TY - JOUR
T1 - Calbindin is predominantly expressed in nitrergic neurons in rat esophagus
AU - Kuramoto, Hirofumi
AU - Kadowaki, Makoto
AU - Yamamoto, Takeshi
AU - Kuwano, Ryozo
N1 - Funding Information:
The authors thank Dr. P.C. Emson (The Babraham Institute, Babraham, UK) for kindly providing the NOS antiserum. This study was supported by Grant-in-Aids for Scientific Research from the Ministry of Education, Sports, and Culture of Japan to H.K. (No. 15500236) and the Smoking Research Foundation to M.K.
PY - 2006/6/19
Y1 - 2006/6/19
N2 - We used immunohistochemistry to investigate the colocalization pattern of calbindin D28k (Calb) with nitric oxide and acetylcholine in myenteric neurons in the rat esophagus and compared it to that in the ileum or distal colon. The proportion of Calb-immunoreactive (IR) neurons to the total neurons in the esophagus (8%) was lower than that in the ileum (38%) or distal colon (27%). A majority (84%) of the esophageal Calb-IR neurons were uniaxonal neurons. On the other hand, 88% and 66% of Calb-IR neurons in the ileum and distal colon, respectively, had Dogiel type II morphology, while most of the others were Dogiel type I neurons. Double immunolabeling indicated that most (87%) of the esophageal Calb-IR neurons were nitric oxide synthase (NOS) positive and a minority (21%) were choline acetyltransferase (ChAT) positive. Most (93% and 89%, respectively) of the Calb-IR neurons in the ileum and distal colon showed ChAT immunoreactivity and only a small number exhibited NOS immunoreactivity in the ileum and distal colon. In the esophagus, some of Calb-IR nerve endings surrounding the myenteric neurons were NOS positive, but no Calb immunoreactivity was found on the motor endplates of the striated muscles. Therefore, the present study revealed that most of the Calb-IR neurons in the esophagus are nitrergic, and it suggested that the Calb-IR neurons might be primarily involved in interneuronal roles in the esophageal nervous system.
AB - We used immunohistochemistry to investigate the colocalization pattern of calbindin D28k (Calb) with nitric oxide and acetylcholine in myenteric neurons in the rat esophagus and compared it to that in the ileum or distal colon. The proportion of Calb-immunoreactive (IR) neurons to the total neurons in the esophagus (8%) was lower than that in the ileum (38%) or distal colon (27%). A majority (84%) of the esophageal Calb-IR neurons were uniaxonal neurons. On the other hand, 88% and 66% of Calb-IR neurons in the ileum and distal colon, respectively, had Dogiel type II morphology, while most of the others were Dogiel type I neurons. Double immunolabeling indicated that most (87%) of the esophageal Calb-IR neurons were nitric oxide synthase (NOS) positive and a minority (21%) were choline acetyltransferase (ChAT) positive. Most (93% and 89%, respectively) of the Calb-IR neurons in the ileum and distal colon showed ChAT immunoreactivity and only a small number exhibited NOS immunoreactivity in the ileum and distal colon. In the esophagus, some of Calb-IR nerve endings surrounding the myenteric neurons were NOS positive, but no Calb immunoreactivity was found on the motor endplates of the striated muscles. Therefore, the present study revealed that most of the Calb-IR neurons in the esophagus are nitrergic, and it suggested that the Calb-IR neurons might be primarily involved in interneuronal roles in the esophageal nervous system.
KW - Calbindin
KW - Choline acetyltransferase
KW - Esophagus
KW - Immunohistochemistry
KW - Myenteric neurons
KW - Nitric oxide synthase
UR - http://www.scopus.com/inward/record.url?scp=33646430076&partnerID=8YFLogxK
U2 - 10.1016/j.neulet.2006.03.019
DO - 10.1016/j.neulet.2006.03.019
M3 - 学術論文
C2 - 16600497
AN - SCOPUS:33646430076
SN - 0304-3940
VL - 401
SP - 174
EP - 177
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 1-2
ER -