Circadian expression and specific localization of synaptotagmin17 in the suprachiasmatic nucleus, the master circadian oscillator in mammals

Atsuko Fujioka; Mamoru Nagano; Keisuke Ikegami; Koh hei Masumoto; Tomoko Yoshikawa; Satoshi Koinuma; Ken ichi Nakahama; Yasufumi Shigeyoshi

doi:10.1016/j.brainres.2022.148129

Circadian expression and specific localization of synaptotagmin17 in the suprachiasmatic nucleus, the master circadian oscillator in mammals

Atsuko Fujioka, Mamoru Nagano, Keisuke Ikegami, Koh hei Masumoto, Tomoko Yoshikawa, Satoshi Koinuma, Ken ichi Nakahama, Yasufumi Shigeyoshi^*

^*Corresponding author for this work

Exchange Division, Organization for International Education and Exchange

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The localization and function of synaptotagmin (syt)17 in the suprachiasmatic nucleus (SCN) of the brain, which is the master circadian oscillator, were investigated. The Syt17 mRNA-containing neurons were mainly situated in the shell region while SYT17 immunoreactive cell bodies and neural fibers were detected in the core and shell of the SCN and the subparaventricular zone (SPZ). Further, electron microscopy analysis revealed SYT17 in the rough endoplasmic reticulum (rER), Golgi apparatus (G), and large and small vesicles of neurons. Syt17 mRNA expression in the SCN showed a circadian rhythm, and light exposure at night suppressed its expression. In addition, the free running period of locomotor activity rhythm was shortened in Syt17-deletion mutant mice. These findings suggest that SYT17 is involved in the regulation of circadian rhythms.

Original language	English
Article number	148129
Journal	Brain Research
Volume	1798
DOIs	https://doi.org/10.1016/j.brainres.2022.148129
State	Published - 2023/01/01

Keywords

Circadian rhythm
Immunocytochemical localization
Suprachiasmatic nucleus
Synaptotagmin17

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

Access to Document

10.1016/j.brainres.2022.148129

Cite this

@article{ea140cc6b55f40adba1d5265cabebd56,

title = "Circadian expression and specific localization of synaptotagmin17 in the suprachiasmatic nucleus, the master circadian oscillator in mammals",

abstract = "The localization and function of synaptotagmin (syt)17 in the suprachiasmatic nucleus (SCN) of the brain, which is the master circadian oscillator, were investigated. The Syt17 mRNA-containing neurons were mainly situated in the shell region while SYT17 immunoreactive cell bodies and neural fibers were detected in the core and shell of the SCN and the subparaventricular zone (SPZ). Further, electron microscopy analysis revealed SYT17 in the rough endoplasmic reticulum (rER), Golgi apparatus (G), and large and small vesicles of neurons. Syt17 mRNA expression in the SCN showed a circadian rhythm, and light exposure at night suppressed its expression. In addition, the free running period of locomotor activity rhythm was shortened in Syt17-deletion mutant mice. These findings suggest that SYT17 is involved in the regulation of circadian rhythms.",

keywords = "Circadian rhythm, Immunocytochemical localization, Suprachiasmatic nucleus, Synaptotagmin17",

author = "Atsuko Fujioka and Mamoru Nagano and Keisuke Ikegami and Masumoto, {Koh hei} and Tomoko Yoshikawa and Satoshi Koinuma and Nakahama, {Ken ichi} and Yasufumi Shigeyoshi",

note = "Publisher Copyright: {\textcopyright} 2022 Elsevier B.V.",

year = "2023",

month = jan,

day = "1",

doi = "10.1016/j.brainres.2022.148129",

language = "英語",

volume = "1798",

journal = "Brain Research",

issn = "0006-8993",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - Circadian expression and specific localization of synaptotagmin17 in the suprachiasmatic nucleus, the master circadian oscillator in mammals

AU - Fujioka, Atsuko

AU - Nagano, Mamoru

AU - Ikegami, Keisuke

AU - Masumoto, Koh hei

AU - Yoshikawa, Tomoko

AU - Koinuma, Satoshi

AU - Nakahama, Ken ichi

AU - Shigeyoshi, Yasufumi

PY - 2023/1/1

Y1 - 2023/1/1

N2 - The localization and function of synaptotagmin (syt)17 in the suprachiasmatic nucleus (SCN) of the brain, which is the master circadian oscillator, were investigated. The Syt17 mRNA-containing neurons were mainly situated in the shell region while SYT17 immunoreactive cell bodies and neural fibers were detected in the core and shell of the SCN and the subparaventricular zone (SPZ). Further, electron microscopy analysis revealed SYT17 in the rough endoplasmic reticulum (rER), Golgi apparatus (G), and large and small vesicles of neurons. Syt17 mRNA expression in the SCN showed a circadian rhythm, and light exposure at night suppressed its expression. In addition, the free running period of locomotor activity rhythm was shortened in Syt17-deletion mutant mice. These findings suggest that SYT17 is involved in the regulation of circadian rhythms.

AB - The localization and function of synaptotagmin (syt)17 in the suprachiasmatic nucleus (SCN) of the brain, which is the master circadian oscillator, were investigated. The Syt17 mRNA-containing neurons were mainly situated in the shell region while SYT17 immunoreactive cell bodies and neural fibers were detected in the core and shell of the SCN and the subparaventricular zone (SPZ). Further, electron microscopy analysis revealed SYT17 in the rough endoplasmic reticulum (rER), Golgi apparatus (G), and large and small vesicles of neurons. Syt17 mRNA expression in the SCN showed a circadian rhythm, and light exposure at night suppressed its expression. In addition, the free running period of locomotor activity rhythm was shortened in Syt17-deletion mutant mice. These findings suggest that SYT17 is involved in the regulation of circadian rhythms.

KW - Circadian rhythm

KW - Immunocytochemical localization

KW - Suprachiasmatic nucleus

KW - Synaptotagmin17

UR - http://www.scopus.com/inward/record.url?scp=85141534954&partnerID=8YFLogxK

U2 - 10.1016/j.brainres.2022.148129

DO - 10.1016/j.brainres.2022.148129

M3 - 学術論文

C2 - 36332665

AN - SCOPUS:85141534954

SN - 0006-8993

VL - 1798

JO - Brain Research

JF - Brain Research

M1 - 148129

ER -

Circadian expression and specific localization of synaptotagmin17 in the suprachiasmatic nucleus, the master circadian oscillator in mammals

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Fingerprint

Cite this