Differential cytotoxicity and sonosensitization by sanazole: Effect of cell type and acoustic parameters

Mariame A. Hassan, Yukihiro Furusawa, Qing Li Zhao, Ichiro Takasaki, Loreto B. Feril, Katsuro Tachibana, Nobuki Kudo, Masami Minemura, Toshiro Sugiyama, Takashi Kondo*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Purpose: Although sanazole has been used as a hypoxic radiosensitizer, we recently reported on its ability to sensitize U937 cells to hyperthermia and X-irradiation under aerobic conditions, enhancing apoptotic cell death following the combined treatment. The current study was undertaken to evaluate the effect of sanazole as a sonosensitizer under previously studied acoustic conditions of different pulse repetition frequencies, using two cell lines representative of solid tumours and haematopoietic cancers. Methods: Cells were treated with different doses of sanazole. Flow-cytometric analysis and DNA fragmentation assay were carried out at different times, and morphological features were also inspected. For ultrasound treatment, cells were pre-incubated with a non-cytotoxic dose of sanazole for 30 min before exposure. Evaluation of cell killing and a parallel examination of intracellular oxidative stress levels in both cell lines were performed using flow cytometry. Results: Sanazole alone displayed selective cytotoxic effects towards solid tumour-derived cancer cells, resulting in complete cell death after 24 h of treatment, and enhanced the ultrasound-induced cell killing 6 h post-treatment. The enhancement seemed to be mediated by an additive increase in intracellular oxidative stress levels. Conclusion: Sanazole seems to be an efficient cytotoxic agent for the treatment of solid tumours and a promising sonosensitizer under aerobic conditions.

Original languageEnglish
Pages (from-to)65-72
Number of pages8
JournalJournal of Medical Ultrasonics
Volume38
Issue number2
DOIs
StatePublished - 2011/04

Keywords

  • Apoptosis
  • Pulse repetition frequency
  • Sanazole
  • Sonosensitizer
  • Ultrasound

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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