Deterioration of apatite orientation in the cholecystokinin B receptor gene (Cckbr)-deficient mouse femurs

Yuki Mihara, Takuya Ishimoto, Ryosuke Ozasa, Takao Omura, Yu Yamato, Tomohiro Yamada, Ayako Okamoto, Yukihiro Matsuyama*, Takayoshi Nakano*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Introduction: The discrepancy between bone mineral density (BMD), the gold standard for bone assessment, and bone strength is a constraint in diagnosing bone function and determining treatment strategies for several bone diseases. Gastric hypochlorhydria induced by clinically used proton pump inhibitor (PPI) therapy indicates a discordance between changes in BMD and bone strength. Here, we used Cckbr-deficient mice with gastric hypochlorhydria to examine the effect of gastric hypochlorhydria on bone mass, BMD, and preferential orientation of the apatite crystallites, which is a strong indicator of bone strength. Materials and methods: Cckbr-deficient mice were created, and their femurs were analyzed for BMD and preferential orientation of the apatite c-axis along the femoral long axis. Results: Cckbr-deficient mouse femurs displayed a slight osteoporotic bone loss at 18 weeks of age; however, BMD was comparable to that of wild-type mice. In contrast, apatite orientation in the femur mid-shaft significantly decreased from 9 to 18 weeks. To the best of our knowledge, this is the first report demonstrating the deterioration of apatite orientation in the bones of Cckbr-deficient mice. Conclusion: Lesions in Cckbr-deficient mice occurred earlier in apatite orientation than in bone mass. Hence, bone apatite orientation may be a promising method for detecting hypochlorhydria-induced osteoporosis caused by PPI treatment and warrants urgent clinical applications.

Original languageEnglish
Pages (from-to)752-759
Number of pages8
JournalJournal of Bone and Mineral Metabolism
Volume41
Issue number6
DOIs
StatePublished - 2023/11

Keywords

  • Apatite orientation
  • Bone evaluation
  • Bone quality
  • Cckbr-deficient mice
  • Hypochlorhydria

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Orthopedics and Sports Medicine
  • Endocrinology

Fingerprint

Dive into the research topics of 'Deterioration of apatite orientation in the cholecystokinin B receptor gene (Cckbr)-deficient mouse femurs'. Together they form a unique fingerprint.

Cite this