundation. Dr. Dhahbi is now with BioMarker Pharmaceuticals, Inc., Alameda, CA. Dr. Tsuchiya is now with the Department of Pathology & Gerontology, Nagasaki University Graduate School of Biomedical Science, Japan. Dr. Kim is now with the School of Medicine, University of California, Los Angeles. Address correspondence to Stephen R. Spindler, PhD, Department of Biochemistry, University of California–Riverside, 3401 Watkins Dr., Riverside, CA 92521. E-mail: spindler@ucr.edu REFERENCES 1. Weisfeldt M. Aging, changes in the cardiovascular system, and responses to stress. Am J Hypertens. 1998;11:41S–45S.
PY - 2006/3
Y1 - 2006/3
N2 - Aging increases and caloric restriction (CR) decreases morbidity and mortality associated with the cardiovascular system. Using Affymetrix microarrays, we identified changes in heart gene expression induced by aging and CR in male mice. Eight weeks of CR (CR8) reproduced 19% of the long-term CR (LTCR)-related expression changes. Because CR8 begins to extend the life span of these mice, these genes may be keys to its cardioprotective effects. CR8 and LTCR changed gene expression in a manner consistent with reduced remodeling and fibrosis, and enhanced contractility and energy production via lipid β-oxidation. Molecular and histochemical studies indicated that CR reduced natriuretic peptide precursor type B and collagen expression, and reduced perivascular collagen deposition. We found smaller cardiomyocytes in the left ventricle of old-LTCR mice, suggesting reduced age-related cell death. Eight weeks of control feeding returned 97% of the LTCR-responsive genes to control expression levels. Thus, key CR-induced effects are rapidly responsive to diet, suggesting reduced caloric intake has rapid, positive effects on the heart.
AB - Aging increases and caloric restriction (CR) decreases morbidity and mortality associated with the cardiovascular system. Using Affymetrix microarrays, we identified changes in heart gene expression induced by aging and CR in male mice. Eight weeks of CR (CR8) reproduced 19% of the long-term CR (LTCR)-related expression changes. Because CR8 begins to extend the life span of these mice, these genes may be keys to its cardioprotective effects. CR8 and LTCR changed gene expression in a manner consistent with reduced remodeling and fibrosis, and enhanced contractility and energy production via lipid β-oxidation. Molecular and histochemical studies indicated that CR reduced natriuretic peptide precursor type B and collagen expression, and reduced perivascular collagen deposition. We found smaller cardiomyocytes in the left ventricle of old-LTCR mice, suggesting reduced age-related cell death. Eight weeks of control feeding returned 97% of the LTCR-responsive genes to control expression levels. Thus, key CR-induced effects are rapidly responsive to diet, suggesting reduced caloric intake has rapid, positive effects on the heart.
UR - http://www.scopus.com/inward/record.url?scp=33645987888&partnerID=8YFLogxK
U2 - 10.1093/gerona/61.3.218
DO - 10.1093/gerona/61.3.218
M3 - 学術論文
C2 - 16567370
AN - SCOPUS:33645987888
SN - 1079-5006
VL - 61
SP - 218
EP - 231
JO - Journals of Gerontology - Series A Biological Sciences and Medical Sciences
JF - Journals of Gerontology - Series A Biological Sciences and Medical Sciences
IS - 3
ER -