TY - JOUR
T1 - Characterization of two oil-degrading bacterial groups in the Nakhodka oil spill
AU - Nakamura, Shogo
AU - Sakamoto, Yoshihiko
AU - Ishiyama, Miho
AU - Tanaka, Daisuke
AU - Kunii, Katsuyuki
AU - Kubo, Kazushige
AU - Sato, Chubun
N1 - Funding Information:
We thank Dr. T. Takahashi (Toho University), Dr. T. Kusui (Toyama Prefectural University), and Dr. K. Tazaki (Kanazawa University) for supplying spilled oil or direct drawn oil from the Nakhodka. This study was supported in part by Grant-in-Aids from the JSPS to S.N. (No. 12680564).
PY - 2007
Y1 - 2007
N2 - In January 1997, a heavy oil spill from the Russian tanker Nakhodka occurred in the Japan Sea. We have isolated two bacterial groups, ODB-G1 and ODB-G2, from the spilled oil. Using physiological analysis, electron microscopy, and 16S rDNA sequence analysis, ODB-G1 were shown to contain two genera, Caulobacter sp. and Alcanivorax sp. ODB-G2 also contained two genera, Halomonas sp. and Alcanivorax sp. Their abilities to degrade oil components were examined by TLC/FID method or by the change in the dry weight of the heavy oil with which they were incubated. The results obtained by the TLC/FID method showed that ODB-G1 and ODB-G2 degraded the saturate fraction and the aromatic fraction very well. They were also able to degrade the resin and the asphalten fraction, although the quantity was less. The Alcanivorax sp. degraded heavy oil well, but the Caulobacter sp. and the Halomonas sp. were found unable to degrade heavy oil as assessed by the change in the dry weight of heavy oil. Interestingly, ODB-G1 (a mixture of Caulobacter sp. and Alcanivorax sp.) and ODB-G2 (a mixture of Halomonas sp. and Alcanivorax sp.) displayed a higher degradation rate than Alcanivorax sp. alone.
AB - In January 1997, a heavy oil spill from the Russian tanker Nakhodka occurred in the Japan Sea. We have isolated two bacterial groups, ODB-G1 and ODB-G2, from the spilled oil. Using physiological analysis, electron microscopy, and 16S rDNA sequence analysis, ODB-G1 were shown to contain two genera, Caulobacter sp. and Alcanivorax sp. ODB-G2 also contained two genera, Halomonas sp. and Alcanivorax sp. Their abilities to degrade oil components were examined by TLC/FID method or by the change in the dry weight of the heavy oil with which they were incubated. The results obtained by the TLC/FID method showed that ODB-G1 and ODB-G2 degraded the saturate fraction and the aromatic fraction very well. They were also able to degrade the resin and the asphalten fraction, although the quantity was less. The Alcanivorax sp. degraded heavy oil well, but the Caulobacter sp. and the Halomonas sp. were found unable to degrade heavy oil as assessed by the change in the dry weight of heavy oil. Interestingly, ODB-G1 (a mixture of Caulobacter sp. and Alcanivorax sp.) and ODB-G2 (a mixture of Halomonas sp. and Alcanivorax sp.) displayed a higher degradation rate than Alcanivorax sp. alone.
KW - Alcanivorax sp.
KW - Biodegradation
KW - Heavy oil
KW - Nakhodka oil spill
UR - http://www.scopus.com/inward/record.url?scp=34548857916&partnerID=8YFLogxK
U2 - 10.1016/j.ibiod.2007.06.004
DO - 10.1016/j.ibiod.2007.06.004
M3 - 学術論文
AN - SCOPUS:34548857916
SN - 0964-8305
VL - 60
SP - 202
EP - 207
JO - International Biodeterioration and Biodegradation
JF - International Biodeterioration and Biodegradation
IS - 3
ER -