Evaluation of a novel rapid TRC assay for the detection of influenza using nasopharyngeal swabs and gargle samples

Norihito Kaku; Kohji Hashiguchi; Norihiko Akamatsu; Fumiko Wakigawa; Junichi Matsuda; Kenzo Komaru; Takumi Nakao; Yosuke Harada; Atsuko Hara; Naoki Uno; Kei Sakamoto; Yoshitomo Morinaga; Takeshi Kitazaki; Hiroo Hasegawa; Taiga Miyazaki; Masaaki Fukuda; Koichi Izumikawa; Hiroshi Mukae; Katsunori Yanagihara

doi:10.1007/s10096-021-04193-7

Evaluation of a novel rapid TRC assay for the detection of influenza using nasopharyngeal swabs and gargle samples

Norihito Kaku^*, Kohji Hashiguchi, Norihiko Akamatsu, Fumiko Wakigawa, Junichi Matsuda, Kenzo Komaru, Takumi Nakao, Yosuke Harada, Atsuko Hara, Naoki Uno, Kei Sakamoto, Yoshitomo Morinaga, Takeshi Kitazaki, Hiroo Hasegawa, Taiga Miyazaki, Masaaki Fukuda, Koichi Izumikawa, Hiroshi Mukae, Katsunori Yanagihara

^*Corresponding author for this work

Microbiology

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

We evaluated a novel transcription-reverse transcription concerted reaction (TRC) assay that can detect influenza A and B within 15 min using nasopharyngeal swab and gargle samples obtained from patients with influenza-like illness, between January and March 2018 and between January and March 2019. Based on the combined RT-PCR and sequencing results, in the nasal swabs, the sensitivity and specificity of TRC for detecting influenza were calculated as 1.000 and 1.000, respectively. In the gargle samples, the sensitivity and specificity of TRC were 0.946 and 1.000, respectively. The TRC assay showed comparable performance to RT-PCR in the detection of influenza viruses.

Original language	English
Pages (from-to)	1743-1748
Number of pages	6
Journal	European Journal of Clinical Microbiology and Infectious Diseases
Volume	40
Issue number	8
DOIs	https://doi.org/10.1007/s10096-021-04193-7
State	Published - 2021/08

Keywords

Influenza
Rapid detection
TRC method; RT-PCR

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases

Access to Document

10.1007/s10096-021-04193-7

Cite this

Kaku, N., Hashiguchi, K., Akamatsu, N., Wakigawa, F., Matsuda, J., Komaru, K., Nakao, T., Harada, Y., Hara, A., Uno, N., Sakamoto, K., Morinaga, Y., Kitazaki, T., Hasegawa, H., Miyazaki, T., Fukuda, M., Izumikawa, K., Mukae, H., & Yanagihara, K. (2021). Evaluation of a novel rapid TRC assay for the detection of influenza using nasopharyngeal swabs and gargle samples. European Journal of Clinical Microbiology and Infectious Diseases, 40(8), 1743-1748. https://doi.org/10.1007/s10096-021-04193-7

@article{704436919fa54d6dadb880bd7448886e,

title = "Evaluation of a novel rapid TRC assay for the detection of influenza using nasopharyngeal swabs and gargle samples",

abstract = "We evaluated a novel transcription-reverse transcription concerted reaction (TRC) assay that can detect influenza A and B within 15 min using nasopharyngeal swab and gargle samples obtained from patients with influenza-like illness, between January and March 2018 and between January and March 2019. Based on the combined RT-PCR and sequencing results, in the nasal swabs, the sensitivity and specificity of TRC for detecting influenza were calculated as 1.000 and 1.000, respectively. In the gargle samples, the sensitivity and specificity of TRC were 0.946 and 1.000, respectively. The TRC assay showed comparable performance to RT-PCR in the detection of influenza viruses.",

keywords = "Influenza, Rapid detection, TRC method; RT-PCR",

author = "Norihito Kaku and Kohji Hashiguchi and Norihiko Akamatsu and Fumiko Wakigawa and Junichi Matsuda and Kenzo Komaru and Takumi Nakao and Yosuke Harada and Atsuko Hara and Naoki Uno and Kei Sakamoto and Yoshitomo Morinaga and Takeshi Kitazaki and Hiroo Hasegawa and Taiga Miyazaki and Masaaki Fukuda and Koichi Izumikawa and Hiroshi Mukae and Katsunori Yanagihara",

