Early detection of virus infection in live human cells using Raman spectroscopy

Kamila Moor; Yusuke Terada; Akinori Taketani; Hiroko Matsuyoshi; Kiyoshi Ohtani; Hidetoshi Sato

doi:10.1117/1.JBO.23.9.097001

Early detection of virus infection in live human cells using Raman spectroscopy

Kamila Moor, Yusuke Terada, Akinori Taketani, Hiroko Matsuyoshi, Kiyoshi Ohtani, Hidetoshi Sato^*

^*Corresponding author for this work

Research Center for Pre-Disease Science

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Virus infection of a human cell was determined only 3 h after invagination. We used viral vector Ad-CMV-control (AdC), which lacks the E1 gene coding for early polypeptide 1 (E1). AdC can replicate in human embryonic kidney 293 (HEK293) cells into which the E1 gene has been transfected. According to partial least-square regression discriminant analysis, it was assumed that two kinds of reaction take place in the cell during viral invasion. The first response of the cell was determined 3 h after the virus invasion, and the second one was determined ∼9 h later. The first one seems to be due to compositional changes in DNA. Analysis of large-scale datasets strongly indicated that the second reaction can be attributed to a reduction in protein concentration or uptake of phenylalanine into the nucleus.

Original language	English
Article number	097001
Journal	Journal of Biomedical Optics
Volume	23
Issue number	9
DOIs	https://doi.org/10.1117/1.JBO.23.9.097001
State	Published - 2018/09/01

Keywords

Raman spectroscopy
infection
virus

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Biomedical Engineering

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10.1117/1.JBO.23.9.097001

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title = "Early detection of virus infection in live human cells using Raman spectroscopy",

abstract = "Virus infection of a human cell was determined only 3 h after invagination. We used viral vector Ad-CMV-control (AdC), which lacks the E1 gene coding for early polypeptide 1 (E1). AdC can replicate in human embryonic kidney 293 (HEK293) cells into which the E1 gene has been transfected. According to partial least-square regression discriminant analysis, it was assumed that two kinds of reaction take place in the cell during viral invasion. The first response of the cell was determined 3 h after the virus invasion, and the second one was determined ∼9 h later. The first one seems to be due to compositional changes in DNA. Analysis of large-scale datasets strongly indicated that the second reaction can be attributed to a reduction in protein concentration or uptake of phenylalanine into the nucleus.",

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author = "Kamila Moor and Yusuke Terada and Akinori Taketani and Hiroko Matsuyoshi and Kiyoshi Ohtani and Hidetoshi Sato",

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T1 - Early detection of virus infection in live human cells using Raman spectroscopy

AU - Moor, Kamila

AU - Terada, Yusuke

AU - Taketani, Akinori

AU - Matsuyoshi, Hiroko

AU - Ohtani, Kiyoshi

AU - Sato, Hidetoshi

PY - 2018/9/1

Y1 - 2018/9/1

N2 - Virus infection of a human cell was determined only 3 h after invagination. We used viral vector Ad-CMV-control (AdC), which lacks the E1 gene coding for early polypeptide 1 (E1). AdC can replicate in human embryonic kidney 293 (HEK293) cells into which the E1 gene has been transfected. According to partial least-square regression discriminant analysis, it was assumed that two kinds of reaction take place in the cell during viral invasion. The first response of the cell was determined 3 h after the virus invasion, and the second one was determined ∼9 h later. The first one seems to be due to compositional changes in DNA. Analysis of large-scale datasets strongly indicated that the second reaction can be attributed to a reduction in protein concentration or uptake of phenylalanine into the nucleus.

AB - Virus infection of a human cell was determined only 3 h after invagination. We used viral vector Ad-CMV-control (AdC), which lacks the E1 gene coding for early polypeptide 1 (E1). AdC can replicate in human embryonic kidney 293 (HEK293) cells into which the E1 gene has been transfected. According to partial least-square regression discriminant analysis, it was assumed that two kinds of reaction take place in the cell during viral invasion. The first response of the cell was determined 3 h after the virus invasion, and the second one was determined ∼9 h later. The first one seems to be due to compositional changes in DNA. Analysis of large-scale datasets strongly indicated that the second reaction can be attributed to a reduction in protein concentration or uptake of phenylalanine into the nucleus.

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Early detection of virus infection in live human cells using Raman spectroscopy

Abstract

Keywords

ASJC Scopus subject areas

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