Ether extract of fetal calf serum protects cultured rat cortical neurons against glutamate cytotoxicity

Toshiaki Kume; Hanae Kohchiyama; Hiroyuki Nishikawa; Takehiko Maeda; Shuji Kaneko; Akinori Akaike; Naoki Noda; Tetsuro Fujita

doi:10.1254/jjp.73.371

Ether extract of fetal calf serum protects cultured rat cortical neurons against glutamate cytotoxicity

Toshiaki Kume, Hanae Kohchiyama, Hiroyuki Nishikawa, Takehiko Maeda, Shuji Kaneko, Akinori Akaike^*, Naoki Noda, Tetsuro Fujita

^*Corresponding author for this work

Applied Pharmacology

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

The effects of an ether extract of fetal calf serum (EE-FCS) on glutamate-induced cytotoxicity were examined using primary cultures of rat cortical neurons. The simultaneous addition of EE-FCS and glutamate reduced glutamate cytotoxicity in a concentration-dependent manner. EE-FCS also reduced the cytotoxicity induced by S-nitrosocysteine, a nitric oxide (NO) donor. The findings indicate that EE-FCS contains lipid-soluble, non-peptide substances with neuroprotective actions against NO-mediated glutamate neurotoxicity.

Original language	English
Pages (from-to)	371-374
Number of pages	4
Journal	Japanese Journal of Pharmacology
Volume	73
Issue number	4
DOIs	https://doi.org/10.1254/jjp.73.371
State	Published - 1997/04

Keywords

Fetal calf serum
Glutamate
Nitric oxide

ASJC Scopus subject areas

Pharmacology

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10.1254/jjp.73.371

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title = "Ether extract of fetal calf serum protects cultured rat cortical neurons against glutamate cytotoxicity",

abstract = "The effects of an ether extract of fetal calf serum (EE-FCS) on glutamate-induced cytotoxicity were examined using primary cultures of rat cortical neurons. The simultaneous addition of EE-FCS and glutamate reduced glutamate cytotoxicity in a concentration-dependent manner. EE-FCS also reduced the cytotoxicity induced by S-nitrosocysteine, a nitric oxide (NO) donor. The findings indicate that EE-FCS contains lipid-soluble, non-peptide substances with neuroprotective actions against NO-mediated glutamate neurotoxicity.",

keywords = "Fetal calf serum, Glutamate, Nitric oxide",

author = "Toshiaki Kume and Hanae Kohchiyama and Hiroyuki Nishikawa and Takehiko Maeda and Shuji Kaneko and Akinori Akaike and Naoki Noda and Tetsuro Fujita",

year = "1997",

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doi = "10.1254/jjp.73.371",

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T1 - Ether extract of fetal calf serum protects cultured rat cortical neurons against glutamate cytotoxicity

AU - Kume, Toshiaki

AU - Kohchiyama, Hanae

AU - Nishikawa, Hiroyuki

AU - Maeda, Takehiko

AU - Kaneko, Shuji

AU - Akaike, Akinori

AU - Noda, Naoki

AU - Fujita, Tetsuro

PY - 1997/4

Y1 - 1997/4

N2 - The effects of an ether extract of fetal calf serum (EE-FCS) on glutamate-induced cytotoxicity were examined using primary cultures of rat cortical neurons. The simultaneous addition of EE-FCS and glutamate reduced glutamate cytotoxicity in a concentration-dependent manner. EE-FCS also reduced the cytotoxicity induced by S-nitrosocysteine, a nitric oxide (NO) donor. The findings indicate that EE-FCS contains lipid-soluble, non-peptide substances with neuroprotective actions against NO-mediated glutamate neurotoxicity.

AB - The effects of an ether extract of fetal calf serum (EE-FCS) on glutamate-induced cytotoxicity were examined using primary cultures of rat cortical neurons. The simultaneous addition of EE-FCS and glutamate reduced glutamate cytotoxicity in a concentration-dependent manner. EE-FCS also reduced the cytotoxicity induced by S-nitrosocysteine, a nitric oxide (NO) donor. The findings indicate that EE-FCS contains lipid-soluble, non-peptide substances with neuroprotective actions against NO-mediated glutamate neurotoxicity.

KW - Fetal calf serum

KW - Glutamate

KW - Nitric oxide

UR - http://www.scopus.com/inward/record.url?scp=0030919973&partnerID=8YFLogxK

U2 - 10.1254/jjp.73.371

DO - 10.1254/jjp.73.371

M3 - 学術論文

C2 - 9165377

AN - SCOPUS:0030919973

SN - 0021-5198

VL - 73

SP - 371

EP - 374

JO - Japanese Journal of Pharmacology

JF - Japanese Journal of Pharmacology

IS - 4

ER -

Ether extract of fetal calf serum protects cultured rat cortical neurons against glutamate cytotoxicity

Abstract

Keywords

ASJC Scopus subject areas

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