Development of reference material with assigned value for human T-cell leukemia virus type 1 quantitative PCR in Japan

Madoka Kuramitsu; Kazu Okuma; Makoto Nakashima; Tomoo Sato; Daisuke Sasaki; Hiroo Hasegawa; Kazumi Umeki; Ryuji Kubota; Keiko Sasada; Rieko Sobata; Chieko Matsumoto; Noriaki Kaneko; Kenta Tezuka; Sahoko Matsuoka; Atae Utsunomiya; Ki Ryang Koh; Masao Ogata; Kenji Ishitsuka; Mai Taki; Kisato Nosaka; Kaoru Uchimaru; Masako Iwanaga; Yasuko Sagara; Yoshihisa Yamano; Akihiko Okayama; Kiyonori Miura; Masahiro Satake; Shigeru Saito; Toshiki Watanabe; Isao Hamaguchi

doi:10.1111/1348-0421.12644

Development of reference material with assigned value for human T-cell leukemia virus type 1 quantitative PCR in Japan

Madoka Kuramitsu, Kazu Okuma, Makoto Nakashima, Tomoo Sato, Daisuke Sasaki, Hiroo Hasegawa, Kazumi Umeki, Ryuji Kubota, Keiko Sasada, Rieko Sobata, Chieko Matsumoto, Noriaki Kaneko, Kenta Tezuka, Sahoko Matsuoka, Atae Utsunomiya, Ki Ryang Koh, Masao Ogata, Kenji Ishitsuka, Mai Taki, Kisato NosakaKaoru Uchimaru, Masako Iwanaga, Yasuko Sagara, Yoshihisa Yamano, Akihiko Okayama, Kiyonori Miura, Masahiro Satake, Shigeru Saito, Toshiki Watanabe, Isao Hamaguchi^*

^*Corresponding author for this work

Member of the Board

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Quantitative PCR (qPCR) of human T-cell leukemia virus type 1 (HTLV-1) provirus is used for HTLV-1 testing and for assessment of risk of HTLV-1-related diseases. In this study, a reference material was developed for standardizing HTLV-1 qPCR. Freeze-dried TL-Om1 cells diluted with Jurkat cells were prepared and an assigned value for proviral load (PVL) of 2.71 copies/100 cells was determined by digital PCR. Nine Japanese laboratories using their own methods evaluated the PVLs of this reference material as 1.08–3.49 copies/100 cells. The maximum difference between laboratories was 3.2-fold. Correcting measured PVLs by using a formula incorporating the assigned value of this reference material should minimize such discrepancies.

Original language	English
Pages (from-to)	673-676
Number of pages	4
Journal	Microbiology and Immunology
Volume	62
Issue number	10
DOIs	https://doi.org/10.1111/1348-0421.12644
State	Published - 2018/10

Keywords

human T-cell leukemia virus type 1
proviral load
quantitative PCR
standard

ASJC Scopus subject areas

Microbiology
Immunology
Virology

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Kuramitsu, M., Okuma, K., Nakashima, M., Sato, T., Sasaki, D., Hasegawa, H., Umeki, K., Kubota, R., Sasada, K., Sobata, R., Matsumoto, C., Kaneko, N., Tezuka, K., Matsuoka, S., Utsunomiya, A., Koh, K. R., Ogata, M., Ishitsuka, K., Taki, M., ... Hamaguchi, I. (2018). Development of reference material with assigned value for human T-cell leukemia virus type 1 quantitative PCR in Japan. Microbiology and Immunology, 62(10), 673-676. https://doi.org/10.1111/1348-0421.12644

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title = "Development of reference material with assigned value for human T-cell leukemia virus type 1 quantitative PCR in Japan",

abstract = "Quantitative PCR (qPCR) of human T-cell leukemia virus type 1 (HTLV-1) provirus is used for HTLV-1 testing and for assessment of risk of HTLV-1-related diseases. In this study, a reference material was developed for standardizing HTLV-1 qPCR. Freeze-dried TL-Om1 cells diluted with Jurkat cells were prepared and an assigned value for proviral load (PVL) of 2.71 copies/100 cells was determined by digital PCR. Nine Japanese laboratories using their own methods evaluated the PVLs of this reference material as 1.08–3.49 copies/100 cells. The maximum difference between laboratories was 3.2-fold. Correcting measured PVLs by using a formula incorporating the assigned value of this reference material should minimize such discrepancies.",

keywords = "human T-cell leukemia virus type 1, proviral load, quantitative PCR, standard",

author = "Madoka Kuramitsu and Kazu Okuma and Makoto Nakashima and Tomoo Sato and Daisuke Sasaki and Hiroo Hasegawa and Kazumi Umeki and Ryuji Kubota and Keiko Sasada and Rieko Sobata and Chieko Matsumoto and Noriaki Kaneko and Kenta Tezuka and Sahoko Matsuoka and Atae Utsunomiya and Koh, {Ki Ryang} and Masao Ogata and Kenji Ishitsuka and Mai Taki and Kisato Nosaka and Kaoru Uchimaru and Masako Iwanaga and Yasuko Sagara and Yoshihisa Yamano and Akihiko Okayama and Kiyonori Miura and Masahiro Satake and Shigeru Saito and Toshiki Watanabe and Isao Hamaguchi",

