Effect of pituitary adenylate cyclase-activating polypeptide (PACAP) on prolactin and somatolactin release from the goldfish pituitary in vitro

Kouhei Matsuda; Yumika Nejigaki; Misuzu Satoh; Chika Shimaura; Mio Tanaka; Keiichi Kawamoto; Minoru Uchiyama; Hiroshi Kawauchi; Seiji Shioda; Akiyoshi Takahashi

doi:10.1016/j.regpep.2007.08.018

Effect of pituitary adenylate cyclase-activating polypeptide (PACAP) on prolactin and somatolactin release from the goldfish pituitary in vitro

Kouhei Matsuda^*, Yumika Nejigaki, Misuzu Satoh, Chika Shimaura, Mio Tanaka, Keiichi Kawamoto, Minoru Uchiyama, Hiroshi Kawauchi, Seiji Shioda, Akiyoshi Takahashi

^*Corresponding author for this work

Department of Biology

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Pituitary adenylate cyclase-activating polypeptide (PACAP) plays a role in mediating growth hormone and gonadotropin release in the teleost pituitary. In the present study, we examined the immunohistochemical relationship between PACAP nerve fibers and prolactin (PRL)- and somatolactin (SL)-producing cells in the goldfish pituitary. Nerve fibers with PACAP-like immunoreactivity (PACAP-LI) were identified in the neurohypophysis in close proximity to cells containing PRL-LI or SL-LI. Several cells with PRL-LI or SL-LI showed PACAP receptor (PAC₁R)-LI. The cell immunoblot assay method was used to examine the effect of PACAP on PRL and SL release from dispersed goldfish pituitary cells. Treatment with PACAP increased the immunoblot area for PRL- and SL-LI from individual pituitary cells in a dose-dependent manner. The effect of PACAP on the expression of mRNAs for PRL and SL in cultured pituitary cells was also tested. Semiquantitative analysis revealed that the expression of SL mRNA, but not PRL mRNA, was increased significantly by the treatment with PACAP. The effect of PACAP on intracellular calcium mobilization in isolated pituitary cells was also investigated using confocal laser-scanning microscopy. The amplitude of Ca²⁺ mobilization in individual cells showing PRL- or SL-LI was increased significantly following exposure of cells to PACAP. These results indicate that PACAP can potentially function as a hypophysiotropic factor mediating PRL and SL release in the goldfish pituitary.

Original language	English
Pages (from-to)	72-79
Number of pages	8
Journal	Regulatory Peptides
Volume	145
Issue number	1-3
DOIs	https://doi.org/10.1016/j.regpep.2007.08.018
State	Published - 2008/01/10

Keywords

Goldfish
PACAP
Pituitary
Prolactin
Somatolactin

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Clinical Biochemistry
Cellular and Molecular Neuroscience

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10.1016/j.regpep.2007.08.018

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@article{eb5902242d4241f88df304f5937da24c,

title = "Effect of pituitary adenylate cyclase-activating polypeptide (PACAP) on prolactin and somatolactin release from the goldfish pituitary in vitro",

abstract = "Pituitary adenylate cyclase-activating polypeptide (PACAP) plays a role in mediating growth hormone and gonadotropin release in the teleost pituitary. In the present study, we examined the immunohistochemical relationship between PACAP nerve fibers and prolactin (PRL)- and somatolactin (SL)-producing cells in the goldfish pituitary. Nerve fibers with PACAP-like immunoreactivity (PACAP-LI) were identified in the neurohypophysis in close proximity to cells containing PRL-LI or SL-LI. Several cells with PRL-LI or SL-LI showed PACAP receptor (PAC1R)-LI. The cell immunoblot assay method was used to examine the effect of PACAP on PRL and SL release from dispersed goldfish pituitary cells. Treatment with PACAP increased the immunoblot area for PRL- and SL-LI from individual pituitary cells in a dose-dependent manner. The effect of PACAP on the expression of mRNAs for PRL and SL in cultured pituitary cells was also tested. Semiquantitative analysis revealed that the expression of SL mRNA, but not PRL mRNA, was increased significantly by the treatment with PACAP. The effect of PACAP on intracellular calcium mobilization in isolated pituitary cells was also investigated using confocal laser-scanning microscopy. The amplitude of Ca2+ mobilization in individual cells showing PRL- or SL-LI was increased significantly following exposure of cells to PACAP. These results indicate that PACAP can potentially function as a hypophysiotropic factor mediating PRL and SL release in the goldfish pituitary.",

keywords = "Goldfish, PACAP, Pituitary, Prolactin, Somatolactin",

author = "Kouhei Matsuda and Yumika Nejigaki and Misuzu Satoh and Chika Shimaura and Mio Tanaka and Keiichi Kawamoto and Minoru Uchiyama and Hiroshi Kawauchi and Seiji Shioda and Akiyoshi Takahashi",

note = "Funding Information: This work was supported in part by Grants-in-Aid from the ministry of Education, Culture, Sports, Science and Technology of Japan (K.M. and A.T.), and by research grants from Faculty of Science, University of Toyama (K.M.) and from the Toyama Marine Biotechnology Association (K.M.).",

year = "2008",

month = jan,

day = "10",

doi = "10.1016/j.regpep.2007.08.018",

language = "英語",

volume = "145",

pages = "72--79",

journal = "Regulatory Peptides",

issn = "0167-0115",

publisher = "Elsevier",

number = "1-3",

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Matsuda, K, Nejigaki, Y, Satoh, M, Shimaura, C, Tanaka, M, Kawamoto, K, Uchiyama, M, Kawauchi, H, Shioda, S & Takahashi, A 2008, 'Effect of pituitary adenylate cyclase-activating polypeptide (PACAP) on prolactin and somatolactin release from the goldfish pituitary in vitro', Regulatory Peptides, vol. 145, no. 1-3, pp. 72-79. https://doi.org/10.1016/j.regpep.2007.08.018

