Epithelial to mesenchymal transition might be induced via CD44 isoform switching in colorectal cancer

Naoki Mashita; Suguru Yamada; Goro Nakayama; Chie Tanaka; Naoki Iwata; Mitsuro Kanda; Daisuke Kobayashi; Tsutomu Fujii; Hiroyuki Sugimoto; Masahiko Koike; Shuji Nomoto; Michitaka Fujiwara; Yasuhiro Kodera

doi:10.1002/jso.23705

Epithelial to mesenchymal transition might be induced via CD44 isoform switching in colorectal cancer

Naoki Mashita, Suguru Yamada^*, Goro Nakayama, Chie Tanaka, Naoki Iwata, Mitsuro Kanda, Daisuke Kobayashi, Tsutomu Fujii, Hiroyuki Sugimoto, Masahiko Koike, Shuji Nomoto, Michitaka Fujiwara, Yasuhiro Kodera

^*Corresponding author for this work

Department of Surgery & Science

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

Background: Isoform switching of CD44 is associated with epithelial to mesenchymal transition (EMT) in several cancers; however, the clinical implications of this remain unclear for colorectal cancer (CRC).

Methods: We measured expression levels of E-cadherin, vimentin, CD44 standard (CD44s) and CD44 variant 9 (CD44v9) transcripts in 14 CRC cell lines and 150 CRC patients. We determined EMT and CD44 status by calculating vimentin/E-cadherin and CD44s/CD44v9 expression ratios, respectively. Associations between EMT status and CD44 isoform switching, and between clinicopathological factors and prognosis were analyzed.

Results: CD44s was highly expressed in mesenchymal-type cell lines, while CD44v9 was highly expressed in epithelial-type cell lines. CD44 knockdown resulted in decreased levels of vimentin expression, and significantly reduced proliferation, migration and invasion of cells. In CRC patients, the mesenchymal group and the high CD44 status group exhibited significantly poorer survival than that for the epithelial group (5-year survival; 62.1% vs. 85.5%, P=0.0085) and the low CD44 status group (5-year survival; 66.1% vs. 85.0%, P=0.0251). On multivariate analysis, CD44 status was an independent prognostic factor.

Conclusions: The status of EMT and CD44 is a critical prognostic factor, with CD44 isoform switching a possible trigger for EMT in CRC.

Original language	English
Pages (from-to)	745-751
Number of pages	7
Journal	Journal of Surgical Oncology
Volume	110
Issue number	6
DOIs	https://doi.org/10.1002/jso.23705
State	Published - 2014/11/01

Keywords

CD44
Colorectal cancer
Epithelial to mesenchymal transition

ASJC Scopus subject areas

Surgery
Oncology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/jso.23705

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@article{7c67749d266c4d1a90d8d2c9be92cf65,

title = "Epithelial to mesenchymal transition might be induced via CD44 isoform switching in colorectal cancer",

abstract = "Background: Isoform switching of CD44 is associated with epithelial to mesenchymal transition (EMT) in several cancers; however, the clinical implications of this remain unclear for colorectal cancer (CRC).Methods: We measured expression levels of E-cadherin, vimentin, CD44 standard (CD44s) and CD44 variant 9 (CD44v9) transcripts in 14 CRC cell lines and 150 CRC patients. We determined EMT and CD44 status by calculating vimentin/E-cadherin and CD44s/CD44v9 expression ratios, respectively. Associations between EMT status and CD44 isoform switching, and between clinicopathological factors and prognosis were analyzed.Results: CD44s was highly expressed in mesenchymal-type cell lines, while CD44v9 was highly expressed in epithelial-type cell lines. CD44 knockdown resulted in decreased levels of vimentin expression, and significantly reduced proliferation, migration and invasion of cells. In CRC patients, the mesenchymal group and the high CD44 status group exhibited significantly poorer survival than that for the epithelial group (5-year survival; 62.1% vs. 85.5%, P=0.0085) and the low CD44 status group (5-year survival; 66.1% vs. 85.0%, P=0.0251). On multivariate analysis, CD44 status was an independent prognostic factor.Conclusions: The status of EMT and CD44 is a critical prognostic factor, with CD44 isoform switching a possible trigger for EMT in CRC.",

keywords = "CD44, Colorectal cancer, Epithelial to mesenchymal transition",

author = "Naoki Mashita and Suguru Yamada and Goro Nakayama and Chie Tanaka and Naoki Iwata and Mitsuro Kanda and Daisuke Kobayashi and Tsutomu Fujii and Hiroyuki Sugimoto and Masahiko Koike and Shuji Nomoto and Michitaka Fujiwara and Yasuhiro Kodera",

note = "Publisher Copyright: {\textcopyright} 2014 Wiley Periodicals, Inc.",

year = "2014",

month = nov,

day = "1",

doi = "10.1002/jso.23705",

language = "英語",

volume = "110",

pages = "745--751",

journal = "Journal of Surgical Oncology",

issn = "0022-4790",

publisher = "Wiley-Liss Inc.",

number = "6",

}

TY - JOUR

T1 - Epithelial to mesenchymal transition might be induced via CD44 isoform switching in colorectal cancer

AU - Mashita, Naoki

AU - Yamada, Suguru

AU - Nakayama, Goro

AU - Tanaka, Chie

AU - Iwata, Naoki

AU - Kanda, Mitsuro

AU - Kobayashi, Daisuke

AU - Fujii, Tsutomu

AU - Sugimoto, Hiroyuki

AU - Koike, Masahiko

AU - Nomoto, Shuji

AU - Fujiwara, Michitaka

AU - Kodera, Yasuhiro

PY - 2014/11/1

Y1 - 2014/11/1

N2 - Background: Isoform switching of CD44 is associated with epithelial to mesenchymal transition (EMT) in several cancers; however, the clinical implications of this remain unclear for colorectal cancer (CRC).Methods: We measured expression levels of E-cadherin, vimentin, CD44 standard (CD44s) and CD44 variant 9 (CD44v9) transcripts in 14 CRC cell lines and 150 CRC patients. We determined EMT and CD44 status by calculating vimentin/E-cadherin and CD44s/CD44v9 expression ratios, respectively. Associations between EMT status and CD44 isoform switching, and between clinicopathological factors and prognosis were analyzed.Results: CD44s was highly expressed in mesenchymal-type cell lines, while CD44v9 was highly expressed in epithelial-type cell lines. CD44 knockdown resulted in decreased levels of vimentin expression, and significantly reduced proliferation, migration and invasion of cells. In CRC patients, the mesenchymal group and the high CD44 status group exhibited significantly poorer survival than that for the epithelial group (5-year survival; 62.1% vs. 85.5%, P=0.0085) and the low CD44 status group (5-year survival; 66.1% vs. 85.0%, P=0.0251). On multivariate analysis, CD44 status was an independent prognostic factor.Conclusions: The status of EMT and CD44 is a critical prognostic factor, with CD44 isoform switching a possible trigger for EMT in CRC.

AB - Background: Isoform switching of CD44 is associated with epithelial to mesenchymal transition (EMT) in several cancers; however, the clinical implications of this remain unclear for colorectal cancer (CRC).Methods: We measured expression levels of E-cadherin, vimentin, CD44 standard (CD44s) and CD44 variant 9 (CD44v9) transcripts in 14 CRC cell lines and 150 CRC patients. We determined EMT and CD44 status by calculating vimentin/E-cadherin and CD44s/CD44v9 expression ratios, respectively. Associations between EMT status and CD44 isoform switching, and between clinicopathological factors and prognosis were analyzed.Results: CD44s was highly expressed in mesenchymal-type cell lines, while CD44v9 was highly expressed in epithelial-type cell lines. CD44 knockdown resulted in decreased levels of vimentin expression, and significantly reduced proliferation, migration and invasion of cells. In CRC patients, the mesenchymal group and the high CD44 status group exhibited significantly poorer survival than that for the epithelial group (5-year survival; 62.1% vs. 85.5%, P=0.0085) and the low CD44 status group (5-year survival; 66.1% vs. 85.0%, P=0.0251). On multivariate analysis, CD44 status was an independent prognostic factor.Conclusions: The status of EMT and CD44 is a critical prognostic factor, with CD44 isoform switching a possible trigger for EMT in CRC.

KW - CD44

KW - Colorectal cancer

KW - Epithelial to mesenchymal transition

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U2 - 10.1002/jso.23705

DO - 10.1002/jso.23705

M3 - 学術論文

C2 - 24975268

AN - SCOPUS:84911998199

SN - 0022-4790

VL - 110

SP - 745

EP - 751

JO - Journal of Surgical Oncology

JF - Journal of Surgical Oncology

IS - 6

ER -

Epithelial to mesenchymal transition might be induced via CD44 isoform switching in colorectal cancer

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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