Evaluation of fractions and isolated polysaccharides from Sasa veitchii for their preventive effects on influenza A virus infection

Kyoko Hayashi; Jung Bum Lee; Yukiyo Kurosaki; Mari Nozawa; Satomi Asai; Kazuo Takeshita; Toshimitsu Hayashi

doi:10.1016/j.jff.2014.05.016

Evaluation of fractions and isolated polysaccharides from Sasa veitchii for their preventive effects on influenza A virus infection

Kyoko Hayashi, Jung Bum Lee, Yukiyo Kurosaki, Mari Nozawa, Satomi Asai, Kazuo Takeshita, Toshimitsu Hayashi^*

^*Corresponding author for this work

Plant Resource Sciences

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

The fractions and polysaccharides from Sasa veitchii were evaluated for their preventive effects on influenza A virus (IFV-A) infection. In IFV-A infected mice, the high-molecular portion (SH) of hot-water extract from S.veitchii significantly reduced virus yields in lung and bronchoalveolar lavage fluid (BALF) and increased in IFV-A-specific secretory IgA in BALF and intestine samples, and neutralizing antibody titers in BALFs and sera. Separation of SH by combination with ion-exchange column and gel filtration yielded six purified polysaccharides. Among them, SH2A1B1 with molecular weight of 29.7kDa was found to reduce virus yields in lung as well as BALF in IFV-A-infected mice. A significant increase in the production of neutralizing antibody was also observed in BALFs on day 15 post-infection. Furthermore, significant enhancement of nitrite oxide production in RAW 264.7 cells was observed. These results suggest that the fractions from S.veitchii and SH2A1B1 could provide useful materials for prevention of IFV-A infection.

Original language	English
Pages (from-to)	25-34
Number of pages	10
Journal	Journal of Functional Foods
Volume	10
DOIs	https://doi.org/10.1016/j.jff.2014.05.016
State	Published - 2014/09/01

Keywords

Influenza A virus
NO production
Neutralizing antibody
Polysaccharide
Sasa veitchii

ASJC Scopus subject areas

Food Science
Medicine (miscellaneous)
Nutrition and Dietetics

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10.1016/j.jff.2014.05.016

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title = "Evaluation of fractions and isolated polysaccharides from Sasa veitchii for their preventive effects on influenza A virus infection",

abstract = "The fractions and polysaccharides from Sasa veitchii were evaluated for their preventive effects on influenza A virus (IFV-A) infection. In IFV-A infected mice, the high-molecular portion (SH) of hot-water extract from S.veitchii significantly reduced virus yields in lung and bronchoalveolar lavage fluid (BALF) and increased in IFV-A-specific secretory IgA in BALF and intestine samples, and neutralizing antibody titers in BALFs and sera. Separation of SH by combination with ion-exchange column and gel filtration yielded six purified polysaccharides. Among them, SH2A1B1 with molecular weight of 29.7kDa was found to reduce virus yields in lung as well as BALF in IFV-A-infected mice. A significant increase in the production of neutralizing antibody was also observed in BALFs on day 15 post-infection. Furthermore, significant enhancement of nitrite oxide production in RAW 264.7 cells was observed. These results suggest that the fractions from S.veitchii and SH2A1B1 could provide useful materials for prevention of IFV-A infection.",

keywords = "Influenza A virus, NO production, Neutralizing antibody, Polysaccharide, Sasa veitchii",

author = "Kyoko Hayashi and Lee, {Jung Bum} and Yukiyo Kurosaki and Mari Nozawa and Satomi Asai and Kazuo Takeshita and Toshimitsu Hayashi",

note = "Publisher Copyright: {\textcopyright} 2014 Elsevier Ltd.",

year = "2014",

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day = "1",

doi = "10.1016/j.jff.2014.05.016",

language = "英語",

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AU - Hayashi, Kyoko

AU - Lee, Jung Bum

AU - Kurosaki, Yukiyo

AU - Nozawa, Mari

AU - Asai, Satomi

AU - Takeshita, Kazuo

AU - Hayashi, Toshimitsu

PY - 2014/9/1

Y1 - 2014/9/1

N2 - The fractions and polysaccharides from Sasa veitchii were evaluated for their preventive effects on influenza A virus (IFV-A) infection. In IFV-A infected mice, the high-molecular portion (SH) of hot-water extract from S.veitchii significantly reduced virus yields in lung and bronchoalveolar lavage fluid (BALF) and increased in IFV-A-specific secretory IgA in BALF and intestine samples, and neutralizing antibody titers in BALFs and sera. Separation of SH by combination with ion-exchange column and gel filtration yielded six purified polysaccharides. Among them, SH2A1B1 with molecular weight of 29.7kDa was found to reduce virus yields in lung as well as BALF in IFV-A-infected mice. A significant increase in the production of neutralizing antibody was also observed in BALFs on day 15 post-infection. Furthermore, significant enhancement of nitrite oxide production in RAW 264.7 cells was observed. These results suggest that the fractions from S.veitchii and SH2A1B1 could provide useful materials for prevention of IFV-A infection.

AB - The fractions and polysaccharides from Sasa veitchii were evaluated for their preventive effects on influenza A virus (IFV-A) infection. In IFV-A infected mice, the high-molecular portion (SH) of hot-water extract from S.veitchii significantly reduced virus yields in lung and bronchoalveolar lavage fluid (BALF) and increased in IFV-A-specific secretory IgA in BALF and intestine samples, and neutralizing antibody titers in BALFs and sera. Separation of SH by combination with ion-exchange column and gel filtration yielded six purified polysaccharides. Among them, SH2A1B1 with molecular weight of 29.7kDa was found to reduce virus yields in lung as well as BALF in IFV-A-infected mice. A significant increase in the production of neutralizing antibody was also observed in BALFs on day 15 post-infection. Furthermore, significant enhancement of nitrite oxide production in RAW 264.7 cells was observed. These results suggest that the fractions from S.veitchii and SH2A1B1 could provide useful materials for prevention of IFV-A infection.

KW - Influenza A virus

KW - NO production

KW - Neutralizing antibody

KW - Polysaccharide

KW - Sasa veitchii

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DO - 10.1016/j.jff.2014.05.016

M3 - 学術論文

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JO - Journal of Functional Foods

JF - Journal of Functional Foods

ER -

Evaluation of fractions and isolated polysaccharides from Sasa veitchii for their preventive effects on influenza A virus infection

Abstract

Keywords

ASJC Scopus subject areas

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