note = "Publisher Copyright: {\textcopyright} 2018 The Societies and John Wiley & Sons Australia, Ltd",

year = "2018",

month = oct,

doi = "10.1111/1348-0421.12644",

language = "英語",

volume = "62",

pages = "673--676",

journal = "Microbiology and Immunology",

issn = "0385-5600",

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Kuramitsu, M, Okuma, K, Nakashima, M, Sato, T, Sasaki, D, Hasegawa, H, Umeki, K, Kubota, R, Sasada, K, Sobata, R, Matsumoto, C, Kaneko, N, Tezuka, K, Matsuoka, S, Utsunomiya, A, Koh, KR, Ogata, M, Ishitsuka, K, Taki, M, Nosaka, K, Uchimaru, K, Iwanaga, M, Sagara, Y, Yamano, Y, Okayama, A, Miura, K, Satake, M, Saito, S, Watanabe, T & Hamaguchi, I 2018, 'Development of reference material with assigned value for human T-cell leukemia virus type 1 quantitative PCR in Japan', Microbiology and Immunology, vol. 62, no. 10, pp. 673-676. https://doi.org/10.1111/1348-0421.12644

TY - JOUR

T1 - Development of reference material with assigned value for human T-cell leukemia virus type 1 quantitative PCR in Japan

AU - Kuramitsu, Madoka

AU - Okuma, Kazu

AU - Nakashima, Makoto

AU - Sato, Tomoo

AU - Sasaki, Daisuke

AU - Hasegawa, Hiroo

AU - Umeki, Kazumi

AU - Kubota, Ryuji

AU - Sasada, Keiko

AU - Sobata, Rieko

AU - Matsumoto, Chieko

AU - Kaneko, Noriaki

AU - Tezuka, Kenta

AU - Matsuoka, Sahoko

AU - Utsunomiya, Atae

AU - Koh, Ki Ryang

AU - Ogata, Masao

AU - Ishitsuka, Kenji

AU - Taki, Mai

AU - Nosaka, Kisato

AU - Uchimaru, Kaoru

AU - Iwanaga, Masako

AU - Sagara, Yasuko

AU - Yamano, Yoshihisa

AU - Okayama, Akihiko

AU - Miura, Kiyonori

AU - Satake, Masahiro

AU - Saito, Shigeru

AU - Watanabe, Toshiki

AU - Hamaguchi, Isao

PY - 2018/10

Y1 - 2018/10

N2 - Quantitative PCR (qPCR) of human T-cell leukemia virus type 1 (HTLV-1) provirus is used for HTLV-1 testing and for assessment of risk of HTLV-1-related diseases. In this study, a reference material was developed for standardizing HTLV-1 qPCR. Freeze-dried TL-Om1 cells diluted with Jurkat cells were prepared and an assigned value for proviral load (PVL) of 2.71 copies/100 cells was determined by digital PCR. Nine Japanese laboratories using their own methods evaluated the PVLs of this reference material as 1.08–3.49 copies/100 cells. The maximum difference between laboratories was 3.2-fold. Correcting measured PVLs by using a formula incorporating the assigned value of this reference material should minimize such discrepancies.

AB - Quantitative PCR (qPCR) of human T-cell leukemia virus type 1 (HTLV-1) provirus is used for HTLV-1 testing and for assessment of risk of HTLV-1-related diseases. In this study, a reference material was developed for standardizing HTLV-1 qPCR. Freeze-dried TL-Om1 cells diluted with Jurkat cells were prepared and an assigned value for proviral load (PVL) of 2.71 copies/100 cells was determined by digital PCR. Nine Japanese laboratories using their own methods evaluated the PVLs of this reference material as 1.08–3.49 copies/100 cells. The maximum difference between laboratories was 3.2-fold. Correcting measured PVLs by using a formula incorporating the assigned value of this reference material should minimize such discrepancies.

KW - human T-cell leukemia virus type 1

KW - proviral load

KW - quantitative PCR

KW - standard

UR - http://www.scopus.com/inward/record.url?scp=85055164355&partnerID=8YFLogxK

U2 - 10.1111/1348-0421.12644

DO - 10.1111/1348-0421.12644

M3 - 学術論文

C2 - 30125970

AN - SCOPUS:85055164355

SN - 0385-5600

VL - 62

SP - 673

EP - 676

JO - Microbiology and Immunology

JF - Microbiology and Immunology

IS - 10

ER -

Development of reference material with assigned value for human T-cell leukemia virus type 1 quantitative PCR in Japan

Abstract

Keywords

ASJC Scopus subject areas

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