Abstract
An on-line monitoring system was developed for the measurement of various parameters such as cell concentration, viscosity of broth and sucrose concentration in dextran fermentation. This system was constructed from a piezoelectric gum sensor, a quartz crystal viscous sensor, a sucrose sensor, a bioreactor and a personal computer. A piezoelectric gum sensor for the determination of biomass was prepared with two piezoelectric gums, a pulse generator and an amplifier. The calibration curve for L. mesenteroides was linear in the range of 1.0-4.8 g dry cell mass·l-1. A quartz crystal viscous sensor was prepared with AT-cut quartz crystal, an oscillating circuit and a peak level meter. A linear relationship was obtained between resonant resistance (R1) of the quartz crystal and (ρη)1/2 of dextran solution, where ρ and η were density and viscosity respectively. A sucrose sensor was prepared with three immobilized enzyme layers (glucose oxidase, mutarotase and invertase) and a gold working electrode. The calibration curve for sucrose was linear up to 1000 mg·l-1. An on-line monitoring system was applied to dextran fermentation, and these paramenters were measured. Good correlation was obtained between the sensor signals and the values determined by conventional methods.
Original language | English |
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Pages (from-to) | 559-564 |
Number of pages | 6 |
Journal | Kagaku Kogaku Ronbunshu |
Volume | 17 |
Issue number | 3 |
DOIs | |
State | Published - 1991 |
Keywords
- Bioprocess
- Cell Concentration
- Dextran
- On-Line Monitoring
- Sensor
- Sucrose
- Viscosity
ASJC Scopus subject areas
- General Chemistry
- General Chemical Engineering