TY - JOUR
T1 - CD271 mRNA/hnRNPA2B1 complex promotes proliferation and stemness in oral and head and neck squamous cell carcinoma
AU - Mochizuki, Mai
AU - Shibuya-Takahashi, Rie
AU - Kanno, Shin Ichiro
AU - Adachi, Shungo
AU - Fujimori, Haruna
AU - Nakazato, Akira
AU - Fujii, Keitaro
AU - Morita, Shinkichi
AU - Saijoh, Satoshi
AU - Yamazaki, Tomoko
AU - Imai, Takayuki
AU - Asada, Yukinori
AU - Yamaguchi, Kazunori
AU - Yasuda, Jun
AU - Shindo, Norihisa
AU - Sugamura, Kazuo
AU - Tamai, Keiichi
N1 - Publisher Copyright:
© 2024 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.
PY - 2024/7
Y1 - 2024/7
N2 - RNAs, such as noncoding RNA, microRNA, and recently mRNA, have been recognized as signal transduction molecules. CD271, also known as nerve growth factor receptor, has a critical role in cancer, although the precise mechanism is still unclear. Here, we show that CD271 mRNA, but not CD271 protein, facilitates spheroid cell proliferation. We established CD271−/− cells lacking both mRNA and protein of CD271, as well as CD271 protein knockout cells lacking only CD271 protein, from hypopharyngeal and oral squamous cell carcinoma lines. Sphere formation was reduced in CD271−/− cells but not in CD271 protein knockout cells. Mutated CD271 mRNA, which is not translated to a protein, promoted sphere formation. CD271 mRNA bound to hnRNPA2B1 protein at the 3′-UTR region, and the inhibition of this interaction reduced sphere formation. In surgical specimens, the CD271 mRNA/protein expression ratio was higher in the cancerous area than in the noncancerous area. These data suggest CD271 mRNA has dual functions, encompassing protein-coding and noncoding roles, with its noncoding RNA function being predominant in oral and head and neck squamous cell carcinoma.
AB - RNAs, such as noncoding RNA, microRNA, and recently mRNA, have been recognized as signal transduction molecules. CD271, also known as nerve growth factor receptor, has a critical role in cancer, although the precise mechanism is still unclear. Here, we show that CD271 mRNA, but not CD271 protein, facilitates spheroid cell proliferation. We established CD271−/− cells lacking both mRNA and protein of CD271, as well as CD271 protein knockout cells lacking only CD271 protein, from hypopharyngeal and oral squamous cell carcinoma lines. Sphere formation was reduced in CD271−/− cells but not in CD271 protein knockout cells. Mutated CD271 mRNA, which is not translated to a protein, promoted sphere formation. CD271 mRNA bound to hnRNPA2B1 protein at the 3′-UTR region, and the inhibition of this interaction reduced sphere formation. In surgical specimens, the CD271 mRNA/protein expression ratio was higher in the cancerous area than in the noncancerous area. These data suggest CD271 mRNA has dual functions, encompassing protein-coding and noncoding roles, with its noncoding RNA function being predominant in oral and head and neck squamous cell carcinoma.
KW - CD271
KW - head and neck squamous cell carcinoma
KW - hnRNPA2B1
KW - mRNA
KW - oral squamous cell carcinoma
UR - http://www.scopus.com/inward/record.url?scp=85192232837&partnerID=8YFLogxK
U2 - 10.1111/cas.16187
DO - 10.1111/cas.16187
M3 - 学術論文
C2 - 38710200
AN - SCOPUS:85192232837
SN - 1347-9032
VL - 115
SP - 2346
EP - 2359
JO - Cancer Science
JF - Cancer Science
IS - 7
ER -
