Evaluation of interbilayer and transbilayer transfer dynamics of phospholipids using time-resolved small-angle neutron scattering

Minoru Nakano*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

5 Scopus citations

Abstract

The bilayer structure of biomembranes consists of thousands of lipids, the composition of which is different for each organelle. Since most lipids are synthesized in the endoplasmic reticulum, subsequent distribution to each organelle determines the composition and function of the biomembranes. Thus, interbilayer transfer and transbilayer movement (flip-flop) of phospholipids play important roles in maintaining homeostasis. A crucial task in biophysics and cell biology is to understand how rapidly lipids migrate between bilayers spontaneously or through proteins and to control these lipid dynamics. Time-resolved small-angle neutron scattering (TR-SANS) is a powerful technique to determine the intervesicular exchange and flip-flop rates of lipids in situ and real time. In this review, I explain how TR-SANS detects the interbilayer and transbilayer transfer of phospholipids and introduce recent progress of my group on the evaluation of spontaneous and protein- (or peptide-)mediated lipid transfer in several phospholipid dispersion systems.

Original languageEnglish
Pages (from-to)316-320
Number of pages5
JournalChemical and Pharmaceutical Bulletin
Volume67
Issue number4
DOIs
StatePublished - 2019

Keywords

  • Flip-flop
  • Lipid transfer
  • Neutron scattering
  • Phospholipid

ASJC Scopus subject areas

  • General Chemistry
  • Drug Discovery

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