Fabrication of substrates for multiple cell patterning using a copolymer with a UV-degradable oligoethylene glycol side chain

Hiroaki Ogawa; Yuka Yamazawa; Tadashi Nakaji-Hirabayashi; Hiromi Kitano; Yoshiyuki Saruwatari; Kazuyoshi Matsuoka

doi:10.1039/d2ma00198e

Fabrication of substrates for multiple cell patterning using a copolymer with a UV-degradable oligoethylene glycol side chain

Hiroaki Ogawa, Yuka Yamazawa, Tadashi Nakaji-Hirabayashi^*, Hiromi Kitano, Yoshiyuki Saruwatari, Kazuyoshi Matsuoka

^*Corresponding author for this work

Applied Chemistry

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

A random copolymer of ω-methoxy-(ethylene glycol)₈ acrylamide (MEGAm) and 3-methacryloxypropyltrimethoxysilane (MPTMS) was prepared by free radical polymerization, and glass substrates were modified with the copolymer by silane coupling. MEGAm is a bio-inactive material because of its oligoethylene glycol chain, which can be cleaved by UV irradiation. Therefore, the amount of protein adsorption and cell adhesion to the copolymer-modified glass substrate increased after UV irradiation. Furthermore, when a part of the copolymer-modified glass substrate was subjected to UV irradiation and cells were seeded on the substrate, the cells adhered selectively to the UV-irradiated part. The subsequent UV-irradiation of the other part containing the area where cells adhered caused another kind of cell to adhere to the latter part of the substrate. Thus, we could achieve multiple cell patterning.

Original language	English
Pages (from-to)	5753-5759
Number of pages	7
Journal	Materials Advances
Volume	3
Issue number	14
DOIs	https://doi.org/10.1039/d2ma00198e
State	Published - 2022/05/10

ASJC Scopus subject areas

Chemistry (miscellaneous)
General Materials Science

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10.1039/d2ma00198e

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title = "Fabrication of substrates for multiple cell patterning using a copolymer with a UV-degradable oligoethylene glycol side chain",

abstract = "A random copolymer of ω-methoxy-(ethylene glycol)8 acrylamide (MEGAm) and 3-methacryloxypropyltrimethoxysilane (MPTMS) was prepared by free radical polymerization, and glass substrates were modified with the copolymer by silane coupling. MEGAm is a bio-inactive material because of its oligoethylene glycol chain, which can be cleaved by UV irradiation. Therefore, the amount of protein adsorption and cell adhesion to the copolymer-modified glass substrate increased after UV irradiation. Furthermore, when a part of the copolymer-modified glass substrate was subjected to UV irradiation and cells were seeded on the substrate, the cells adhered selectively to the UV-irradiated part. The subsequent UV-irradiation of the other part containing the area where cells adhered caused another kind of cell to adhere to the latter part of the substrate. Thus, we could achieve multiple cell patterning.",

author = "Hiroaki Ogawa and Yuka Yamazawa and Tadashi Nakaji-Hirabayashi and Hiromi Kitano and Yoshiyuki Saruwatari and Kazuyoshi Matsuoka",

note = "Publisher Copyright: {\textcopyright} 2022 RSC.",

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doi = "10.1039/d2ma00198e",

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T1 - Fabrication of substrates for multiple cell patterning using a copolymer with a UV-degradable oligoethylene glycol side chain

AU - Ogawa, Hiroaki

AU - Yamazawa, Yuka

AU - Nakaji-Hirabayashi, Tadashi

AU - Kitano, Hiromi

AU - Saruwatari, Yoshiyuki

AU - Matsuoka, Kazuyoshi

PY - 2022/5/10

Y1 - 2022/5/10

N2 - A random copolymer of ω-methoxy-(ethylene glycol)8 acrylamide (MEGAm) and 3-methacryloxypropyltrimethoxysilane (MPTMS) was prepared by free radical polymerization, and glass substrates were modified with the copolymer by silane coupling. MEGAm is a bio-inactive material because of its oligoethylene glycol chain, which can be cleaved by UV irradiation. Therefore, the amount of protein adsorption and cell adhesion to the copolymer-modified glass substrate increased after UV irradiation. Furthermore, when a part of the copolymer-modified glass substrate was subjected to UV irradiation and cells were seeded on the substrate, the cells adhered selectively to the UV-irradiated part. The subsequent UV-irradiation of the other part containing the area where cells adhered caused another kind of cell to adhere to the latter part of the substrate. Thus, we could achieve multiple cell patterning.

AB - A random copolymer of ω-methoxy-(ethylene glycol)8 acrylamide (MEGAm) and 3-methacryloxypropyltrimethoxysilane (MPTMS) was prepared by free radical polymerization, and glass substrates were modified with the copolymer by silane coupling. MEGAm is a bio-inactive material because of its oligoethylene glycol chain, which can be cleaved by UV irradiation. Therefore, the amount of protein adsorption and cell adhesion to the copolymer-modified glass substrate increased after UV irradiation. Furthermore, when a part of the copolymer-modified glass substrate was subjected to UV irradiation and cells were seeded on the substrate, the cells adhered selectively to the UV-irradiated part. The subsequent UV-irradiation of the other part containing the area where cells adhered caused another kind of cell to adhere to the latter part of the substrate. Thus, we could achieve multiple cell patterning.

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U2 - 10.1039/d2ma00198e

DO - 10.1039/d2ma00198e

M3 - 学術論文

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SN - 2633-5409

VL - 3

SP - 5753

EP - 5759

JO - Materials Advances

JF - Materials Advances

IS - 14

ER -

Fabrication of substrates for multiple cell patterning using a copolymer with a UV-degradable oligoethylene glycol side chain

Abstract

ASJC Scopus subject areas

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