Application of Novel Sensors for Bioprocess On-Line Monitoring to Dextran Fermentation

Hideaki Endo, Masayasu Suzuki, Koji Sode, Eiichi Tamiya, Isao Karube

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

An on-line monitoring system was developed for the measurement of various parameters such as cell concentration, viscosity of broth and sucrose concentration in dextran fermentation. This system was constructed from a piezoelectric gum sensor, a quartz crystal viscous sensor, a sucrose sensor, a bioreactor and a personal computer. A piezoelectric gum sensor for the determination of biomass was prepared with two piezoelectric gums, a pulse generator and an amplifier. The calibration curve for L. mesenteroides was linear in the range of 1.0-4.8 g dry cell mass·l-1. A quartz crystal viscous sensor was prepared with AT-cut quartz crystal, an oscillating circuit and a peak level meter. A linear relationship was obtained between resonant resistance (R1) of the quartz crystal and (ρη)1/2 of dextran solution, where ρ and η were density and viscosity respectively. A sucrose sensor was prepared with three immobilized enzyme layers (glucose oxidase, mutarotase and invertase) and a gold working electrode. The calibration curve for sucrose was linear up to 1000 mg·l-1. An on-line monitoring system was applied to dextran fermentation, and these paramenters were measured. Good correlation was obtained between the sensor signals and the values determined by conventional methods.

Original languageEnglish
Pages (from-to)559-564
Number of pages6
JournalKagaku Kogaku Ronbunshu
Volume17
Issue number3
DOIs
StatePublished - 1991

Keywords

  • Bioprocess
  • Cell Concentration
  • Dextran
  • On-Line Monitoring
  • Sensor
  • Sucrose
  • Viscosity

ASJC Scopus subject areas

  • General Chemistry
  • General Chemical Engineering

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