TY - JOUR
T1 - Efflux of intracellular α-ketoglutarate via p-aminohippurate /dicarboxylate exchange in OK kidney epithelial cells
AU - Nagai, Junya
AU - Yano, Ikuko
AU - Hashimoto, Yukiya
AU - Takano, Mikihisa
AU - Inui, Ken Ichi
PY - 1998
Y1 - 1998
N2 - The involvement of intracellular α-ketoglutarate (α-KG) in p- aminohippurate (PAH) transport was investigated in OK kidney epithelial cells. Efflux of intracellular α-KG from the OK cells to the basolateral side was increased by applying PAH to the basolateral side of the cells. In contrast, the intracellular α-KG concentration was not influenced by the addition of PAH. The α-KG efflux across the basolateral membrane induced by PAH was higher than that across the apical membrane. Probenecid inhibited the PAH-dependent α-KG efflux. The α-KG efflux to the basolateral side was saturable with increasing concentration of PAH in the basolateral medium. Antimycin A, a metabolic inhibitor, inhibited [14C]PAH uptake across the basolateral membrane of OK cells in a dose-dependent manner. In addition, both the α-KG efflux induced by PAH and the intracellular α-KG concentration were decreased, by antimycin A dose-dependently. These results directly show that α-KG generated by intracellular metabolism is effluxed via PAH/dicarboxylate exchange in the basolateral membrane of OK cells.
AB - The involvement of intracellular α-ketoglutarate (α-KG) in p- aminohippurate (PAH) transport was investigated in OK kidney epithelial cells. Efflux of intracellular α-KG from the OK cells to the basolateral side was increased by applying PAH to the basolateral side of the cells. In contrast, the intracellular α-KG concentration was not influenced by the addition of PAH. The α-KG efflux across the basolateral membrane induced by PAH was higher than that across the apical membrane. Probenecid inhibited the PAH-dependent α-KG efflux. The α-KG efflux to the basolateral side was saturable with increasing concentration of PAH in the basolateral medium. Antimycin A, a metabolic inhibitor, inhibited [14C]PAH uptake across the basolateral membrane of OK cells in a dose-dependent manner. In addition, both the α-KG efflux induced by PAH and the intracellular α-KG concentration were decreased, by antimycin A dose-dependently. These results directly show that α-KG generated by intracellular metabolism is effluxed via PAH/dicarboxylate exchange in the basolateral membrane of OK cells.
UR - http://www.scopus.com/inward/record.url?scp=0031863860&partnerID=8YFLogxK
U2 - 10.1016/s0022-3565(24)37480-4
DO - 10.1016/s0022-3565(24)37480-4
M3 - 学術論文
C2 - 9580579
AN - SCOPUS:0031863860
SN - 0022-3565
VL - 285
SP - 422
EP - 427
JO - Journal of Pharmacology and Experimental Therapeutics
JF - Journal of Pharmacology and Experimental Therapeutics
IS - 2
ER -