Cloning and characterization of a novel synaptosome-enriched mRNA that encodes 31 kDa protein

Tetsuya Ishimoto; Kensuke Ninomiya; Kazue Miyaji; Atsuko Uyeda; Michiki Kasai; Takahisa Taguchi

doi:10.1016/S0167-4781(02)00538-9

Cloning and characterization of a novel synaptosome-enriched mRNA that encodes 31 kDa protein

Tetsuya Ishimoto, Kensuke Ninomiya, Kazue Miyaji, Atsuko Uyeda, Michiki Kasai, Takahisa Taguchi^*

^*Corresponding author for this work

Molecular Neuroscience

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Although a subpopulation of mRNAs has been identified as translocated to the dendrites or the synaptic regions of neurons, the translocational mechanism has not been elucidated. To find mRNAs enriched in synapses, we compared the synaptosomal mRNAs with those from whole forebrain using differential display (DD). We cloned one of these mRNAs, which encoded a novel 31 kDa protein (PMES-2). PMES-2 mRNA was specifically transcribed in the brain and was present in the dendrites of the hippocampal neurons. PMES-2 protein was partly localized in the postsynaptic density. Although this protein is very similar to human NABC1 protein, its function is still unknown.

Original language	English
Pages (from-to)	189-195
Number of pages	7
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1579
Issue number	2-3
DOIs	https://doi.org/10.1016/S0167-4781(02)00538-9
State	Published - 2002/12/12

Keywords

Differential display
NABC1
PMES-2
Synaptosome
mRNA translocation

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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10.1016/S0167-4781(02)00538-9

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title = "Cloning and characterization of a novel synaptosome-enriched mRNA that encodes 31 kDa protein",

abstract = "Although a subpopulation of mRNAs has been identified as translocated to the dendrites or the synaptic regions of neurons, the translocational mechanism has not been elucidated. To find mRNAs enriched in synapses, we compared the synaptosomal mRNAs with those from whole forebrain using differential display (DD). We cloned one of these mRNAs, which encoded a novel 31 kDa protein (PMES-2). PMES-2 mRNA was specifically transcribed in the brain and was present in the dendrites of the hippocampal neurons. PMES-2 protein was partly localized in the postsynaptic density. Although this protein is very similar to human NABC1 protein, its function is still unknown.",

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T1 - Cloning and characterization of a novel synaptosome-enriched mRNA that encodes 31 kDa protein

AU - Ishimoto, Tetsuya

AU - Ninomiya, Kensuke

AU - Miyaji, Kazue

AU - Uyeda, Atsuko

AU - Kasai, Michiki

AU - Taguchi, Takahisa

PY - 2002/12/12

Y1 - 2002/12/12

N2 - Although a subpopulation of mRNAs has been identified as translocated to the dendrites or the synaptic regions of neurons, the translocational mechanism has not been elucidated. To find mRNAs enriched in synapses, we compared the synaptosomal mRNAs with those from whole forebrain using differential display (DD). We cloned one of these mRNAs, which encoded a novel 31 kDa protein (PMES-2). PMES-2 mRNA was specifically transcribed in the brain and was present in the dendrites of the hippocampal neurons. PMES-2 protein was partly localized in the postsynaptic density. Although this protein is very similar to human NABC1 protein, its function is still unknown.

AB - Although a subpopulation of mRNAs has been identified as translocated to the dendrites or the synaptic regions of neurons, the translocational mechanism has not been elucidated. To find mRNAs enriched in synapses, we compared the synaptosomal mRNAs with those from whole forebrain using differential display (DD). We cloned one of these mRNAs, which encoded a novel 31 kDa protein (PMES-2). PMES-2 mRNA was specifically transcribed in the brain and was present in the dendrites of the hippocampal neurons. PMES-2 protein was partly localized in the postsynaptic density. Although this protein is very similar to human NABC1 protein, its function is still unknown.

KW - Differential display

KW - NABC1

KW - PMES-2

KW - Synaptosome

KW - mRNA translocation

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U2 - 10.1016/S0167-4781(02)00538-9

DO - 10.1016/S0167-4781(02)00538-9

M3 - 学術論文

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AN - SCOPUS:0037069757

SN - 0167-4781

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JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

IS - 2-3

ER -

Cloning and characterization of a novel synaptosome-enriched mRNA that encodes 31 kDa protein

Abstract

Keywords

ASJC Scopus subject areas

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