TY - JOUR
T1 - Chitosan-graft-poly(lactide) nanocarriers
T2 - An efficient antioxidant delivery system for combating oxidative stress
AU - Goshtasbi, Hamieh
AU - Awale, Suresh
AU - Amini-Fazl, Mohammad Sadegh
AU - Fathi, Marziyeh
AU - Movafeghi, Ali
AU - Barar, Jaleh
AU - Omidi, Yadollah
N1 - Publisher Copyright:
© 2024 Elsevier B.V.
PY - 2024/11
Y1 - 2024/11
N2 - Oxidative stress is a key factor in various diseases, and thus exogenous antioxidants offer effective therapeutic potential. While astaxanthin (ATX) is a potent natural antioxidant, its poor water solubility, bioavailability, and stability hinder its application. This study aimed to develop an amphiphilic chitosan-graft-poly(lactide) (CS-g-PLA) copolymer utilizing a new strategy by ring-opening polymerization of D, L-lactide via organosoluble CS/sodium dodecyl sulfate complex. Subsequently, CS-g-PLA micelles were prepared for efficient encapsulation and delivery of ATX. CS-g-PLA copolymers were characterized by FT-IR and 1H NMR. Transmission electron microscopy and dynamic light scattering revealed micellar morphology and size distribution. The antioxidant activity of CS-g-PLA/ATX was assessed using the DPPH assay, demonstrating significant improvement compared to free ATX. Furthermore, the cytotoxicity of micellar ATX was evaluated on H2O2-treated bone marrow mesenchymal stem cells (BMSCs) using MTT assay. Annexin V staining and mitochondrial membrane potential (∆Ψm) analysis revealed reduced apoptosis and enhanced protection by ATX-loaded micelles compared to free ATX. These findings suggest CS-g-PLA micelles as promising nanocarriers for ATX delivery, putatively enhancing its antioxidant potential and protecting stem cells in oxidative stress environments. This approach could hold significant implications for stem cell therapy in diseases associated with oxidative stress.
AB - Oxidative stress is a key factor in various diseases, and thus exogenous antioxidants offer effective therapeutic potential. While astaxanthin (ATX) is a potent natural antioxidant, its poor water solubility, bioavailability, and stability hinder its application. This study aimed to develop an amphiphilic chitosan-graft-poly(lactide) (CS-g-PLA) copolymer utilizing a new strategy by ring-opening polymerization of D, L-lactide via organosoluble CS/sodium dodecyl sulfate complex. Subsequently, CS-g-PLA micelles were prepared for efficient encapsulation and delivery of ATX. CS-g-PLA copolymers were characterized by FT-IR and 1H NMR. Transmission electron microscopy and dynamic light scattering revealed micellar morphology and size distribution. The antioxidant activity of CS-g-PLA/ATX was assessed using the DPPH assay, demonstrating significant improvement compared to free ATX. Furthermore, the cytotoxicity of micellar ATX was evaluated on H2O2-treated bone marrow mesenchymal stem cells (BMSCs) using MTT assay. Annexin V staining and mitochondrial membrane potential (∆Ψm) analysis revealed reduced apoptosis and enhanced protection by ATX-loaded micelles compared to free ATX. These findings suggest CS-g-PLA micelles as promising nanocarriers for ATX delivery, putatively enhancing its antioxidant potential and protecting stem cells in oxidative stress environments. This approach could hold significant implications for stem cell therapy in diseases associated with oxidative stress.
KW - Antioxidant therapy
KW - Apoptosis
KW - Astaxanthin
KW - Chitosan
KW - Micelles
KW - Oxidative stress
UR - http://www.scopus.com/inward/record.url?scp=85205572927&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2024.135280
DO - 10.1016/j.ijbiomac.2024.135280
M3 - 学術論文
C2 - 39349320
AN - SCOPUS:85205572927
SN - 0141-8130
VL - 279
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
M1 - 135280
ER -