Detection of mismatched duplexes by synchronizing the pulse potential frequency with the dynamics of ferrocene/isoquinoline conjugate-connected DNA probes immobilized onto electrodes

Reona Ikeda, Sakie Kobayashi, Junya Chiba, Masahiko Inouye*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

36 Citations (SciVal)

Abstract

Cyclic voltammetry was performed at various scan rates for the duplexes from ferrocene/isoquinoline conjugate-connected DNA probes on gold electrodes. The relationship between the observed currents and the scan rates disclosed the enhanced bending elasticity of the mismatched duplexes compared with the fully matched duplexes. The difference of the dynamics was easily detected through the currents from the conjugate by adjusting the pulse potential frequency in square-wave voltammetry. By using the present strategy, we succeeded in accurately detecting various naturally occurring single-nucleotide polymorphisms.

Original languageEnglish
Pages (from-to)4822-4828
Number of pages7
JournalChemistry - A European Journal
Volume15
Issue number19
DOIs
StatePublished - 2009/05/04

Keywords

  • Bending elasticity
  • DNA
  • Electrochemistry
  • Single nucleotide polymorphisms
  • Voltammetry

ASJC Scopus subject areas

  • Catalysis
  • General Chemistry
  • Organic Chemistry

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