(-)-Epigallocatechin gallate inhibits prostaglandin D2-stimulated HSP27 induction via suppression of the p44/p42 MAP kinase pathway in osteoblasts

Junichi Yamauchi; Shinji Takai; Rie Matsushima-Nishiwaki; Yoshiteru Hanai; Tomoaki Doi; Hisaaki Kato; Shinji Ogura; Kanefusa Kato; Haruhiko Tokuda; Osamu Kozawa

doi:10.1016/j.plefa.2007.09.001

(-)-Epigallocatechin gallate inhibits prostaglandin D₂-stimulated HSP27 induction via suppression of the p44/p42 MAP kinase pathway in osteoblasts

Junichi Yamauchi, Shinji Takai, Rie Matsushima-Nishiwaki, Yoshiteru Hanai, Tomoaki Doi, Hisaaki Kato, Shinji Ogura, Kanefusa Kato, Haruhiko Tokuda, Osamu Kozawa^*

^*Corresponding author for this work

Emergency Medicine

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

We previously reported that prostaglandin D₂ (PGD₂) stimulates heat shock protein 27 (HSP27) induction through p38 mitogen-activated protein (MAP) kinase, stress-activated protein kinase (SAPK)/c-Jun N-terminal kinase (JNK) and p44/p42 MAP kinase in osteoblast-like MC3T3-E1 cells. In the present study, we investigated whether (-)-epigallocatechin gallate (EGCG), the major polyphenol found in green tea, affects the induction of HSP27 in these cells and the mechanism. EGCG significantly reduced the HSP27 induction stimulated by PGD₂ without affecting the levels of HSP70. The PGD₂-induced phosphorylation of p38 MAP kinase or SAPK/JNK was not affected by EGCG. On the contrary, EGCG markedly suppressed the PGD₂-induced phosphorylation of p44/p42 MAP kinase and MEK1/2. However, the PGD₂-induced phosphorylation of Raf-1 was not inhibited by EGCG. These results strongly suggest that EGCG suppresses the PGD₂-stimulated induction of HSP27 at the point between Raf-1 and MEK1/2 in osteoblasts.

Original language	English
Pages (from-to)	173-179
Number of pages	7
Journal	Prostaglandins Leukotrienes and Essential Fatty Acids
Volume	77
Issue number	3-4
DOIs	https://doi.org/10.1016/j.plefa.2007.09.001
State	Published - 2007/10

ASJC Scopus subject areas

Clinical Biochemistry
Cell Biology

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10.1016/j.plefa.2007.09.001

Cite this

Yamauchi, J., Takai, S., Matsushima-Nishiwaki, R., Hanai, Y., Doi, T., Kato, H., Ogura, S., Kato, K., Tokuda, H., & Kozawa, O. (2007). (-)-Epigallocatechin gallate inhibits prostaglandin D₂-stimulated HSP27 induction via suppression of the p44/p42 MAP kinase pathway in osteoblasts. Prostaglandins Leukotrienes and Essential Fatty Acids, 77(3-4), 173-179. https://doi.org/10.1016/j.plefa.2007.09.001

@article{d3a3eb65878b4332bbf091d10a38c751,

title = "(-)-Epigallocatechin gallate inhibits prostaglandin D2-stimulated HSP27 induction via suppression of the p44/p42 MAP kinase pathway in osteoblasts",

abstract = "We previously reported that prostaglandin D2 (PGD2) stimulates heat shock protein 27 (HSP27) induction through p38 mitogen-activated protein (MAP) kinase, stress-activated protein kinase (SAPK)/c-Jun N-terminal kinase (JNK) and p44/p42 MAP kinase in osteoblast-like MC3T3-E1 cells. In the present study, we investigated whether (-)-epigallocatechin gallate (EGCG), the major polyphenol found in green tea, affects the induction of HSP27 in these cells and the mechanism. EGCG significantly reduced the HSP27 induction stimulated by PGD2 without affecting the levels of HSP70. The PGD2-induced phosphorylation of p38 MAP kinase or SAPK/JNK was not affected by EGCG. On the contrary, EGCG markedly suppressed the PGD2-induced phosphorylation of p44/p42 MAP kinase and MEK1/2. However, the PGD2-induced phosphorylation of Raf-1 was not inhibited by EGCG. These results strongly suggest that EGCG suppresses the PGD2-stimulated induction of HSP27 at the point between Raf-1 and MEK1/2 in osteoblasts.",

author = "Junichi Yamauchi and Shinji Takai and Rie Matsushima-Nishiwaki and Yoshiteru Hanai and Tomoaki Doi and Hisaaki Kato and Shinji Ogura and Kanefusa Kato and Haruhiko Tokuda and Osamu Kozawa",

note = "Funding Information: We are very grateful to Yoko Kawamura for her skillful technical assistance. This work was supported in part by Grant-in-Aid for Scientific Research (16590873 and 16591482) for the Ministry of Education, Science, Sports and Culture of Japan, the Research Grants for Longevity Sciences (15A-1 and 15C-2), Research on Proteomics and Research on Fracture and Dementia from Ministry of Health, Labour and Welfare of Japan.",

