Cotranscription of the genes encoding two P700 chlorophyll a apoproteins with the gene for ribosomal protein CS14: determination of the transcriptional initiation site by in vitro capping

Bing Yuan Meng; Minoru Tanaka; Tatsuya Wakasugi; Masaru Ohme; Kazuo Shinozaki; Masahiro Sugiura

doi:10.1007/BF00419998

Cotranscription of the genes encoding two P700 chlorophyll a apoproteins with the gene for ribosomal protein CS14: determination of the transcriptional initiation site by in vitro capping

Bing Yuan Meng, Minoru Tanaka, Tatsuya Wakasugi, Masaru Ohme, Kazuo Shinozaki, Masahiro Sugiura^*

^*Corresponding author for this work

Department of Biology

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

Transcription of the psaA operon in tobacco chloroplasts has been studied. This operon contains in linear sequence the genes encoding the P₇₀₀ chlorophyll a A1 and A2 apoproteins (psaA and psaB) and the gene encoding the ribosomal CS14 protein (rps14). Northern blot hybridization revealed that a 5.2 kb transcript hybridizes to psaA, psaB, and rps14, but not to the fMet-tRNA (trnfM) gene which follows. Primer extension and in vitro capping assays indicated that the transcriptional initiation site is 194 by upstream of psaA. The 3′ end of the transcript was determined by S1 mapping to be 105 bp downstream of rps14. The transcript is calculated to be 5,207 nucleotides long.

Original language	English
Pages (from-to)	395-400
Number of pages	6
Journal	Current Genetics
Volume	14
Issue number	4
DOIs	https://doi.org/10.1007/BF00419998
State	Published - 1988/10

Keywords

Capping
Chloroplast
P chlorophyll a apoprotein gene
Ribosomal protein gene
Tobacco
Transcription

ASJC Scopus subject areas

Genetics

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10.1007/BF00419998

Cite this

@article{33c995504c344ddaa5e47aac643639f4,

title = "Cotranscription of the genes encoding two P700 chlorophyll a apoproteins with the gene for ribosomal protein CS14: determination of the transcriptional initiation site by in vitro capping",

abstract = "Transcription of the psaA operon in tobacco chloroplasts has been studied. This operon contains in linear sequence the genes encoding the P700 chlorophyll a A1 and A2 apoproteins (psaA and psaB) and the gene encoding the ribosomal CS14 protein (rps14). Northern blot hybridization revealed that a 5.2 kb transcript hybridizes to psaA, psaB, and rps14, but not to the fMet-tRNA (trnfM) gene which follows. Primer extension and in vitro capping assays indicated that the transcriptional initiation site is 194 by upstream of psaA. The 3′ end of the transcript was determined by S1 mapping to be 105 bp downstream of rps14. The transcript is calculated to be 5,207 nucleotides long.",

keywords = "Capping, Chloroplast, P chlorophyll a apoprotein gene, Ribosomal protein gene, Tobacco, Transcription",

author = "{Yuan Meng}, Bing and Minoru Tanaka and Tatsuya Wakasugi and Masaru Ohme and Kazuo Shinozaki and Masahiro Sugiura",

year = "1988",

month = oct,

doi = "10.1007/BF00419998",

language = "英語",

volume = "14",

pages = "395--400",

journal = "Current Genetics",

issn = "0172-8083",

publisher = "Springer Science and Business Media Deutschland GmbH",

number = "4",

}

Cotranscription of the genes encoding two P700 chlorophyll a apoproteins with the gene for ribosomal protein CS14: determination of the transcriptional initiation site by in vitro capping. / Yuan Meng, Bing; Tanaka, Minoru; Wakasugi, Tatsuya et al.
In: Current Genetics, Vol. 14, No. 4, 10.1988, p. 395-400.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Cotranscription of the genes encoding two P700 chlorophyll a apoproteins with the gene for ribosomal protein CS14

T2 - determination of the transcriptional initiation site by in vitro capping

AU - Yuan Meng, Bing

AU - Tanaka, Minoru

AU - Wakasugi, Tatsuya

AU - Ohme, Masaru

AU - Shinozaki, Kazuo

AU - Sugiura, Masahiro

PY - 1988/10

Y1 - 1988/10

N2 - Transcription of the psaA operon in tobacco chloroplasts has been studied. This operon contains in linear sequence the genes encoding the P700 chlorophyll a A1 and A2 apoproteins (psaA and psaB) and the gene encoding the ribosomal CS14 protein (rps14). Northern blot hybridization revealed that a 5.2 kb transcript hybridizes to psaA, psaB, and rps14, but not to the fMet-tRNA (trnfM) gene which follows. Primer extension and in vitro capping assays indicated that the transcriptional initiation site is 194 by upstream of psaA. The 3′ end of the transcript was determined by S1 mapping to be 105 bp downstream of rps14. The transcript is calculated to be 5,207 nucleotides long.

AB - Transcription of the psaA operon in tobacco chloroplasts has been studied. This operon contains in linear sequence the genes encoding the P700 chlorophyll a A1 and A2 apoproteins (psaA and psaB) and the gene encoding the ribosomal CS14 protein (rps14). Northern blot hybridization revealed that a 5.2 kb transcript hybridizes to psaA, psaB, and rps14, but not to the fMet-tRNA (trnfM) gene which follows. Primer extension and in vitro capping assays indicated that the transcriptional initiation site is 194 by upstream of psaA. The 3′ end of the transcript was determined by S1 mapping to be 105 bp downstream of rps14. The transcript is calculated to be 5,207 nucleotides long.

KW - Capping

KW - Chloroplast

KW - P chlorophyll a apoprotein gene

KW - Ribosomal protein gene

KW - Tobacco

KW - Transcription

UR - http://www.scopus.com/inward/record.url?scp=0024100447&partnerID=8YFLogxK

U2 - 10.1007/BF00419998

DO - 10.1007/BF00419998

M3 - 学術論文

C2 - 2850871

AN - SCOPUS:0024100447

SN - 0172-8083

VL - 14

SP - 395

EP - 400

JO - Current Genetics

JF - Current Genetics

IS - 4

ER -

Cotranscription of the genes encoding two P700 chlorophyll a apoproteins with the gene for ribosomal protein CS14: determination of the transcriptional initiation site by in vitro capping

Abstract

Keywords

ASJC Scopus subject areas

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