Enzymatic Fabrication of Multifunctional Amyloid-based Protein Assembly

We developed a method to orthogonally immobilize multiple functional molecules on amyloid aggregates using different enzymes (sortase A and transglutaminase). Orthogonal binding was performed via the substrate amino acid sequence of each enzyme, and the amount of molecular immobilization could be changed according to the amount of substrate sequence. This method is expected to have applications such as improving the sensitivity of immunoassays and visualization of amyloid elongation reactions.