year = "2007",

month = oct,

doi = "10.1016/j.plefa.2007.09.001",

language = "英語",

volume = "77",

pages = "173--179",

journal = "Prostaglandins Leukotrienes and Essential Fatty Acids",

issn = "0952-3278",

publisher = "Churchill Livingstone",

number = "3-4",

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Yamauchi, J, Takai, S, Matsushima-Nishiwaki, R, Hanai, Y, Doi, T, Kato, H, Ogura, S, Kato, K, Tokuda, H & Kozawa, O 2007, '(-)-Epigallocatechin gallate inhibits prostaglandin D₂-stimulated HSP27 induction via suppression of the p44/p42 MAP kinase pathway in osteoblasts', Prostaglandins Leukotrienes and Essential Fatty Acids, vol. 77, no. 3-4, pp. 173-179. https://doi.org/10.1016/j.plefa.2007.09.001

(-)-Epigallocatechin gallate inhibits prostaglandin D₂-stimulated HSP27 induction via suppression of the p44/p42 MAP kinase pathway in osteoblasts. / Yamauchi, Junichi; Takai, Shinji; Matsushima-Nishiwaki, Rie et al.
In: Prostaglandins Leukotrienes and Essential Fatty Acids, Vol. 77, No. 3-4, 10.2007, p. 173-179.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - (-)-Epigallocatechin gallate inhibits prostaglandin D2-stimulated HSP27 induction via suppression of the p44/p42 MAP kinase pathway in osteoblasts

AU - Yamauchi, Junichi

AU - Takai, Shinji

AU - Matsushima-Nishiwaki, Rie

AU - Hanai, Yoshiteru

AU - Doi, Tomoaki

AU - Kato, Hisaaki

AU - Ogura, Shinji

AU - Kato, Kanefusa

AU - Tokuda, Haruhiko

AU - Kozawa, Osamu

N1 - Funding Information: We are very grateful to Yoko Kawamura for her skillful technical assistance. This work was supported in part by Grant-in-Aid for Scientific Research (16590873 and 16591482) for the Ministry of Education, Science, Sports and Culture of Japan, the Research Grants for Longevity Sciences (15A-1 and 15C-2), Research on Proteomics and Research on Fracture and Dementia from Ministry of Health, Labour and Welfare of Japan.

PY - 2007/10

Y1 - 2007/10

N2 - We previously reported that prostaglandin D2 (PGD2) stimulates heat shock protein 27 (HSP27) induction through p38 mitogen-activated protein (MAP) kinase, stress-activated protein kinase (SAPK)/c-Jun N-terminal kinase (JNK) and p44/p42 MAP kinase in osteoblast-like MC3T3-E1 cells. In the present study, we investigated whether (-)-epigallocatechin gallate (EGCG), the major polyphenol found in green tea, affects the induction of HSP27 in these cells and the mechanism. EGCG significantly reduced the HSP27 induction stimulated by PGD2 without affecting the levels of HSP70. The PGD2-induced phosphorylation of p38 MAP kinase or SAPK/JNK was not affected by EGCG. On the contrary, EGCG markedly suppressed the PGD2-induced phosphorylation of p44/p42 MAP kinase and MEK1/2. However, the PGD2-induced phosphorylation of Raf-1 was not inhibited by EGCG. These results strongly suggest that EGCG suppresses the PGD2-stimulated induction of HSP27 at the point between Raf-1 and MEK1/2 in osteoblasts.

AB - We previously reported that prostaglandin D2 (PGD2) stimulates heat shock protein 27 (HSP27) induction through p38 mitogen-activated protein (MAP) kinase, stress-activated protein kinase (SAPK)/c-Jun N-terminal kinase (JNK) and p44/p42 MAP kinase in osteoblast-like MC3T3-E1 cells. In the present study, we investigated whether (-)-epigallocatechin gallate (EGCG), the major polyphenol found in green tea, affects the induction of HSP27 in these cells and the mechanism. EGCG significantly reduced the HSP27 induction stimulated by PGD2 without affecting the levels of HSP70. The PGD2-induced phosphorylation of p38 MAP kinase or SAPK/JNK was not affected by EGCG. On the contrary, EGCG markedly suppressed the PGD2-induced phosphorylation of p44/p42 MAP kinase and MEK1/2. However, the PGD2-induced phosphorylation of Raf-1 was not inhibited by EGCG. These results strongly suggest that EGCG suppresses the PGD2-stimulated induction of HSP27 at the point between Raf-1 and MEK1/2 in osteoblasts.

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U2 - 10.1016/j.plefa.2007.09.001

DO - 10.1016/j.plefa.2007.09.001

M3 - 学術論文

C2 - 17949962

AN - SCOPUS:36248999475

SN - 0952-3278

VL - 77

SP - 173

EP - 179

JO - Prostaglandins Leukotrienes and Essential Fatty Acids

JF - Prostaglandins Leukotrienes and Essential Fatty Acids

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