Electrical activity controlling system for a mediator-coexisting alcohol dehydrogenase-NAD conductive membrane

Soichi Yabuki*, Hiroaki Shinohara, Yoshihito Ikariyama, Masuo Aizawa

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

85 Scopus citations

Abstract

A mediator-coexisting alcohol dehydrogenase-NAD conductive membrane was prepared by electro-chemical polymerization in a pyrrole solution containing alcohol dehydrogenase, NAD, and Meldola's blue. The polypyrrole membrane has enzyme activity as well as electroconductivity. NADH, produced enzymatically in the presence of ethyl alcohol, was oxidized with the aid of Meldola's blue in the membrane. The current of NADH oxidation was dependent on the alcohol concentration as well as the applied potential, i.e., the enzyme activity of the membrane was controlled electrically by the smooth and efficient electron transferring system, ethanol-enzyme-coenzyme-mediator, prepared in a polypyrrole matrix as a conductive interface.

Original languageEnglish
Pages (from-to)179-187
Number of pages9
JournalJournal of Electroanalytical Chemistry
Volume277
Issue number1-2
DOIs
StatePublished - 1990/01/10

ASJC Scopus subject areas

  • Analytical Chemistry
  • General Chemical Engineering
  • Electrochemistry

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