Abstract
A mediator-coexisting alcohol dehydrogenase-NAD conductive membrane was prepared by electro-chemical polymerization in a pyrrole solution containing alcohol dehydrogenase, NAD, and Meldola's blue. The polypyrrole membrane has enzyme activity as well as electroconductivity. NADH, produced enzymatically in the presence of ethyl alcohol, was oxidized with the aid of Meldola's blue in the membrane. The current of NADH oxidation was dependent on the alcohol concentration as well as the applied potential, i.e., the enzyme activity of the membrane was controlled electrically by the smooth and efficient electron transferring system, ethanol-enzyme-coenzyme-mediator, prepared in a polypyrrole matrix as a conductive interface.
| Original language | English |
|---|---|
| Pages (from-to) | 179-187 |
| Number of pages | 9 |
| Journal | Journal of Electroanalytical Chemistry |
| Volume | 277 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - 1990/01/10 |
ASJC Scopus subject areas
- Analytical Chemistry
- General Chemical Engineering
- Electrochemistry