TY - JOUR
T1 - Characterization and biological effects of two polysaccharides isolated from Acanthopanax sciadophylloides
AU - Lee, Jung Bum
AU - Tanikawa, Tatsuya
AU - Hayashi, Kyoko
AU - Asagi, Mariko
AU - Kasahara, Yoshimasa
AU - Hayashi, Toshimitsu
N1 - Publisher Copyright:
© 2014 Elsevier Ltd.
PY - 2015/2/13
Y1 - 2015/2/13
N2 - Two polysaccharides abbreviated ANP and AAP were isolated from the young buds of Acanthopanax sciadophylloides. ANP consisted of l-arabinose, d-mannose, d-glucose and d-galactose in a ratio of ca 1.0:2.6:2.5:1.4 and its weight average molecular weight (Mw) was 1.07 × 104. AAP consisted of l-arabinose, d-galactose and 4-O-methyl-d-glucuronic acid in a ratio of ca 5:10:1, and its Mw was estimated to be 8.40 × 104. ANP was suggested to be an acetylated heteropolysaccharide, whereas AAP was speculated to be a type II arabinogalactan on the basis of structural analysis data. Both polysaccharides were found to stimulate NO production and induce the expression of cytokine mRNAs including IL-1β, IL-6, IL-10 and TNF-α on RAW264.7 cells. They also induced NF-κB activation in RAW-Blue cells. NO production and NF-κB activation by both polysaccharides were decreased by pretreatment with neutralizing anti-TLR-4 and anti-CD14 antibodies but not with anti-TLR-2, anti-SR-A, anti-CD11c, and anti-Dectin-1 antibodies. Therefore, these immunostimulating effects of ANP and AAP were suggested to be promoted by the interaction through the membrane receptors, TLR-4 and CD14. In addition to immunomodulating effects, ANP showed anti-HSV-2 effects in vitro.
AB - Two polysaccharides abbreviated ANP and AAP were isolated from the young buds of Acanthopanax sciadophylloides. ANP consisted of l-arabinose, d-mannose, d-glucose and d-galactose in a ratio of ca 1.0:2.6:2.5:1.4 and its weight average molecular weight (Mw) was 1.07 × 104. AAP consisted of l-arabinose, d-galactose and 4-O-methyl-d-glucuronic acid in a ratio of ca 5:10:1, and its Mw was estimated to be 8.40 × 104. ANP was suggested to be an acetylated heteropolysaccharide, whereas AAP was speculated to be a type II arabinogalactan on the basis of structural analysis data. Both polysaccharides were found to stimulate NO production and induce the expression of cytokine mRNAs including IL-1β, IL-6, IL-10 and TNF-α on RAW264.7 cells. They also induced NF-κB activation in RAW-Blue cells. NO production and NF-κB activation by both polysaccharides were decreased by pretreatment with neutralizing anti-TLR-4 and anti-CD14 antibodies but not with anti-TLR-2, anti-SR-A, anti-CD11c, and anti-Dectin-1 antibodies. Therefore, these immunostimulating effects of ANP and AAP were suggested to be promoted by the interaction through the membrane receptors, TLR-4 and CD14. In addition to immunomodulating effects, ANP showed anti-HSV-2 effects in vitro.
KW - Acanthopanax sciadophylloides
KW - Anti-herpetic activity
KW - Immunostimulation
KW - Polysaccharide
UR - http://www.scopus.com/inward/record.url?scp=84910646194&partnerID=8YFLogxK
U2 - 10.1016/j.carbpol.2014.04.013
DO - 10.1016/j.carbpol.2014.04.013
M3 - 学術論文
C2 - 25458285
AN - SCOPUS:84910646194
SN - 0144-8617
VL - 116
SP - 159
EP - 166
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
ER -