TY - JOUR

T1 - Effect of pituitary adenylate cyclase-activating polypeptide (PACAP) on prolactin and somatolactin release from the goldfish pituitary in vitro

AU - Matsuda, Kouhei

AU - Nejigaki, Yumika

AU - Satoh, Misuzu

AU - Shimaura, Chika

AU - Tanaka, Mio

AU - Kawamoto, Keiichi

AU - Uchiyama, Minoru

AU - Kawauchi, Hiroshi

AU - Shioda, Seiji

AU - Takahashi, Akiyoshi

N1 - Funding Information: This work was supported in part by Grants-in-Aid from the ministry of Education, Culture, Sports, Science and Technology of Japan (K.M. and A.T.), and by research grants from Faculty of Science, University of Toyama (K.M.) and from the Toyama Marine Biotechnology Association (K.M.).

PY - 2008/1/10

Y1 - 2008/1/10

N2 - Pituitary adenylate cyclase-activating polypeptide (PACAP) plays a role in mediating growth hormone and gonadotropin release in the teleost pituitary. In the present study, we examined the immunohistochemical relationship between PACAP nerve fibers and prolactin (PRL)- and somatolactin (SL)-producing cells in the goldfish pituitary. Nerve fibers with PACAP-like immunoreactivity (PACAP-LI) were identified in the neurohypophysis in close proximity to cells containing PRL-LI or SL-LI. Several cells with PRL-LI or SL-LI showed PACAP receptor (PAC1R)-LI. The cell immunoblot assay method was used to examine the effect of PACAP on PRL and SL release from dispersed goldfish pituitary cells. Treatment with PACAP increased the immunoblot area for PRL- and SL-LI from individual pituitary cells in a dose-dependent manner. The effect of PACAP on the expression of mRNAs for PRL and SL in cultured pituitary cells was also tested. Semiquantitative analysis revealed that the expression of SL mRNA, but not PRL mRNA, was increased significantly by the treatment with PACAP. The effect of PACAP on intracellular calcium mobilization in isolated pituitary cells was also investigated using confocal laser-scanning microscopy. The amplitude of Ca2+ mobilization in individual cells showing PRL- or SL-LI was increased significantly following exposure of cells to PACAP. These results indicate that PACAP can potentially function as a hypophysiotropic factor mediating PRL and SL release in the goldfish pituitary.

AB - Pituitary adenylate cyclase-activating polypeptide (PACAP) plays a role in mediating growth hormone and gonadotropin release in the teleost pituitary. In the present study, we examined the immunohistochemical relationship between PACAP nerve fibers and prolactin (PRL)- and somatolactin (SL)-producing cells in the goldfish pituitary. Nerve fibers with PACAP-like immunoreactivity (PACAP-LI) were identified in the neurohypophysis in close proximity to cells containing PRL-LI or SL-LI. Several cells with PRL-LI or SL-LI showed PACAP receptor (PAC1R)-LI. The cell immunoblot assay method was used to examine the effect of PACAP on PRL and SL release from dispersed goldfish pituitary cells. Treatment with PACAP increased the immunoblot area for PRL- and SL-LI from individual pituitary cells in a dose-dependent manner. The effect of PACAP on the expression of mRNAs for PRL and SL in cultured pituitary cells was also tested. Semiquantitative analysis revealed that the expression of SL mRNA, but not PRL mRNA, was increased significantly by the treatment with PACAP. The effect of PACAP on intracellular calcium mobilization in isolated pituitary cells was also investigated using confocal laser-scanning microscopy. The amplitude of Ca2+ mobilization in individual cells showing PRL- or SL-LI was increased significantly following exposure of cells to PACAP. These results indicate that PACAP can potentially function as a hypophysiotropic factor mediating PRL and SL release in the goldfish pituitary.

KW - Goldfish

KW - PACAP

KW - Pituitary

KW - Prolactin

KW - Somatolactin

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U2 - 10.1016/j.regpep.2007.08.018

DO - 10.1016/j.regpep.2007.08.018

M3 - 学術論文

C2 - 17920707

AN - SCOPUS:37349111559

SN - 0167-0115

VL - 145

SP - 72

EP - 79

JO - Regulatory Peptides

JF - Regulatory Peptides

IS - 1-3

ER -

Effect of pituitary adenylate cyclase-activating polypeptide (PACAP) on prolactin and somatolactin release from the goldfish pituitary in vitro

Abstract

Keywords

ASJC Scopus subject areas

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