TY - JOUR
T1 - Dicer has a crucial role in the early stage of adipocyte differentiation, but not in lipid synthesis, in 3T3-L1 cells
AU - Fujimoto, Yuri
AU - Nakagawa, Yoshimi
AU - Shingyouchi, Akiko
AU - Tokushige, Naoko
AU - Nakanishi, Noriko
AU - Satoh, Aoi
AU - Matsuzaka, Takashi
AU - Ishii, Kiyo aki
AU - Iwasaki, Hitoshi
AU - Kobayashi, Kazuto
AU - Yatoh, Shigeru
AU - Suzuki, Hiroaki
AU - Yahagi, Naoya
AU - Urayama, Osamu
AU - Yamada, Nobuhiro
AU - Shimano, Hitoshi
N1 - Funding Information:
We thank F Miyamasu for critical reading of the manuscript. This work was supported by Grants-in-Aid from The Japanese Ministry of Science, Education, Culture and Technology .
PY - 2012/4/20
Y1 - 2012/4/20
N2 - Dicer is a rate-limiting enzyme for microRNA (miRNA) synthesis. To determine the effects of Dicer on adipogenesis, we performed stage-specific knockdown of Dicer using adenovirus encoding short-hairpin RNAi against Dicer in 3T3-L1 cells. When cells were infected with the adenovirus before induction of adipocyte differentiation, Dicer RNAi suppressed the gene expression of inducers of adipocyte differentiation such as PPARγ, C/EBPα, and FAS in 3T3-L1 cells during adipocyte differentiation. Concurrently, both adipocyte differentiation and cellular lipid accumulation were cancelled by Dicer RNAi when compared with control RNAi. Meanwhile, we addressed the roles of Dicer in lipid synthesis and accumulation in the final stages of differentiation. When the differentiated cells at day 4 after induction of differentiation were infected with adenovirus Dicer RNAi, cellular lipid accumulation was unchanged. Consistent with this, Dicer RNAi had no effects on the expression of genes related to cellular lipid accumulation, including PPARγ and FAS. Thus, Dicer controls proadipogenic genes such as C/EBPα and PPARγ in the early, but not in the late, stage of adipogenesis via regulation of miRNA synthesis.
AB - Dicer is a rate-limiting enzyme for microRNA (miRNA) synthesis. To determine the effects of Dicer on adipogenesis, we performed stage-specific knockdown of Dicer using adenovirus encoding short-hairpin RNAi against Dicer in 3T3-L1 cells. When cells were infected with the adenovirus before induction of adipocyte differentiation, Dicer RNAi suppressed the gene expression of inducers of adipocyte differentiation such as PPARγ, C/EBPα, and FAS in 3T3-L1 cells during adipocyte differentiation. Concurrently, both adipocyte differentiation and cellular lipid accumulation were cancelled by Dicer RNAi when compared with control RNAi. Meanwhile, we addressed the roles of Dicer in lipid synthesis and accumulation in the final stages of differentiation. When the differentiated cells at day 4 after induction of differentiation were infected with adenovirus Dicer RNAi, cellular lipid accumulation was unchanged. Consistent with this, Dicer RNAi had no effects on the expression of genes related to cellular lipid accumulation, including PPARγ and FAS. Thus, Dicer controls proadipogenic genes such as C/EBPα and PPARγ in the early, but not in the late, stage of adipogenesis via regulation of miRNA synthesis.
KW - Adipocyte differentiation
KW - Adipogenesis
KW - Dicer
KW - Lipid synthesis
KW - MicroRNA
UR - http://www.scopus.com/inward/record.url?scp=84859817602&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2012.03.110
DO - 10.1016/j.bbrc.2012.03.110
M3 - 学術論文
C2 - 22475483
AN - SCOPUS:84859817602
SN - 0006-291X
VL - 420
SP - 931
EP - 936
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -