TY - JOUR
T1 - Expression of tumor necrosis factor α (TNFα) following transient cerebral ischemia
AU - Shioda, S.
AU - Yin, L.
AU - Ohtaki, H.
AU - Nakamachi, T.
AU - Dohi, K.
AU - Iwai, Y.
AU - Funahashi, H.
AU - Makino, R.
PY - 2003
Y1 - 2003
N2 - It has been considered that tumor necrosis factor a (TNFα) is participated in the Alzheimer's, and Parkinson's diseases, brain injury and brain ischemia. However, expression of TNFα after brain ischemia has not been demonstrated in detail. Therefore we examined the cellular expression of TNFα during and after transient middle cerebral artery occlusion (tMCAO) in mice by use of reverse transcriptase-polymerase chain reaction and immunohistochemical technique. TNFα mRNA expression was gradually increased in the neocortex of the ipsilateral hemisphere during ischemia and peaked at 1 hour after reperfusion. Then, the mRNA expression decreased and peaked again at 24 hours after reperfusion. TNFα-like im- munorcactivities were observed in the process such as dendrite of neuron slightly before ischemia, and markedly increased in neurons in addition to the process of the ipsilateral hemisphere at 1 and 24 hours after ischemia. The results suggest that the expression of TNFα is up-regulated in the neurons after tMCAO. TNFα may induce ischemic neuronal cell death during ischemic insult.
AB - It has been considered that tumor necrosis factor a (TNFα) is participated in the Alzheimer's, and Parkinson's diseases, brain injury and brain ischemia. However, expression of TNFα after brain ischemia has not been demonstrated in detail. Therefore we examined the cellular expression of TNFα during and after transient middle cerebral artery occlusion (tMCAO) in mice by use of reverse transcriptase-polymerase chain reaction and immunohistochemical technique. TNFα mRNA expression was gradually increased in the neocortex of the ipsilateral hemisphere during ischemia and peaked at 1 hour after reperfusion. Then, the mRNA expression decreased and peaked again at 24 hours after reperfusion. TNFα-like im- munorcactivities were observed in the process such as dendrite of neuron slightly before ischemia, and markedly increased in neurons in addition to the process of the ipsilateral hemisphere at 1 and 24 hours after ischemia. The results suggest that the expression of TNFα is up-regulated in the neurons after tMCAO. TNFα may induce ischemic neuronal cell death during ischemic insult.
KW - Immunohistochemistry
KW - Mice
KW - Middle cerebral artery occlusion
KW - Tumor necrosis factor a (TNFα)
UR - http://www.scopus.com/inward/record.url?scp=1442303913&partnerID=8YFLogxK
U2 - 10.1007/978-3-7091-0651-8_21
DO - 10.1007/978-3-7091-0651-8_21
M3 - 学術論文
C2 - 14753413
AN - SCOPUS:1442303913
SN - 0065-1419
SP - 93
EP - 96
JO - Acta Neurochirurgica, Supplement
JF - Acta Neurochirurgica, Supplement
IS - 86
ER -