Functional evidence for an apical ANP receptor in LLC-PK1 kidney epithelial cells

Hashimoto Yukiya Hashimoto; Ozaki Junko Ozaki; Yasuhara Masato Yasuhara; Hori Ryohei Hori; Suga Shin-ichi Suga; Itoh Hiroshi Itoh; Nakao Kazuwa Nakao; Inui Ken-inchi Inui

doi:10.1016/0922-4106(94)90071-X

Functional evidence for an apical ANP receptor in LLC-PK₁ kidney epithelial cells

Hashimoto Yukiya Hashimoto, Ozaki Junko Ozaki, Yasuhara Masato Yasuhara, Hori Ryohei Hori, Suga Shin-ichi Suga, Itoh Hiroshi Itoh, Nakao Kazuwa Nakao, Inui Ken-inchi Inui^*

^*Corresponding author for this work

Clinical Pharmacokinetics

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

The localization of natriuretic peptide receptors was studied in LLC-PK₁ cell monolayers grown on a micro-porous membrane filter. The addition of α-human atrial natriuretic peptide (α-hANP) to the apical side of the cells produced a concentration-dependent increase in cyclic GMP (cGMP) formation, the extent of which was similar to that produced by the addition to the basolateral side. More cGMP was formed with α-hANP (1 and 100 nM) than with the C-type natriuretic peptide (100 nM). These findings indicate that the biologically active receptors with high efficacy for α-hANP are expressed in both the apical and basolateral membranes in LLC-PK₁ cells.

Original language	English
Pages (from-to)	443-445
Number of pages	3
Journal	European Journal of Pharmacology: Molecular Pharmacology
Volume	268
Issue number	3
DOIs	https://doi.org/10.1016/0922-4106(94)90071-X
State	Published - 1994/08/16

Keywords

ANP (atrial natriuretic peptide)
Guanylate cyclase
LLC-PK cell
Natriuretic peptide, C-type

ASJC Scopus subject areas

Pharmacology

Access to Document

10.1016/0922-4106(94)90071-X

Cite this

@article{e9240d2b64d446cabe84126b075e5bdd,

title = "Functional evidence for an apical ANP receptor in LLC-PK1 kidney epithelial cells",

abstract = "The localization of natriuretic peptide receptors was studied in LLC-PK1 cell monolayers grown on a micro-porous membrane filter. The addition of α-human atrial natriuretic peptide (α-hANP) to the apical side of the cells produced a concentration-dependent increase in cyclic GMP (cGMP) formation, the extent of which was similar to that produced by the addition to the basolateral side. More cGMP was formed with α-hANP (1 and 100 nM) than with the C-type natriuretic peptide (100 nM). These findings indicate that the biologically active receptors with high efficacy for α-hANP are expressed in both the apical and basolateral membranes in LLC-PK1 cells.",

keywords = "ANP (atrial natriuretic peptide), Guanylate cyclase, LLC-PK cell, Natriuretic peptide, C-type",

author = "{Yukiya Hashimoto}, Hashimoto and {Junko Ozaki}, Ozaki and {Masato Yasuhara}, Yasuhara and {Ryohei Hori}, Hori and {Shin-ichi Suga}, Suga and {Hiroshi Itoh}, Itoh and {Kazuwa Nakao}, Nakao and {Ken-inchi Inui}, Inui",

note = "Funding Information: This work was supporteidn part by a Grant-in-Aidfo r Scientific Researchfr om the Ministry of EducationS, cience,a nd Culture of Japan.",

year = "1994",

month = aug,

day = "16",

doi = "10.1016/0922-4106(94)90071-X",

language = "英語",

volume = "268",

pages = "443--445",

journal = "European Journal of Pharmacology: Molecular Pharmacology",

issn = "0922-4106",

publisher = "Elsevier B.V.",

number = "3",

}

TY - JOUR

T1 - Functional evidence for an apical ANP receptor in LLC-PK1 kidney epithelial cells

AU - Yukiya Hashimoto, Hashimoto

AU - Junko Ozaki, Ozaki

AU - Masato Yasuhara, Yasuhara

AU - Ryohei Hori, Hori

AU - Shin-ichi Suga, Suga

AU - Hiroshi Itoh, Itoh

AU - Kazuwa Nakao, Nakao

AU - Ken-inchi Inui, Inui

N1 - Funding Information: This work was supporteidn part by a Grant-in-Aidfo r Scientific Researchfr om the Ministry of EducationS, cience,a nd Culture of Japan.

PY - 1994/8/16

Y1 - 1994/8/16

N2 - The localization of natriuretic peptide receptors was studied in LLC-PK1 cell monolayers grown on a micro-porous membrane filter. The addition of α-human atrial natriuretic peptide (α-hANP) to the apical side of the cells produced a concentration-dependent increase in cyclic GMP (cGMP) formation, the extent of which was similar to that produced by the addition to the basolateral side. More cGMP was formed with α-hANP (1 and 100 nM) than with the C-type natriuretic peptide (100 nM). These findings indicate that the biologically active receptors with high efficacy for α-hANP are expressed in both the apical and basolateral membranes in LLC-PK1 cells.

AB - The localization of natriuretic peptide receptors was studied in LLC-PK1 cell monolayers grown on a micro-porous membrane filter. The addition of α-human atrial natriuretic peptide (α-hANP) to the apical side of the cells produced a concentration-dependent increase in cyclic GMP (cGMP) formation, the extent of which was similar to that produced by the addition to the basolateral side. More cGMP was formed with α-hANP (1 and 100 nM) than with the C-type natriuretic peptide (100 nM). These findings indicate that the biologically active receptors with high efficacy for α-hANP are expressed in both the apical and basolateral membranes in LLC-PK1 cells.

KW - ANP (atrial natriuretic peptide)

KW - Guanylate cyclase

KW - LLC-PK cell

KW - Natriuretic peptide, C-type

UR - http://www.scopus.com/inward/record.url?scp=0028169675&partnerID=8YFLogxK

U2 - 10.1016/0922-4106(94)90071-X

DO - 10.1016/0922-4106(94)90071-X

M3 - 学術論文

C2 - 7805770

AN - SCOPUS:0028169675

SN - 0922-4106

VL - 268

SP - 443

EP - 445

JO - European Journal of Pharmacology: Molecular Pharmacology

JF - European Journal of Pharmacology: Molecular Pharmacology

IS - 3

ER -