Ca2+-activated outward-rectifier K+ channels and histamine release by rat gastric enterochromaffin-like cells

Hideki Sakai; Yoshiaki Tabuchi; Bunpei Kakinoki; Hisayuki Seike; Shigeyo Kumagai; Chika Matsumoto; Noriaki Takeguchi

doi:10.1016/0922-4106(95)90137-X

Ca²⁺-activated outward-rectifier K⁺ channels and histamine release by rat gastric enterochromaffin-like cells

Hideki Sakai^*, Yoshiaki Tabuchi, Bunpei Kakinoki, Hisayuki Seike, Shigeyo Kumagai, Chika Matsumoto, Noriaki Takeguchi

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Gastric enterochromaffin-like (ECL) cells were isolated from rat gastric fundic mucosa by Percoll density-gradient centrifugation and counter-flow elutriation. About 67% of cells in the purified cell suspension were ECL cells, which were reacted with anti-histidine decarboxylase antibody. A23187, a calcium ionophore, at 0.1-10 μM induced histamine release from the ECL cell-rich suspension, indicating that the Ca²⁺ pathway is involved in the mechanism of histamine release from ECL cells. A23187 at 5 μM significantly increased outward-rectifier cationic current in 62% of cells in the ECL cell-rich fraction. A23187-sensitive cells showed acridine orange uptake. In single-channel recordings, a Ca²⁺-dependent outward-rectifier K⁺ channel of large conductance (146 ± 22 picosiemens) was found in the cell that showed acridine orange uptake. The channel opened in a voltage-dependent manner at 0.1 μM of intracellular free Ca²⁺ concentration. These results may suggest that opening of the Ca²⁺-activated K⁺ channel is one of the steps involved in the mechanism of histamine release in ECL cells.

Original language	English
Pages (from-to)	153-158
Number of pages	6
Journal	European Journal of Pharmacology: Molecular Pharmacology
Volume	291
Issue number	2
DOIs	https://doi.org/10.1016/0922-4106(95)90137-X
State	Published - 1995/10/15

Keywords

Ca ionophore
Enterochromaffin-like cell
Histamine
K channel
Patch-clamp
Stomach, rat

ASJC Scopus subject areas

Pharmacology

Access to Document

10.1016/0922-4106(95)90137-X

Cite this

@article{4c4c71977a324bc7892d56e617339977,

title = "Ca2+-activated outward-rectifier K+ channels and histamine release by rat gastric enterochromaffin-like cells",

abstract = "Gastric enterochromaffin-like (ECL) cells were isolated from rat gastric fundic mucosa by Percoll density-gradient centrifugation and counter-flow elutriation. About 67% of cells in the purified cell suspension were ECL cells, which were reacted with anti-histidine decarboxylase antibody. A23187, a calcium ionophore, at 0.1-10 μM induced histamine release from the ECL cell-rich suspension, indicating that the Ca2+ pathway is involved in the mechanism of histamine release from ECL cells. A23187 at 5 μM significantly increased outward-rectifier cationic current in 62% of cells in the ECL cell-rich fraction. A23187-sensitive cells showed acridine orange uptake. In single-channel recordings, a Ca2+-dependent outward-rectifier K+ channel of large conductance (146 ± 22 picosiemens) was found in the cell that showed acridine orange uptake. The channel opened in a voltage-dependent manner at 0.1 μM of intracellular free Ca2+ concentration. These results may suggest that opening of the Ca2+-activated K+ channel is one of the steps involved in the mechanism of histamine release in ECL cells.",

keywords = "Ca ionophore, Enterochromaffin-like cell, Histamine, K channel, Patch-clamp, Stomach, rat",

author = "Hideki Sakai and Yoshiaki Tabuchi and Bunpei Kakinoki and Hisayuki Seike and Shigeyo Kumagai and Chika Matsumoto and Noriaki Takeguchi",

note = "Funding Information: This work was supported m part by Grants-m-Aid for Encouragement of Young Sc~enUsts (to H S ), Scientific Research (B) (to N T ), and Scientific Research on Priority Areas (to N T ) from the Ministry of Education, Science and Culture of Japan, and by grants from the ONO Medt-cal Research Foundation, Salt Science Research Foundation and Clba-Gelgy Foundation",

year = "1995",

month = oct,

day = "15",

doi = "10.1016/0922-4106(95)90137-X",

language = "英語",

volume = "291",

pages = "153--158",

journal = "European Journal of Pharmacology: Molecular Pharmacology",

issn = "0922-4106",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Ca2+-activated outward-rectifier K+ channels and histamine release by rat gastric enterochromaffin-like cells

AU - Sakai, Hideki

AU - Tabuchi, Yoshiaki

AU - Kakinoki, Bunpei

AU - Seike, Hisayuki

AU - Kumagai, Shigeyo

AU - Matsumoto, Chika

AU - Takeguchi, Noriaki

N1 - Funding Information: This work was supported m part by Grants-m-Aid for Encouragement of Young Sc~enUsts (to H S ), Scientific Research (B) (to N T ), and Scientific Research on Priority Areas (to N T ) from the Ministry of Education, Science and Culture of Japan, and by grants from the ONO Medt-cal Research Foundation, Salt Science Research Foundation and Clba-Gelgy Foundation

PY - 1995/10/15

Y1 - 1995/10/15

N2 - Gastric enterochromaffin-like (ECL) cells were isolated from rat gastric fundic mucosa by Percoll density-gradient centrifugation and counter-flow elutriation. About 67% of cells in the purified cell suspension were ECL cells, which were reacted with anti-histidine decarboxylase antibody. A23187, a calcium ionophore, at 0.1-10 μM induced histamine release from the ECL cell-rich suspension, indicating that the Ca2+ pathway is involved in the mechanism of histamine release from ECL cells. A23187 at 5 μM significantly increased outward-rectifier cationic current in 62% of cells in the ECL cell-rich fraction. A23187-sensitive cells showed acridine orange uptake. In single-channel recordings, a Ca2+-dependent outward-rectifier K+ channel of large conductance (146 ± 22 picosiemens) was found in the cell that showed acridine orange uptake. The channel opened in a voltage-dependent manner at 0.1 μM of intracellular free Ca2+ concentration. These results may suggest that opening of the Ca2+-activated K+ channel is one of the steps involved in the mechanism of histamine release in ECL cells.

AB - Gastric enterochromaffin-like (ECL) cells were isolated from rat gastric fundic mucosa by Percoll density-gradient centrifugation and counter-flow elutriation. About 67% of cells in the purified cell suspension were ECL cells, which were reacted with anti-histidine decarboxylase antibody. A23187, a calcium ionophore, at 0.1-10 μM induced histamine release from the ECL cell-rich suspension, indicating that the Ca2+ pathway is involved in the mechanism of histamine release from ECL cells. A23187 at 5 μM significantly increased outward-rectifier cationic current in 62% of cells in the ECL cell-rich fraction. A23187-sensitive cells showed acridine orange uptake. In single-channel recordings, a Ca2+-dependent outward-rectifier K+ channel of large conductance (146 ± 22 picosiemens) was found in the cell that showed acridine orange uptake. The channel opened in a voltage-dependent manner at 0.1 μM of intracellular free Ca2+ concentration. These results may suggest that opening of the Ca2+-activated K+ channel is one of the steps involved in the mechanism of histamine release in ECL cells.

KW - Ca ionophore

KW - Enterochromaffin-like cell

KW - Histamine

KW - K channel

KW - Patch-clamp

KW - Stomach, rat

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JO - European Journal of Pharmacology: Molecular Pharmacology

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