Effect of thrombin on the production of glycosaminoglycans by cultured endothelial cells

Toshiyuki Kaji; Takuya Akai; Tomohiro Hayashi; Nobuo Sakuragawa

doi:10.1016/0049-3848(91)90024-Q

Effect of thrombin on the production of glycosaminoglycans by cultured endothelial cells

Toshiyuki Kaji^*, Takuya Akai, Tomohiro Hayashi, Nobuo Sakuragawa

^*Corresponding author for this work

Neurosurgery

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

We investigated the effect of thrombin on the production process of glycosaminoglycans (GAG) in cultured bovine aortic endothelial cells. It was revealed that the trichloroacetic acid-insoluble [³⁵S]sulfate-labeled GAG (³⁵S-GAG) was decreased by thrombin. The thrombin-induced decrease in the accumulation of ³⁵S-GAG in the cell layer occurred even in the presence of actinomycin D or cycloheximide. The incorporation of both [³⁵S]sulfate and [³H]glucosamine into the GAG was decreased by thrombin, while the ratio of ³⁵S to ³H was not changed. Also, the incorporation of both [³H]glucosamine and [¹⁴C]UDP-xylose into the GAG was inhibited by thrombin. From these results, it was suggested that thrombin decreased GAG in the endothelial cell layer through an suppression of formation of polysaccharide chains rather than that of core protein.

Original language	English
Pages (from-to)	509-517
Number of pages	9
Journal	Thrombosis Research
Volume	62
Issue number	5
DOIs	https://doi.org/10.1016/0049-3848(91)90024-Q
State	Published - 1991/06/01

Keywords

Endothelial cells
Endothelium
Glycosaminoglycans
Thrombin

ASJC Scopus subject areas

Hematology

Access to Document

10.1016/0049-3848(91)90024-Q

Cite this

@article{6661060675f84a3ba0c45404bfcd3425,

title = "Effect of thrombin on the production of glycosaminoglycans by cultured endothelial cells",

abstract = "We investigated the effect of thrombin on the production process of glycosaminoglycans (GAG) in cultured bovine aortic endothelial cells. It was revealed that the trichloroacetic acid-insoluble [35S]sulfate-labeled GAG (35S-GAG) was decreased by thrombin. The thrombin-induced decrease in the accumulation of 35S-GAG in the cell layer occurred even in the presence of actinomycin D or cycloheximide. The incorporation of both [35S]sulfate and [3H]glucosamine into the GAG was decreased by thrombin, while the ratio of 35S to 3H was not changed. Also, the incorporation of both [3H]glucosamine and [14C]UDP-xylose into the GAG was inhibited by thrombin. From these results, it was suggested that thrombin decreased GAG in the endothelial cell layer through an suppression of formation of polysaccharide chains rather than that of core protein.",

keywords = "Endothelial cells, Endothelium, Glycosaminoglycans, Thrombin",

author = "Toshiyuki Kaji and Takuya Akai and Tomohiro Hayashi and Nobuo Sakuragawa",

year = "1991",

month = jun,

day = "1",

doi = "10.1016/0049-3848(91)90024-Q",

language = "英語",

volume = "62",

pages = "509--517",

journal = "Thrombosis Research",

issn = "0049-3848",

publisher = "Elsevier Ltd.",

number = "5",

}

TY - JOUR

T1 - Effect of thrombin on the production of glycosaminoglycans by cultured endothelial cells

AU - Kaji, Toshiyuki

AU - Akai, Takuya

AU - Hayashi, Tomohiro

AU - Sakuragawa, Nobuo

PY - 1991/6/1

Y1 - 1991/6/1

N2 - We investigated the effect of thrombin on the production process of glycosaminoglycans (GAG) in cultured bovine aortic endothelial cells. It was revealed that the trichloroacetic acid-insoluble [35S]sulfate-labeled GAG (35S-GAG) was decreased by thrombin. The thrombin-induced decrease in the accumulation of 35S-GAG in the cell layer occurred even in the presence of actinomycin D or cycloheximide. The incorporation of both [35S]sulfate and [3H]glucosamine into the GAG was decreased by thrombin, while the ratio of 35S to 3H was not changed. Also, the incorporation of both [3H]glucosamine and [14C]UDP-xylose into the GAG was inhibited by thrombin. From these results, it was suggested that thrombin decreased GAG in the endothelial cell layer through an suppression of formation of polysaccharide chains rather than that of core protein.

AB - We investigated the effect of thrombin on the production process of glycosaminoglycans (GAG) in cultured bovine aortic endothelial cells. It was revealed that the trichloroacetic acid-insoluble [35S]sulfate-labeled GAG (35S-GAG) was decreased by thrombin. The thrombin-induced decrease in the accumulation of 35S-GAG in the cell layer occurred even in the presence of actinomycin D or cycloheximide. The incorporation of both [35S]sulfate and [3H]glucosamine into the GAG was decreased by thrombin, while the ratio of 35S to 3H was not changed. Also, the incorporation of both [3H]glucosamine and [14C]UDP-xylose into the GAG was inhibited by thrombin. From these results, it was suggested that thrombin decreased GAG in the endothelial cell layer through an suppression of formation of polysaccharide chains rather than that of core protein.

KW - Endothelial cells

KW - Endothelium

KW - Glycosaminoglycans

KW - Thrombin

UR - http://www.scopus.com/inward/record.url?scp=0025809296&partnerID=8YFLogxK

U2 - 10.1016/0049-3848(91)90024-Q

DO - 10.1016/0049-3848(91)90024-Q

M3 - 学術論文

C2 - 1896966

AN - SCOPUS:0025809296

SN - 0049-3848

VL - 62

SP - 509

EP - 517

JO - Thrombosis Research

JF - Thrombosis Research

IS - 5

ER -

Effect of thrombin on the production of glycosaminoglycans by cultured endothelial cells

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Fingerprint

Cite this