TY - JOUR
T1 - Effects of low-intensity pulsed ultrasound on osteoclasts
T2 - Analysis with goldfish scales as a model of bone
AU - Hanmoto, Taizo
AU - Tabuchi, Yoshiaki
AU - Ikegame, Mika
AU - Kondo, Takashi
AU - Kitamura, Kei Ichiro
AU - Endo, Masato
AU - Kobayashi, Isao
AU - Mishima, Hiroyuki
AU - Sekiguchi, Toshio
AU - Urata, Makoto
AU - Seki, Azusa
AU - Yano, Sachiko
AU - Hattori, Atsuhiko
AU - Suzuki, Nobuo
N1 - Publisher Copyright:
© 2017 Biomedical Research Foundation. All rights reserved.
PY - 2017
Y1 - 2017
N2 - The effects of low-intensity pulsed ultrasound (LIPUS) on osteoclastogenesis were examined using fish scales that had both osteoclasts and osteoblasts. The binding of the receptor activator of NF-κB ligand (RANKL) in osteoblasts to the receptor activator of NF-κB (RANK) in osteoclasts induced osteoclastogenesis. Therefore, we focused on RANK/RANKL signaling. After 6 h of incubation following LIPUS treatment, mRNA expression of RANKL increased significantly. Resulting from the increased RANKL mRNA level, the expression of transcription-regulating factors significantly increased after 6 h of incubation, and then the mRNA expression of functional genes was significantly up-regulated after 12 h of incubation. However, the mRNA expression of osteoprotegerin (OPG), which is known as an osteoclastogenesis inhibitory factor, also significantly increased after 6 h of incubation and tended to further increase after 12 h of incubation. At 24 h of incubation, osteoclastic functional genes’ mRNA expression decreased to the level of the control. Furthermore, we performed an in vivo experiment with goldfish. Two weeks after daily LIPUS exposure, osteoclastic marker enzymes tended to decrease while osteoblastic marker enzymes were activated. The regeneration rate of the LIPUS-treated scales was significantly higher than that of the control scales. Thus, LIPUS moderately activates osteoclasts and induces bone formation.
AB - The effects of low-intensity pulsed ultrasound (LIPUS) on osteoclastogenesis were examined using fish scales that had both osteoclasts and osteoblasts. The binding of the receptor activator of NF-κB ligand (RANKL) in osteoblasts to the receptor activator of NF-κB (RANK) in osteoclasts induced osteoclastogenesis. Therefore, we focused on RANK/RANKL signaling. After 6 h of incubation following LIPUS treatment, mRNA expression of RANKL increased significantly. Resulting from the increased RANKL mRNA level, the expression of transcription-regulating factors significantly increased after 6 h of incubation, and then the mRNA expression of functional genes was significantly up-regulated after 12 h of incubation. However, the mRNA expression of osteoprotegerin (OPG), which is known as an osteoclastogenesis inhibitory factor, also significantly increased after 6 h of incubation and tended to further increase after 12 h of incubation. At 24 h of incubation, osteoclastic functional genes’ mRNA expression decreased to the level of the control. Furthermore, we performed an in vivo experiment with goldfish. Two weeks after daily LIPUS exposure, osteoclastic marker enzymes tended to decrease while osteoblastic marker enzymes were activated. The regeneration rate of the LIPUS-treated scales was significantly higher than that of the control scales. Thus, LIPUS moderately activates osteoclasts and induces bone formation.
UR - http://www.scopus.com/inward/record.url?scp=85013652349&partnerID=8YFLogxK
U2 - 10.2220/biomedres.38.71
DO - 10.2220/biomedres.38.71
M3 - 学術論文
C2 - 28239034
AN - SCOPUS:85013652349
SN - 0388-6107
VL - 38
SP - 71
EP - 77
JO - Biomedical Research (Japan)
JF - Biomedical Research (Japan)
IS - 1
ER -