Catalytic RNA nano-objects formed by self-assembly of group I ribozyme dimers serving as unit structures

Ryuji Kiyooka; Junya Akagi; Kumi Hidaka; Hiroshi Sugiyama; Masayuki Endo; Shigeyoshi Matsumura; Yoshiya Ikawa

doi:10.1016/j.jbiosc.2020.04.010

Catalytic RNA nano-objects formed by self-assembly of group I ribozyme dimers serving as unit structures

Ryuji Kiyooka, Junya Akagi, Kumi Hidaka, Hiroshi Sugiyama, Masayuki Endo, Shigeyoshi Matsumura, Yoshiya Ikawa^*

^*Corresponding author for this work

Program of Chemistry

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Ribozymes with modular structures are attractive platforms for the construction of nanoscale RNA objects with biological functions. We designed group I ribozyme dimers as unit ribozyme dimers (Urds), which self-assembled to form their polymeric states and also oligomeric states with defined numbers of Urds. Assembly of Urds yielded catalytic ability of a pair of distinct ribozyme units to cleave two distinct substrates. The morphologies of the assembled ribozyme structures were observed directly by atomic force microscopy (AFM).

Original language	English
Pages (from-to)	253-259
Number of pages	7
Journal	Journal of Bioscience and Bioengineering
Volume	130
Issue number	3
DOIs	https://doi.org/10.1016/j.jbiosc.2020.04.010
State	Published - 2020/09

Keywords

Catalytic RNA
Group I intron
RNA nanostructure
RNA nanotechnology
Ribozyme

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1016/j.jbiosc.2020.04.010

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title = "Catalytic RNA nano-objects formed by self-assembly of group I ribozyme dimers serving as unit structures",

abstract = "Ribozymes with modular structures are attractive platforms for the construction of nanoscale RNA objects with biological functions. We designed group I ribozyme dimers as unit ribozyme dimers (Urds), which self-assembled to form their polymeric states and also oligomeric states with defined numbers of Urds. Assembly of Urds yielded catalytic ability of a pair of distinct ribozyme units to cleave two distinct substrates. The morphologies of the assembled ribozyme structures were observed directly by atomic force microscopy (AFM).",

keywords = "Catalytic RNA, Group I intron, RNA nanostructure, RNA nanotechnology, Ribozyme",

author = "Ryuji Kiyooka and Junya Akagi and Kumi Hidaka and Hiroshi Sugiyama and Masayuki Endo and Shigeyoshi Matsumura and Yoshiya Ikawa",

note = "Publisher Copyright: {\textcopyright} 2020 The Society for Biotechnology, Japan",

year = "2020",

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doi = "10.1016/j.jbiosc.2020.04.010",

language = "英語",

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T1 - Catalytic RNA nano-objects formed by self-assembly of group I ribozyme dimers serving as unit structures

AU - Kiyooka, Ryuji

AU - Akagi, Junya

AU - Hidaka, Kumi

AU - Sugiyama, Hiroshi

AU - Endo, Masayuki

AU - Matsumura, Shigeyoshi

AU - Ikawa, Yoshiya

PY - 2020/9

Y1 - 2020/9

N2 - Ribozymes with modular structures are attractive platforms for the construction of nanoscale RNA objects with biological functions. We designed group I ribozyme dimers as unit ribozyme dimers (Urds), which self-assembled to form their polymeric states and also oligomeric states with defined numbers of Urds. Assembly of Urds yielded catalytic ability of a pair of distinct ribozyme units to cleave two distinct substrates. The morphologies of the assembled ribozyme structures were observed directly by atomic force microscopy (AFM).

AB - Ribozymes with modular structures are attractive platforms for the construction of nanoscale RNA objects with biological functions. We designed group I ribozyme dimers as unit ribozyme dimers (Urds), which self-assembled to form their polymeric states and also oligomeric states with defined numbers of Urds. Assembly of Urds yielded catalytic ability of a pair of distinct ribozyme units to cleave two distinct substrates. The morphologies of the assembled ribozyme structures were observed directly by atomic force microscopy (AFM).

KW - Catalytic RNA

KW - Group I intron

KW - RNA nanostructure

KW - RNA nanotechnology

KW - Ribozyme

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AN - SCOPUS:85085207521

SN - 1389-1723

VL - 130

SP - 253

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JO - Journal of Bioscience and Bioengineering

JF - Journal of Bioscience and Bioengineering

IS - 3

ER -

Catalytic RNA nano-objects formed by self-assembly of group I ribozyme dimers serving as unit structures

Abstract

Keywords

ASJC Scopus subject areas

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