Detection of HTLV‐I Genome in Seronegative Infants Born to HTLV‐I Seropositive Mothers by Polymerase Chain Reaction

Shigeru Saito; Yoshiya Ando; Kazuhiko Furuki; Kazuhiro Kakimoto; Takuo Tanigawa; Ikuko Moriyama; Motohiko Ichijo; Masataka Nakamura; Kiyoshi Ohtani; Kazuo Sugamura

doi:10.1111/j.1349-7006.1989.tb01718.x

Detection of HTLV‐I Genome in Seronegative Infants Born to HTLV‐I Seropositive Mothers by Polymerase Chain Reaction

Shigeru Saito^*, Yoshiya Ando, Kazuhiko Furuki, Kazuhiro Kakimoto, Takuo Tanigawa, Ikuko Moriyama, Motohiko Ichijo, Masataka Nakamura, Kiyoshi Ohtani, Kazuo Sugamura

^*Corresponding author for this work

Member of the Board

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

We applied the polymerase chain reaction (PCR) method to detect gag, env and pX sequences of human T cell leukemia virus type I (HTLV‐I) provirus in peripheral blood lymphocytes of seronega‐tive infants born to HTLV‐I seropositive mothers. Out of 22, five subjects were found to contain the HTLV‐I provirus genome. Two of the five cases were judged to be negative for not only anti‐HTLV‐I antibodies but also the viral antigens on cultivated lymphocytes by the conventional antibody/antigen detection methods. These results indicate that PCR is of great use as a simple and highly sensitive method detect HTLV‐I infection.

Original language	English
Pages (from-to)	808-812
Number of pages	5
Journal	Japanese Journal of Cancer Research
Volume	80
Issue number	9
DOIs	https://doi.org/10.1111/j.1349-7006.1989.tb01718.x
State	Published - 1989/09

Keywords

HTLV‐I
Polymerase chain reaction
Vertical infection

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1111/j.1349-7006.1989.tb01718.x

Cite this

@article{fa5c05861ec441f483fd35616ed359ec,

title = "Detection of HTLV‐I Genome in Seronegative Infants Born to HTLV‐I Seropositive Mothers by Polymerase Chain Reaction",

abstract = "We applied the polymerase chain reaction (PCR) method to detect gag, env and pX sequences of human T cell leukemia virus type I (HTLV‐I) provirus in peripheral blood lymphocytes of seronega‐tive infants born to HTLV‐I seropositive mothers. Out of 22, five subjects were found to contain the HTLV‐I provirus genome. Two of the five cases were judged to be negative for not only anti‐HTLV‐I antibodies but also the viral antigens on cultivated lymphocytes by the conventional antibody/antigen detection methods. These results indicate that PCR is of great use as a simple and highly sensitive method detect HTLV‐I infection.",

keywords = "HTLV‐I, Polymerase chain reaction, Vertical infection",

author = "Shigeru Saito and Yoshiya Ando and Kazuhiko Furuki and Kazuhiro Kakimoto and Takuo Tanigawa and Ikuko Moriyama and Motohiko Ichijo and Masataka Nakamura and Kiyoshi Ohtani and Kazuo Sugamura",

year = "1989",

month = sep,

doi = "10.1111/j.1349-7006.1989.tb01718.x",

language = "英語",

volume = "80",

pages = "808--812",

journal = "Japanese Journal of Cancer Research",

issn = "0910-5050",

publisher = "Oxford University Press",

number = "9",

}

TY - JOUR

T1 - Detection of HTLV‐I Genome in Seronegative Infants Born to HTLV‐I Seropositive Mothers by Polymerase Chain Reaction

AU - Saito, Shigeru

AU - Ando, Yoshiya

AU - Furuki, Kazuhiko

AU - Kakimoto, Kazuhiro

AU - Tanigawa, Takuo

AU - Moriyama, Ikuko

AU - Ichijo, Motohiko

AU - Nakamura, Masataka

AU - Ohtani, Kiyoshi

AU - Sugamura, Kazuo

PY - 1989/9

Y1 - 1989/9

N2 - We applied the polymerase chain reaction (PCR) method to detect gag, env and pX sequences of human T cell leukemia virus type I (HTLV‐I) provirus in peripheral blood lymphocytes of seronega‐tive infants born to HTLV‐I seropositive mothers. Out of 22, five subjects were found to contain the HTLV‐I provirus genome. Two of the five cases were judged to be negative for not only anti‐HTLV‐I antibodies but also the viral antigens on cultivated lymphocytes by the conventional antibody/antigen detection methods. These results indicate that PCR is of great use as a simple and highly sensitive method detect HTLV‐I infection.

AB - We applied the polymerase chain reaction (PCR) method to detect gag, env and pX sequences of human T cell leukemia virus type I (HTLV‐I) provirus in peripheral blood lymphocytes of seronega‐tive infants born to HTLV‐I seropositive mothers. Out of 22, five subjects were found to contain the HTLV‐I provirus genome. Two of the five cases were judged to be negative for not only anti‐HTLV‐I antibodies but also the viral antigens on cultivated lymphocytes by the conventional antibody/antigen detection methods. These results indicate that PCR is of great use as a simple and highly sensitive method detect HTLV‐I infection.

KW - HTLV‐I

KW - Polymerase chain reaction

KW - Vertical infection

UR - http://www.scopus.com/inward/record.url?scp=0024806990&partnerID=8YFLogxK

U2 - 10.1111/j.1349-7006.1989.tb01718.x

DO - 10.1111/j.1349-7006.1989.tb01718.x

M3 - 学術論文

C2 - 2513296

AN - SCOPUS:0024806990

SN - 0910-5050

VL - 80

SP - 808

EP - 812

JO - Japanese Journal of Cancer Research

JF - Japanese Journal of Cancer Research

IS - 9

ER -

Detection of HTLV‐I Genome in Seronegative Infants Born to HTLV‐I Seropositive Mothers by Polymerase Chain Reaction

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Fingerprint

Cite this