note = "Publisher Copyright: {\textcopyright} 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.",

year = "2021",

month = aug,

doi = "10.1007/s10096-021-04193-7",

language = "英語",

volume = "40",

pages = "1743--1748",

journal = "European Journal of Clinical Microbiology and Infectious Diseases",

issn = "0934-9723",

publisher = "Springer Verlag",

number = "8",

}

Kaku, N, Hashiguchi, K, Akamatsu, N, Wakigawa, F, Matsuda, J, Komaru, K, Nakao, T, Harada, Y, Hara, A, Uno, N, Sakamoto, K, Morinaga, Y, Kitazaki, T, Hasegawa, H, Miyazaki, T, Fukuda, M, Izumikawa, K, Mukae, H & Yanagihara, K 2021, 'Evaluation of a novel rapid TRC assay for the detection of influenza using nasopharyngeal swabs and gargle samples', European Journal of Clinical Microbiology and Infectious Diseases, vol. 40, no. 8, pp. 1743-1748. https://doi.org/10.1007/s10096-021-04193-7

TY - JOUR

T1 - Evaluation of a novel rapid TRC assay for the detection of influenza using nasopharyngeal swabs and gargle samples

AU - Kaku, Norihito

AU - Hashiguchi, Kohji

AU - Akamatsu, Norihiko

AU - Wakigawa, Fumiko

AU - Matsuda, Junichi

AU - Komaru, Kenzo

AU - Nakao, Takumi

AU - Harada, Yosuke

AU - Hara, Atsuko

AU - Uno, Naoki

AU - Sakamoto, Kei

AU - Morinaga, Yoshitomo

AU - Kitazaki, Takeshi

AU - Hasegawa, Hiroo

AU - Miyazaki, Taiga

AU - Fukuda, Masaaki

AU - Izumikawa, Koichi

AU - Mukae, Hiroshi

AU - Yanagihara, Katsunori

PY - 2021/8

Y1 - 2021/8

N2 - We evaluated a novel transcription-reverse transcription concerted reaction (TRC) assay that can detect influenza A and B within 15 min using nasopharyngeal swab and gargle samples obtained from patients with influenza-like illness, between January and March 2018 and between January and March 2019. Based on the combined RT-PCR and sequencing results, in the nasal swabs, the sensitivity and specificity of TRC for detecting influenza were calculated as 1.000 and 1.000, respectively. In the gargle samples, the sensitivity and specificity of TRC were 0.946 and 1.000, respectively. The TRC assay showed comparable performance to RT-PCR in the detection of influenza viruses.

AB - We evaluated a novel transcription-reverse transcription concerted reaction (TRC) assay that can detect influenza A and B within 15 min using nasopharyngeal swab and gargle samples obtained from patients with influenza-like illness, between January and March 2018 and between January and March 2019. Based on the combined RT-PCR and sequencing results, in the nasal swabs, the sensitivity and specificity of TRC for detecting influenza were calculated as 1.000 and 1.000, respectively. In the gargle samples, the sensitivity and specificity of TRC were 0.946 and 1.000, respectively. The TRC assay showed comparable performance to RT-PCR in the detection of influenza viruses.

KW - Influenza

KW - Rapid detection

KW - TRC method; RT-PCR

UR - http://www.scopus.com/inward/record.url?scp=85100904120&partnerID=8YFLogxK

U2 - 10.1007/s10096-021-04193-7

DO - 10.1007/s10096-021-04193-7

M3 - 学術論文

C2 - 33594599

AN - SCOPUS:85100904120

SN - 0934-9723

VL - 40

SP - 1743

EP - 1748

JO - European Journal of Clinical Microbiology and Infectious Diseases

JF - European Journal of Clinical Microbiology and Infectious Diseases

IS - 8

ER -

Evaluation of a novel rapid TRC assay for the detection of influenza using nasopharyngeal swabs and gargle samples

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Fingerprint

Cite this