TY - JOUR
T1 - Change in expression of a 90-kDa glycoprotein GP90-MC301 during prenatal and postnatal testicular development
T2 - A differentiation marker for rat germ cells
AU - Tanii, Ichiro
AU - Yoshinaga, Kazuya
N1 - Funding Information:
Acknowledgements We would like to thank Dr. K. Toshimori for his support during the course of this project. This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan.
PY - 2002
Y1 - 2002
N2 - GP90-MC301, a 90-kDa glycoprotein recognized by the monoclonal antibody MC301, is a reliable stage-specific marker for preleptotene to pachytene spermatocytes in adult rat testes. In this study we confirmed that the glycoprotein is also useful as a marker for germ cells in prenatal and postnatal testes. Immunohistochemical analysis showed a dramatic change in GP90-MC301 expression in germ cells during testis development. Strong expression was detected in primordial germ cells at embryonic day (E) 13 and in gonocytes at E16, and the expression was then markedly reduced at around the time (E18) gonocytes undergo G1/G0 arrest, and was not restored in gonocytes or spermatogonia afterward. Thereafter, it reappeared in primary spermatocytes in the prepubertal period. Testicular somatic cells such as Sertoli cells, Leydig cells, and peritubular myoid cells expressed GP90-MC301 during specific periods which were largely correlated with periods of active proliferation of these testicular somatic cells. Western blotting showed that GP90-MC301 was expressed during testis development without a change in its molecular size. Thus, GP90-MC301 is potentially useful for the analysis of not only spermatogenesis but also early testis development.
AB - GP90-MC301, a 90-kDa glycoprotein recognized by the monoclonal antibody MC301, is a reliable stage-specific marker for preleptotene to pachytene spermatocytes in adult rat testes. In this study we confirmed that the glycoprotein is also useful as a marker for germ cells in prenatal and postnatal testes. Immunohistochemical analysis showed a dramatic change in GP90-MC301 expression in germ cells during testis development. Strong expression was detected in primordial germ cells at embryonic day (E) 13 and in gonocytes at E16, and the expression was then markedly reduced at around the time (E18) gonocytes undergo G1/G0 arrest, and was not restored in gonocytes or spermatogonia afterward. Thereafter, it reappeared in primary spermatocytes in the prepubertal period. Testicular somatic cells such as Sertoli cells, Leydig cells, and peritubular myoid cells expressed GP90-MC301 during specific periods which were largely correlated with periods of active proliferation of these testicular somatic cells. Western blotting showed that GP90-MC301 was expressed during testis development without a change in its molecular size. Thus, GP90-MC301 is potentially useful for the analysis of not only spermatogenesis but also early testis development.
KW - G1/G0 arrest
KW - Gonocytes
KW - Meiosis
KW - Oocytes
KW - Spermatocytes
UR - http://www.scopus.com/inward/record.url?scp=0036434245&partnerID=8YFLogxK
U2 - 10.1007/s00418-002-0468-1
DO - 10.1007/s00418-002-0468-1
M3 - 学術論文
C2 - 12432452
AN - SCOPUS:0036434245
SN - 0948-6143
VL - 118
SP - 409
EP - 414
JO - Histochemistry and Cell Biology
JF - Histochemistry and Cell Biology
IS - 5
ER -