Establishment and characterization of a novel xenograft model of human gastrointestinal stromal tumor in mice

Makoto Moriyama; Yutaka Shimada; Takuya Nagata; Tetsuya Omura; Shinichi Sekine; Koshi Matsui; Isaku Yoshioka; Tomoyuki Okumura; Shigeaki Sawada; Toru Yoshida; Kazuhiro Tsukada

Establishment and characterization of a novel xenograft model of human gastrointestinal stromal tumor in mice

Makoto Moriyama^*, Yutaka Shimada, Takuya Nagata, Tetsuya Omura, Shinichi Sekine, Koshi Matsui, Isaku Yoshioka, Tomoyuki Okumura, Shigeaki Sawada, Toru Yoshida, Kazuhiro Tsukada

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Background: The clinical outcome of gastrointestinal stromal tumor (GIST) has been improved by the introduction of molecular-targeting drugs. However, resistance to these drugs appears during the course of treatment. The aim of this study was to establish and characterize a human xenograft model of GIST. Materials and Methods: GIST tissue from a patient with esophageal GIST was implanted under the skin of a NOD-SCID mouse. The tumor became successfully engrafted and we investigated the effects of imatinib and sunitinib on this model. KIT mutation was investigated by complementary DNA analysis, and c-KIT (CD117) expression was evaluated by immunohistological staining. Results: cDNA analysis of the tumor revealed a KIT mutation in exon 11. c-KIT expression was observed in each passaged tumor. Both imatinib and sunitinib significantly reduced the size of the xenograft tumor. Conclusion: We established a novel xenograft model of human GIST in mice. This xenograft model may be useful for studying GIST.

Original language	English
Pages (from-to)	175-182
Number of pages	8
Journal	Anticancer Research
Volume	33
Issue number	1
State	Published - 2013/01

Keywords

Gastrointestinal stromal tumor
Imatinib
Mouse
Sunitinib
Xenograft

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

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title = "Establishment and characterization of a novel xenograft model of human gastrointestinal stromal tumor in mice",

abstract = "Background: The clinical outcome of gastrointestinal stromal tumor (GIST) has been improved by the introduction of molecular-targeting drugs. However, resistance to these drugs appears during the course of treatment. The aim of this study was to establish and characterize a human xenograft model of GIST. Materials and Methods: GIST tissue from a patient with esophageal GIST was implanted under the skin of a NOD-SCID mouse. The tumor became successfully engrafted and we investigated the effects of imatinib and sunitinib on this model. KIT mutation was investigated by complementary DNA analysis, and c-KIT (CD117) expression was evaluated by immunohistological staining. Results: cDNA analysis of the tumor revealed a KIT mutation in exon 11. c-KIT expression was observed in each passaged tumor. Both imatinib and sunitinib significantly reduced the size of the xenograft tumor. Conclusion: We established a novel xenograft model of human GIST in mice. This xenograft model may be useful for studying GIST.",

keywords = "Gastrointestinal stromal tumor, Imatinib, Mouse, Sunitinib, Xenograft",

author = "Makoto Moriyama and Yutaka Shimada and Takuya Nagata and Tetsuya Omura and Shinichi Sekine and Koshi Matsui and Isaku Yoshioka and Tomoyuki Okumura and Shigeaki Sawada and Toru Yoshida and Kazuhiro Tsukada",

year = "2013",

month = jan,

language = "英語",

volume = "33",

pages = "175--182",

journal = "Anticancer Research",

issn = "0250-7005",

publisher = "International Institute of Anticancer Research",

number = "1",

}

TY - JOUR

T1 - Establishment and characterization of a novel xenograft model of human gastrointestinal stromal tumor in mice

AU - Moriyama, Makoto

AU - Shimada, Yutaka

AU - Nagata, Takuya

AU - Omura, Tetsuya

AU - Sekine, Shinichi

AU - Matsui, Koshi

AU - Yoshioka, Isaku

AU - Okumura, Tomoyuki

AU - Sawada, Shigeaki

AU - Yoshida, Toru

AU - Tsukada, Kazuhiro

PY - 2013/1

Y1 - 2013/1

N2 - Background: The clinical outcome of gastrointestinal stromal tumor (GIST) has been improved by the introduction of molecular-targeting drugs. However, resistance to these drugs appears during the course of treatment. The aim of this study was to establish and characterize a human xenograft model of GIST. Materials and Methods: GIST tissue from a patient with esophageal GIST was implanted under the skin of a NOD-SCID mouse. The tumor became successfully engrafted and we investigated the effects of imatinib and sunitinib on this model. KIT mutation was investigated by complementary DNA analysis, and c-KIT (CD117) expression was evaluated by immunohistological staining. Results: cDNA analysis of the tumor revealed a KIT mutation in exon 11. c-KIT expression was observed in each passaged tumor. Both imatinib and sunitinib significantly reduced the size of the xenograft tumor. Conclusion: We established a novel xenograft model of human GIST in mice. This xenograft model may be useful for studying GIST.

AB - Background: The clinical outcome of gastrointestinal stromal tumor (GIST) has been improved by the introduction of molecular-targeting drugs. However, resistance to these drugs appears during the course of treatment. The aim of this study was to establish and characterize a human xenograft model of GIST. Materials and Methods: GIST tissue from a patient with esophageal GIST was implanted under the skin of a NOD-SCID mouse. The tumor became successfully engrafted and we investigated the effects of imatinib and sunitinib on this model. KIT mutation was investigated by complementary DNA analysis, and c-KIT (CD117) expression was evaluated by immunohistological staining. Results: cDNA analysis of the tumor revealed a KIT mutation in exon 11. c-KIT expression was observed in each passaged tumor. Both imatinib and sunitinib significantly reduced the size of the xenograft tumor. Conclusion: We established a novel xenograft model of human GIST in mice. This xenograft model may be useful for studying GIST.

KW - Gastrointestinal stromal tumor

KW - Imatinib

KW - Mouse

KW - Sunitinib

KW - Xenograft

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M3 - 学術論文

C2 - 23267143

AN - SCOPUS:84873026796

SN - 0250-7005

VL - 33

SP - 175

EP - 182

JO - Anticancer Research

JF - Anticancer Research

IS - 1

ER -

Establishment and characterization of a novel xenograft model of human gastrointestinal stromal tumor in mice

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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