TY - JOUR
T1 - DNMT1 Expression and DNA Methylation in Intraductal Papillary Neoplasms of the Bile Duct
AU - Doi, Reiko
AU - Fukumura, Yuki
AU - Lu, Rong
AU - Hirabayashi, Kenichi
AU - Kinowaki, Yuko
AU - Nakanuma, Yasuni
AU - Kanai, Yoshiakira
AU - Nakahodo, Jun
AU - Sasahara, Noriko
AU - Saito, Tsuyoshi
AU - Yao, Takashi
N1 - Publisher Copyright:
© 2022 International Institute of Anticancer Research. All rights reserved.
PY - 2022/6
Y1 - 2022/6
N2 - Background/Aim: Intraductal papillary neoplasms of the bile duct (IPNB) are histologically and clinically classified as type 1 and 2. This study aimed to identify the differences between these two types. Materials and Methods: Based on multiple gene expression analysis (MGEA) using type 1, type 2, and pancreatic intraductal papillary mucinous neoplasms (n=4, 6, and 5, respectively), immunohistochemistry of DNMT1 and methylation-specific PCR for p16, APC, BRCA1, hMLH1, TIMP3, and SOX17 were performed on type 1 and 2 IPNBs (n=14, each). Results: The DNMT1 protein was highly expressed (p<0.001) in 28.6% of type 1 cases and all type 2 cases. The DNA methylation ratio for the six genes in total as well as for SOX17 was lower in type 1 than in type 2 (p<0.05 each). Conclusion: Type 2 IPNB showed increased DNMT1 protein expression and increased DNA methylation frequency of the examined tumor suppressor genes compared to type 1. DNMT1 IHC may be helpful in discriminating between these two types.
AB - Background/Aim: Intraductal papillary neoplasms of the bile duct (IPNB) are histologically and clinically classified as type 1 and 2. This study aimed to identify the differences between these two types. Materials and Methods: Based on multiple gene expression analysis (MGEA) using type 1, type 2, and pancreatic intraductal papillary mucinous neoplasms (n=4, 6, and 5, respectively), immunohistochemistry of DNMT1 and methylation-specific PCR for p16, APC, BRCA1, hMLH1, TIMP3, and SOX17 were performed on type 1 and 2 IPNBs (n=14, each). Results: The DNMT1 protein was highly expressed (p<0.001) in 28.6% of type 1 cases and all type 2 cases. The DNA methylation ratio for the six genes in total as well as for SOX17 was lower in type 1 than in type 2 (p<0.05 each). Conclusion: Type 2 IPNB showed increased DNMT1 protein expression and increased DNA methylation frequency of the examined tumor suppressor genes compared to type 1. DNMT1 IHC may be helpful in discriminating between these two types.
KW - DNA methylation
KW - DNMT1
KW - Intraductal papillary neoplasm type 1
KW - intraductal papillary neoplasm type 2
KW - multiple gene expression analysis
UR - http://www.scopus.com/inward/record.url?scp=85131192210&partnerID=8YFLogxK
U2 - 10.21873/anticanres.15771
DO - 10.21873/anticanres.15771
M3 - 学術論文
C2 - 35641291
AN - SCOPUS:85131192210
SN - 0250-7005
VL - 42
SP - 2893
EP - 2902
JO - Anticancer Research
JF - Anticancer Research
IS - 6
ER -
